Glutathione-S-transferase-catalyzed reaction of glutathione for electrochemical biosensing of temephos, fenobucarb and dimethoate

Borah, Himadri; Dutta, Rekha Rani; Gogoi, Sweety; Medhi, Tapas; Puzari, Panchanan

doi:10.1039/c7ay01258f

Cited by 14 publications

(6 citation statements)

References 65 publications

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“…Efforts are now underway to address this shortcoming. 70 Borah et al fabricated an amperometric biosensor based on the inhibition of the enzyme glutathione S-transferase (GST). 71 The enzyme was immobilized on the surface of the platinum working electrode with the aid of a graphene oxide− gelatin matrix.…”

Section: Environmental Toxicant Detection Usingmentioning

confidence: 99%

“…Although the specificity of enzyme-based biosensors is advantageous, it has hindered their use in multianalyte detection. Efforts are now underway to address this shortcoming . Borah et al fabricated an amperometric biosensor based on the inhibition of the enzyme glutathione S -transferase (GST) .…”

Section: Environmental Toxicant Detection Using Electrochemical Biose...mentioning

confidence: 99%

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Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Hara¹,

Singh

2021

ACS EST Water

127

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The release of chemicals into water systems has resulted in pollution in many parts of the world, threatening human health and aquatic ecosystems. Sources of chemical discharge include industry, agricultural, wastewater treatment plants, and stormwater overflows. To combat water pollution, the European Union has introduced several directives that set concentration limits for chemicals in drinking water, surface water, and groundwater. To meet these limits, it is essential that rapid, reliable, and sensitive analytical detection systems be developed and put into use. This Review presents the progress made in the development of electrochemical biosensors for environmental toxicants (pesticides and heavy metals) over the past seven years (2014–2020). For those unfamiliar with this field of research, the concept of a biosensor is introduced followed by a critical evaluation of their performance in detecting the toxicants. Current challenges are discussed as well as potential avenues for future research, including the demands for enhanced analytical performance, improved biosensor stability and shelf life, and greater integration with microfluidic devices and wireless database technologies for remote environmental sensing applications. We believe that this Review will be beneficial and enhance awareness and appreciation of the role that electrochemical biosensors can play in protecting our environment and water resources.

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Section: Environmental Toxicant Detection Usingmentioning

confidence: 99%

Section: Environmental Toxicant Detection Using Electrochemical Biose...mentioning

confidence: 99%

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Hara¹,

Singh

2021

ACS EST Water

127

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“…Among such methods, electrochemical methods are found to be the candidate of choice and are extensively studied. 7–10…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of 4,6-dinitro-o-cresol based on a glassy carbon electrode modified with Zr-UiO-66 metal–organic framework entrapped FMWCNTs

Hazarika,

Deffo,

Wamba

et al. 2024

Anal. Methods

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“…GST was used as the recognition element to detect captan and glutathione in prior studies. Others used non-GSH capture molecules to detect GST protein and its function. , Electrochemical and aggregate-induction sensor platforms were used in these studies. , More broadly, optical biosensors for other enzymes have been demonstrated to detect renal and hepatic injury, including for caspases, glucosaminidase, and galactosidase. − We believe that a rapid, label-free optical sensor for fusion protein tags, which can access the media of industrial protein expression systems, would confer a significant benefit to biotechnology and molecular biology fields.…”

Section: Introductionmentioning

confidence: 99%

“…Others used non-GSH capture molecules to detect GST protein and its function. 18,19 Electrochemical and aggregate-induction sensor platforms were used in these studies. 17,20 More broadly, optical biosensors for other enzymes have been demonstrated to detect renal and hepatic injury, including for caspases, glucosaminidase, and galactosidase.…”

Section: ■ Introductionmentioning

confidence: 99%

Glutathione-S-transferase Fusion Protein Nanosensor

et al. 2020

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Fusion protein tags are widely used to capture and track proteins in research and industrial bioreactor processes. Quantifying fusion-tagged proteins normally requires several purification steps coupled with classical protein assays. Here, we developed a broadly applicable nanosensor platform that quantifies glutathione-S-transferase (GST) fusion proteins in real-time. We synthesized a glutathione-DNA-carbon nanotube system to investigate glutathione-GST interactions via semiconducting single-walled carbon nanotube (SWCNT) photoluminescence. We found that SWCNT fluorescence wavelength and intensity modulation occurred specifically in response to GST and GST-fusions. The sensor response was dependent on SWCNT structure, wherein mod(n – m, 3) = 1 nanotube wavelength and intensity responses correlated with nanotube diameter distinctly from mod(n – m, 3) = 2 SWCNT responses. We also found broad functionality of this sensor to diverse GST-tagged proteins. This work comprises the first label-free optical sensor for GST and has implications for the assessment of protein expression in situ, including in imaging and industrial bioreactor settings.

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Glutathione-S-transferase-catalyzed reaction of glutathione for electrochemical biosensing of temephos, fenobucarb and dimethoate

Abstract: This study describes a sensitive bio-electrochemical detection method for extensively used toxic organothiophosphate pesticides temephos and dimethoate, and organocarbamate fenobucarb, by employing a simple mediatorless cyclic voltammetric technique.

Cited by 14 publications

References 65 publications

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Sensitive determination of 4,6-dinitro-o-cresol based on a glassy carbon electrode modified with Zr-UiO-66 metal–organic framework entrapped FMWCNTs

Glutathione-S-transferase Fusion Protein Nanosensor

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