Glutathione S-Transferase as Predictor of Functional Outcome in Transplantation of Machine-Preserved Non-Heart-Beating Donor Kidneys

Jw, Daemen; Ap, Oomen; Ma, Janssen; L, van de Schoot; Bk, van Kreel; Heineman, Erik; Kootstra, G.

doi:10.1097/00007890-199701150-00017

Cited by 119 publications

(61 citation statements)

References 19 publications

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“…35 Therefore the value of urinary α-GST may reflect the number of tubular cells damaged. 36 In fresh kidney biopsies α-GST is found exclusively in the proximal tubules. 15 Tubular injury is the only phenomenon known to cause an increase in urinary α-GST.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Diltiazem may preserve renal tubular integrity after cardiac surgery

Piper

Kumle

Maleck

et al. 2003

Can J Anesth/J Can Anesth

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P Pu ur rp po os se e: : To evaluate the influence of dopamine and diltiazem on renal function and markers for acute renal failure, including urinary alpha-glutathion s-transferase (α-GST), alpha-1-microglobulin (α 1 -MG) and N-acetyl-ß-glucosaminidase (ß-NAG) after extracorporeal circulation.M Me et th ho od ds s: : In a randomized, placebo-controlled, double-blind trial we evaluated the efficacy of dopamine (2.5 µg·kg -1 ·min -1 ), diltiazem (2 µg·kg -1 ·min -1 ) or placebo administered over 48 hr postoperatively to maintain renal tubular integrity in 60 elective cardiac surgery patients. α-GST, α 1 -MG, ß-NAG, and creatinine clearance were measured from urine collected during surgery (T0), the first four hours (T1), 24 hr (T2) and 48 hr (T3) postoperatively.R Re es su ul lt ts s: : Cumulative urine output in the diltiazem group (9.0 ± 2.8 L) increased significantly compared with placebo (7.0 ± 1.6 L), but not compared with dopamine (7.8 ± 1.8 L). Creatinine clearance showed no significant intergroup differences. In all groups α 1 -MG increased from T0 to T3, but we found no significant intergroup differences. α-GST increased significantly from T0 to T3 in the placebo (2.1 ± 1.8 to 11.4 ± 8.6 µg·L -1 ) and in the dopamine groups (2.7 ± 1.8 to 13.6 ± 14.9 µg·L -1 ), but not in the diltiazem group (1.8 ± 1.4 to 3.2 ± 3.2 µg·L -1 ). Forty-eight hours postoperatively α-GST was significantly lower in the diltiazem group than in both other groups.C Co on nc cl lu us si io on ns s: : Diltiazem stimulates urine output, reduces excretion of α-GST and ß-NAG and may be useful to maintain tubular integrity after cardiac surgery. Objectif : Évaluer l'influence de la dopamine et du diltiazem sur la fonction rénale et les paramètres de l`insuffisance rénale aiguë, y compris l'alpha-glutathion S-transférase (α -GST), l'alpha-microglobuline (α 1 -MG) et la N-acétyl-ß glucosaminidase (ß-NAG) urinaires après circulation extracorporelle. Méthode : Soixante patients opérés pour un pontage aortocoronarien ont participé à un essai randomisé en double aveugle et contrôlé contre placebo pour mesurer l'efficacité de la dopamine (2,5 µg kg -1 min -1 ), du diltiazem (2 µg kg -1 min -1 ) ou d'un placebo administrés pendant 48 h postopératoires pour maintenir l'intégrité tubulaire rénale. L' α-GST, l' α 1 -MG et la ß-NAG et la clairance de la créatinine ont été mesurés dans l'urine recueillie pendant l'intervention chirurgicale (T0), quatre heures (T1), 24 h (T2) et 48 h (T3) après l'opération.Résultats : L'excrétion urinaire cumulative a augmenté significativement dans le groupe diltiazem (9,0 ± 2,8 L) comparé au groupe placebo (7,0 ± 1,6 L) mais ne différait pas dans le groupe dopamine (7,8 ± 1,8 L). Il n'y avait pas de différence significative intergroupe concernant la clairance de la créatinine. Tous les groupes montraient une augmentation d' α 1 -MG de T0 à T3, sans aucune différence significative entre les groupes. L' α-GST a augmenté de manière significative de T0 à T3 dans les groupes placebo (2,1 ± 1,8 à 11,4 ± 8,6 µg L -1 ) et d...

