Glutathione S-transferase activity in epithelial ovarian cancer: Association with response to chemotherapy and disease outcome

Ferrandina, Gabriella; Damia, Giovanna; Tagliabue, Giovanna; Fagotti, Anna; Panici, P. Benedetti; Mangioni, Costantino; Mancus, S.; D’Incalci, Maurizio

doi:10.1023/a:1008247428385

Cited by 36 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies clearly show an inverse correlation between GST-P expression and cancer. In studies of esophageal adenocarcinoma and ovarian cancer, the tumor tissue showed lower GST-P levels in tumor tissue than in the surrounding normal tissue (34,35). The current studies on canine lymphoma agree in that GST-P was observed more frequently in the normal lymph nodes.…”

Section: Discussionsupporting

confidence: 72%

Proteomics of Canine Lymphoma Identifies Potential Cancer-Specific Protein Markers

McCaw

Chan²,

Stegner³

et al. 2007

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: Early diagnosis of cancer is crucial for the success of treatment of the disease, and there is a need for markers whose differential expression between disease and normal tissue could be used as a diagnostic tool. Spontaneously occurring malignancies in pets provide a logical tool for translational research for human oncology. Lymphoma, one of the most common neoplasms in dogs, is similar to human non^Hodgkin's lymphoma and could serve as an experimental model system. Experimental Design: Thirteen lymph nodes from normal dogs and 11lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis. Results: A total of 93 differentially expressed spots was subjected to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis, and several proteins that showed differential expression were identified. Of these, prolidase (proline dipeptidase), triosephosphate isomerase, and glutathione S-transferase were down-regulated in lymphoma samples, whereas macrophage capping protein was up-regulated in the lymphoma samples. Conclusions: These proteins represent potential markers for the diagnosis of lymphoma and should be further investigated in human samples for validation of their utility as diagnostic markers.

show abstract

Section: Discussionsupporting

confidence: 72%

Proteomics of Canine Lymphoma Identifies Potential Cancer-Specific Protein Markers

McCaw

Chan²,

Stegner³

et al. 2007

Clinical Cancer Research

View full text Add to dashboard Cite

show abstract

“…For example, marked overexpression of GST P1-1 has been observed following the exposure of several tumor cell lines to various tumor drugs [5] and hence the enzyme is used as a biomarker. GST P1-1 is also overexpressed in CPT-resistant cell lines [10,11] and the inhibition of the enzyme has been shown to reverse resistance. [6] In addition, GST P1-1 knockout mice are considerably more sensitive to the cytotoxic effects of CPT treatment than their wild-type counterparts.…”

Section: Abstract: Antitumor Agents · a C H T U N G T R E N N U N G Dmentioning

confidence: 99%

Studies of Glutathione Transferase P1‐1 Bound to a Platinum(IV)‐Based Anticancer Compound Reveal the Molecular Basis of Its Activation

Parker

Italiano

Morton

et al. 2011

Chemistry A European J

View full text Add to dashboard Cite

Platinum-based cancer drugs, such as cisplatin, are highly effective chemotherapeutic agents used extensively for the treatment of solid tumors. However, their effectiveness is limited by drug resistance, which, in some cancers, has been associated with an overexpression of pi class glutathione S-transferase (GST P1-1), an important enzyme in the mercapturic acid detoxification pathway. Ethacraplatin (EA-CPT), a trans-Pt(IV) carboxylate complex containing ethacrynate ligands, was designed as a platinum cancer metallodrug that could also target cytosolic GST enzymes. We previously reported that EA-CPT was an excellent inhibitor of GST activity in live mammalian cells compared to either cisplatin or ethacrynic acid. In order to understand the nature of the drug-protein interactions between EA-CPT and GST P1-1, and to obtain mechanistic insights at a molecular level, structural and biochemical investigations were carried out, supported by molecular modeling analysis using quantum mechanical/molecular mechanical methods. The results suggest that EA-CPT preferentially docks at the dimer interface at GST P1-1 and subsequent interaction with the enzyme resulted in docking of the ethacrynate ligands at both active sites (in the H-sites), with the Pt moiety remaining bound at the dimer interface. The activation of the inhibitor by its target enzyme and covalent binding accounts for the strong and irreversible inhibition of enzymatic activity by the platinum complex.

show abstract

“…50,52 Studies that examine associations within specific histologic types of ovarian cancer are a promising area for further research.…”

Section: Disease Associationsmentioning

confidence: 99%

Glutathione S-transferase polymorphisms and risk of ovarian cancer: A HuGE review

Coughlin

Hall

2002

Genetics in Medicine

View full text Add to dashboard Cite

Glutathione S-transferases (GSTs) catalyze the conjugation of glutathione to numerous potentially genotoxic compounds. The GSTM1 gene codes for the enzyme glutathione S-transferase-mu, the GSTT1 gene codes for the enzyme glutathione S-transferase-theta, and the GSTP1 gene codes for the enzyme glutathione S-transferase-pi.GSTM1 is polymorphically expressed, and three alleles have been identified (GSTM1-0, GSTM1a, and GSTM1b).Two functionally different genotypes at the GSTT1 locus have been described. Individuals with homozygous deletions of GSTM or GSTT have reduced or no glutathione S-transferase activity and therefore may be unable to eliminate electrophilic carcinogens as efficiently. However, results of epidemiologic studies do not confirm associations between GSTM1, GSTT1, and GSTP1 and epithelial ovarian cancer. Genet Med 2002:4(4):250 -257.

show abstract

Glutathione S-transferase activity in epithelial ovarian cancer: Association with response to chemotherapy and disease outcome

Cited by 36 publications

References 25 publications

Proteomics of Canine Lymphoma Identifies Potential Cancer-Specific Protein Markers

Proteomics of Canine Lymphoma Identifies Potential Cancer-Specific Protein Markers

Studies of Glutathione Transferase P1‐1 Bound to a Platinum(IV)‐Based Anticancer Compound Reveal the Molecular Basis of Its Activation

Glutathione S-transferase polymorphisms and risk of ovarian cancer: A HuGE review

Contact Info

Product

Resources

About