Glutathione S-transferase A1, M1, P1 and T1 null or low-activity genotypes are associated with enhanced oxidative damage among haemodialysis patients

Šuvakov, Sonja; Damjanović, Tatjana; Stefanović, Aleksandra; Pekmezović, Tatjana; Savić-Radojević, Ana; Plješa-Ercegovac, Marija; Matić, Marija; Djukić, Tatjana; Ćorić, Vesna; Jakovljevic, Jovana; Ivanišević, Jasmina; Pljesa, Steva; Jelić‐Ivanović, Zorana; Mimic-Oka, Jasmina; Dimković, Nada; Simić, Tatjana

doi:10.1093/ndt/gfs369

Cited by 57 publications

(66 citation statements)

References 53 publications

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“…The genotype distribution as well as the frequency of the Ile105Val polymorphism observed in this study did not largely differ from those reported in another Central European population, the Czechs; the variant allele in this group has a reported frequency of 34.4%, and the heterozygote genotype was found to be associated with a decreased risk of colorectal cancer (Hezova et al, 2012). In contrast to our results, the frequency of this allele was higher in a Serbian population, reaching 37.6% (Suvakov et al, 2013). The genotype Ile105Ile frequency observed in our study (41.8%) was similar to that determined by Vichi et al (2012) in a healthy Italian population (42%), where this genotype was found to be associated with a higher risk of endometriosis (frequency in cases, 48.6%).…”

Section: Discussioncontrasting

confidence: 99%

“…In the 1.67-kb promoter region (with exon 1 fragment) of the human GSTA1 gene, we identified 8 SNPs, including G-52A, C-69T, A-513G, T-567G, G-631T, A-1066T, G-1142C, and G-1245A, confirming the results of Bredschneider et al (2002) in Caucasians, except for the SNP that was only observed in our study at site -1245. The frequency of the GSTA1*B allele containing 3 linked basic variants affecting gene expression (-52A, -69T, -567G) observed in our study among 160 individuals (43.1%) was slightly lower than that observed by Bredschneider et al (2002) among 48 subjects (47.9%), but showed some similarities, including genotype distribution, to the data of global studies in the Caucasian population (38-50%) (Coles et al, 2001;Spalletta et al, 2012;Suvakov et al, 2013). However, in African-American, Japanese, and Chinese subjects, the allele frequency was much lower, at 26, 16, and 12.9%, respectively (Coles et al, 2001;Matsuno et al, 2004;Ping et al, 2006).…”

Section: Discussionsupporting

confidence: 60%

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Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population

et al. 2015

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 60%

Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population

et al. 2015

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“…GSTM1 and GSTT1 polymorphism were identified by PCR, while GSTA1 and GSTP1 were identified by restriction fragment length polymorphism PCR (RFLP-PCR) method as previously described (Suvakov et al 2013). …”

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

“…It has been shown that polymorphic expression of GSTs is associated with the altered xenobiotics detoxification efficiency, thus influencing an individual's susceptibility to carcinogens and various diseases (Di Pietro et al 2010;Suvakov et al 2013;Eslami and Sahebkar 2014;Kim et al 2015). Still, there are no data regarding the association between GST genetic polymorphism and bladder cancer risk, neither in patients with BEN, nor in individuals who originate from BEN regions.…”

Section: Introductionmentioning

confidence: 99%

Common Polymorphisms in GSTA1, GSTM1 and GSTT1 Are Associated with Susceptibility to Urinary Bladder Cancer in Individuals from Balkan Endemic Nephropathy Areas of Serbia

Matić

Dragićević²,

Pekmezović

et al. 2016

Tohoku J. Exp. Med.

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Balkan endemic nephropathy (BEN) is a chronic familial form of interstitial nephritis that might eventually lead to end stage renal disease. This nephropathy affects individuals living along of the Danube River and its tributaries in Serbia, Bosnia, Croatia, Bulgaria and Romania. The increased incidence of urinary tract tumors in the BEN areas is well described, but its specific genetic predisposition is still unclear. Certain nephrocarcinogenic compounds, including those associated with BEN, are metabolized by glutathione S-transferase (GST) superfamily of phase II detoxication enzymes. Importantly, the GST-mediated detoxification may result in formation of more toxic compounds. We examined the association of common GST polymorphisms and bladder cancer (BC) risk in individuals from BEN areas in Serbia. A hospitalbased case-control study included 201 BC cases (67 from BEN region) and 122 controls. Each polymorphism was identified by a PCR-based method. Individuals from BEN region with low-expression GSTA1 genotype (AB+BB) exhibited a 2.6-fold higher BC risk compared to those with GSTA1 (AA) genotype who were from non-BEN region (OR = 2.60, p = 0.015). In contrast, carriers of GSTM1-active genotype from BEN region had a 2.9-fold increased BC risk compared to those with GSTM1-active genotype from non-BEN region (OR = 2.90, p = 0.010). Likewise, carriers with GSTT1-active genotype from BEN region exhibited 2.1-fold higher BC risk compared to those from non-BEN region with GSTT1-active genotype (OR = 2.10, p = 0.027). Thus, common polymorphisms in GSTA1, GSTM1 and GSTT1 are associated with susceptibility to BC in individuals from BEN areas of Serbia.