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Diltiazem may preserve renal tubular integrity after cardiac surgery

Piper

Kumle

Maleck

et al. 2003

Can J Anesth/J Can Anesth

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“…However, currently, none of these parameters for estimating warm ischemic damage and predicting transplant outcome is routinely used in clinical practice [5,6,171. One parameter, a-GST, had already been used in an evaluation study [8]. a-GST is found in the proximal tubulus [24], while n-GST is confined mainly to the distal tubulus [5,6,171.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that a strong correlation exists between WIT and the release of a-GST, and that a-GST can be used to define margins for the safe use of NHB donor kidneys. Post-transplant function was found to worsen with increasing (x-GST concentration [8]. However, the determination of a-GST concentration was done using an enzyme-linked immunosorbent assay (ELISA).…”

Section: Discussionmentioning

confidence: 99%

“…The appearance of a-GST in the perfusate of continuously perfused kidneys was found to predict the development of post-transplant acute tubule necrosis [8]. The aim of this study was to develop a rapid method of determining a-GST concentration.…”

Section: Acute Tubule Necrosis Proximal Tubule Damagementioning

confidence: 99%

“…All kidneys were from NHB donors and were preserved by machine perfusion at the University Hospital of Maastricht [8]. Samples for the determination of GST activity and a-GST concentration were taken from the perfusate at time points 0, 1, 2, 4, 6, and 8 h after preservation and immediately stored at -80 "C.…”

Section: Kidneysmentioning

confidence: 99%

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Functional relationship of alpha-glutathione S-transferase and glutathione S-transferase activity in machine-preserved non-heart-beating donor kidneys

Kreel¹,

Janssen²,

Kootstra³

2002

Transplant Int

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a-Glutathione S-transferase (a-GST) is a biochemical parameter used to estimate the amount of proximal tubule damage to a kidney. In normal clinical practice, the concentration of a-GST in urine is determined by a rather time-consuming immunochemical test, the enzyme-linked immunosorbent assay (ELISA). Kidneys from nonheart-beating (NHB) donors are perfused prior to transplantation. The determination of a-GST concentration in the perfusate to monitor damage is also done by means of an ELISA test. However, because this is a time-consuming method, it would be helpful to find a parameter proportional to GST concentrations that would be available within minutes. We therefore compared the method of determining a-GST concentration via ELISA with that of determining the enzymatic activity of GST, which is much faster (results available within 10 min). The comparison was made using 150 preserved kidneys that had been perfused for 6 h. The correlation was found to be very good, as indicated by the linear regression data: r = 0.954, P < 0.001. n-Glutathione S-transferase (n-GST) was also determined by means of an ELISA test, and the concentration of n-GST was compared with the enzymatic activity of the "total" GST. The precision of the enzymatic method, given by intra-and interassay variation, was 1.5% and 10.5%, respectively.

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Non-heart-beating Cadaver Donors

Kievit¹,

Nederstigt²,

Stubenitsky³

et al. 2001

Transplantation Surgery

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Glutathione S-Transferase as Predictor of Functional Outcome in Transplantation of Machine-Preserved Non-Heart-Beating Donor Kidneys

Cited by 119 publications

References 19 publications

RETRACTED ARTICLE: Diltiazem may preserve renal tubular integrity after cardiac surgery

RETRACTED ARTICLE: Diltiazem may preserve renal tubular integrity after cardiac surgery

Functional relationship of alpha-glutathione S-transferase and glutathione S-transferase activity in machine-preserved non-heart-beating donor kidneys

Non-heart-beating Cadaver Donors

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