Glutathione S-transferase activity in aquatic macrophytes and halophytes and biotransformation potential for biocides

Santos, Rodrigo Nunes Dos; Machado, Bruno Roswag; Hefler, Sônia Marisa; Zanette, Juliano

doi:10.1007/s10265-021-01266-8

Cited by 12 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the enzymatic activity was determined using a GST assay kit, based on a spectrophotometer with the general GST substrate CDNB. As a typical GST substrate, the CDNB was used to detect the GST in different organisms [ 35 ]. The results showed that both the R and S biotypes GST activity rise sharply, induced by the quinclorac within a certain period of time, and showed a trend of increasing first and then decreasing.…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis Comparison on the Ecological Adaptability of Quinclorac-Resistant Echinochloa crus-galli

Yang

et al. 2023

Plants

View full text Add to dashboard Cite

Barnyardgrass (Echinochloa crus-galli L.) is the most serious weed threatening rice production, and its effects are aggravated by resistance to the quinclorac herbicide in the Chinese rice fields. This study conducted a comparative proteomic characterization of the quinclorac-treated and non-treated resistant and susceptible E. crus-galli using isobaric tags for relative and absolute quantification (iTRAQ). The results indicated that the quinclorac-resistant E. crus-galli had weaker photosynthesis and a weaker capacity to mitigate abiotic stress, which suggested its lower environmental adaptability. Quinclorac treatment significantly increased the number and expression of the photosynthesis-related proteins in the resistant E. crus-galli and elevated its photosynthetic parameters, indicating a higher photosynthetic rate compared to those of the susceptible E. crus-galli. The improved adaptability of the resistant E. crus-galli to quinclorac stress could be attributed to the observed up-regulated expression of eight herbicide resistance-related proteins and the down-regulation of two proteins associated with abscisic acid biosynthesis. In addition, high photosynthetic parameters and low glutathione thiotransferase (GST) activity were observed in the quinclorac-resistant E. crus-galli compared with the susceptible biotype, which was consistent with the proteomic sequencing results. Overall, this study demonstrated that the resistant E. crus-galli enhanced its adaptability to quinclorac by improving the photosynthetic efficiency and GST activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis Comparison on the Ecological Adaptability of Quinclorac-Resistant Echinochloa crus-galli

Yang

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Moreover, these authors noted the role of glutathione in the atrazine detoxification process. Recently, dos Santos et al ( 2021 ) have demonstrated that macrophytes exhibiting greater GST activity also presented enhanced biotransformation potential against three different biocides (chlorothalonil, 4,5-dichloro- N -octyl-3(2H)-isothiazolone, and dichlofluanid). Furthermore, the free-floating macrophyte L. gibba also possesses similar detoxifying mechanisms involving glutathione (Alkimin et al 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Potential of Salvinia biloba Raddi for removing atrazine and carbendazim from aquatic environments

Loureiro

Lario

Herrero

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

In this exploratory study, naturally occurring Salvinia biloba Raddi specimens were assessed for atrazine and carbendazim polluted water remediation. Experiments were carried out over 21 days in glass vessels containing deionized water artificially contaminated with 0, 5, 10, and 20 mg L−1 of atrazine or carbendazim. Atrazine had a pronounced detrimental impact on S. biloba, as no biomass development was observed in all macrophytes exposed to this herbicide in the entire concentration range. However, carbendazim-treated plants were able to grow and survive in the polluted medium even when subjected to the highest concentration of this fungicide (i.e., 20 mg L−1). In addition, increased chlorosis and necrosis were also detected in plants subjected to carbendazim as a result of the high phytotoxicity caused by atrazine. A maximal removal efficiency of ~ 30% was observed for both pesticides at 5 mg L−1 and decreased with increasing concentrations of the pollutants. The spectrum of the FTIR-ATR analysis revealed the existence of various functional groups (e.g., amide, carboxyl, hydroxyl, phosphate, sulfate) on the plants, which could be related to pesticide biosorption. In addition, at the end of the 21-day assay, seven carbendazim-resistant bacteria could be isolated from the roots of fungicide-treated plants. Therefore, the use of autochthonous free-floating S. biloba macrophytes for phytoremediation of aquatic environments contaminated with carbendazim shows great promise. Still, additional research is required to further elucidate the plant-mediated carbendazim elimination process and the role of the herbicide-resistant bacteria, and seek alternative species capable of mitigating atrazine contamination.

show abstract

“…MELO3C020906 encodes 60S ribosomal protein L26‐1‐like and is involved in ribosome biogenesis and function (Babiano et al., 2012), while MELO3C003554 encoding homeobox‐leucine zipper protein ATHB‐6‐like has been linked with drought tolerance in plants (Jiao et al., 2022). Other genes included MELO3C021416 , which encodes zinc finger A20 and AN1 domain‐containing stress‐associated protein 3, likely associated with the plant's response to abiotic stress (Rania et al., 2018) and MELO3C001175 , encoding glutathione S‐transferase U9‐like, a GST (lutathione S‐transferase) responsible for phytoremediation (Dos Santos et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Other genes included MELO3C021416, which encodes zinc finger A20 and AN1 domain-containing stress-associated protein 3, likely associated with the plant's response to abiotic stress (Rania et al, 2018) and MELO3C001175, encoding glutathione S-transferase U9-like, a GST (lutathione Stransferase) responsible for phytoremediation (Dos Santos et al, 2021).…”

Section: Analysis Of Degs and Pathways Induced By Silver Nitrate Trea...mentioning

confidence: 99%

Identification of key genes promoting stamen formation induced by silver nitrate in gynoecious melon

Dai,

Kexin,

Liu

et al. 2023

Crop Science

View full text Add to dashboard Cite

The gynoecious melon shows a high degree of hybrid seed production advantage and exploitation of this property could improve both seed production and breeding, as well as reduce labor costs in melon production. The mechanism responsible the formation of hermaphroditic flowers by exogenous AgNO3 spraying is unclear. In the present study, the gynoecious inbred melon line WI998 was treated with AgNO3 solution to produce hermaphrodite flowers. Seedlings were investigated at the two, three, four, and five true‐leaf stages, and the AgNO3 concentrations were 150, 300, 450, and 600 mg/L. We found that the optimum spray concentration of AgNO3 was 450 mg/L with an optimum application time at the four true leave‐leaf stage. Transcriptome sequencing was performed on four‐true‐leaf stage samples sprayed with 450 mg/L AgNO3 and water control to identify differentially expressed genes Twenty‐seven differentially expressed genes were identified and one, Cucumis melo Epidermal Pattern Factor‐Like Protein 4 (CmEPFL4), was involved in stamen development gene. Phenotypic analysis showed that CmEPFL4 played a key role in stamen induction, and heterologous expression of CmEPFL4 was found to interfere with stamen development and reduce the amount of pollen produced. Overall, the results demonstrated the importance of CmEPFL4 in AgNO3‐induced hermaphroditic flower production and provide insight into AgNO3 induction of stamen formation in the melon.This article is protected by copyright. All rights reserved

show abstract

Glutathione S-transferase activity in aquatic macrophytes and halophytes and biotransformation potential for biocides

Cited by 12 publications

References 34 publications

Proteomic Analysis Comparison on the Ecological Adaptability of Quinclorac-Resistant Echinochloa crus-galli

Proteomic Analysis Comparison on the Ecological Adaptability of Quinclorac-Resistant Echinochloa crus-galli

Potential of Salvinia biloba Raddi for removing atrazine and carbendazim from aquatic environments

Identification of key genes promoting stamen formation induced by silver nitrate in gynoecious melon

Contact Info

Product

Resources

About