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“…Furthermore, GSTA1 serves as a crucial role in the GSH binding reaction. In normal liver and kidney tissues, GSTA1-encoded dimeric protein expression is high and serves an important role in the anti-oxidative defense system (14). It is able to catalyze many xenobiotics, such as carcinogens, environmental toxins and certain pharmacological agents, by combining with GSH to promote the degradation of these substances (15).…”

Section: Introductionmentioning

confidence: 99%

Expression of glutathione S-transferase A1, a phase II drug-metabolizing enzyme in acute hepatic injury on mice

Liu

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In the present study, three models of acute liver injury in mice were induced via the administration of CCl 4 (35 mg/kg, 24 h), acetyl-para-aminophenol (APAP; 200 mg/kg, 12 h) and ethanol (14 ml/kg, 8 h) to study the effect of glutathione S-transferase A1 (GSTA1) on acute liver injury. The serum levels of alanine transaminase, aspartate transaminase and liver homogenate indicators (superoxide dismutase, glutathione and glutathione peroxidase) were significantly lower in model groups compared with the control group (P<0.01), whereas the liver homogenate indicator malondialdehyde was significantly increased (P<0.01). The expression of GSTA1 in liver was significantly decreased in the model groups compared with the control group (P<0.01). GSTA1 protein content was 3.8, 1.3 and 2.6 times lower in the CCl 4, APAP and ethanol model groups, respectively. Furthermore, GSTA1 mRNA expression levels decreased by 4.9, 2.1 and 3.7 times in the CCl 4, APAP and ethanol model groups, respectively. Among the three models, the injury induced by CCl 4 was the most marked, followed by ethanol and finally APAP. These results suggest that GSTA1 may be released by the liver and serve as an antioxidant in the prevention of liver damage. IntroductionLiver disease poses a serious threat to human health and food safety, as consumption of animals with liver disease may be detrimental to health (1). Acute liver injury is the common pathway and initiating factor of many liver diseases, such as acute liver failure (2). Three models of liver injury are typically used in research as they are representational and reflect the situation of hepatotoxicity comprehensively and intuitively (3). CCl 4 is a classical hepatotoxicant, which is able to induce reactive oxygen formation and deplete glutathione (GSH) (4). Acetyl-para-aminophenol (APAP) hepatotoxicity is induced by the electrophile N-acetyl-p-benzoquinoneimine (NAPQI), which is able to induce mitochondrial dysfunction and oxidative stress, leading to liver damage (5). The major etiological factors of hepatotoxicity in ethanol-induced hepatic injury are oxidative stress and inflammatory responses (6). Due to the complexity of liver function and the diversity of liver damage factors, experimental animal models are not able to accurately and fully reflect the nature of liver injury (7). Furthermore, existing animal models have various limitations such as non-standardized methods, lack of reproducibility and non-unified methods (8).Glutathione S-transferases (GSTs) are enzymes that are able to protect cells from damage caused by reactive oxygen species (9). GSTA (α class GST) serves an important cytoprotective role in detoxifying reactive electrophiles and products of lipid peroxidation (10). GSTs including GSTA have previously been identified as inhibitors of stress-activated kinase activity, most notably c-Jun N-terminal kinase (11). This suggests that altered GST expression may be an important factor in modulating the cellular transition between proliferation, differentiation, and apo...

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Glutathione S-transferase A1, M1, P1 and T1 null or low-activity genotypes are associated with enhanced oxidative damage among haemodialysis patients

Abstract: According to the GST genotype, ESRD patients may be stratified in terms of the level of oxidative and carbonyl stress that might influence cardiovascular prognosis, but could also improve efforts towards individualization of antioxidant treatment.

Cited by 57 publications

References 53 publications

Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population

Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population

Common Polymorphisms in<i> GSTA1</i>, <i>GSTM1</i> and <i>GSTT1 </i>Are Associated with Susceptibility to Urinary Bladder Cancer in Individuals from Balkan Endemic Nephropathy Areas of Serbia

Expression of glutathione S-transferase A1, a phase II drug-metabolizing enzyme in acute hepatic injury on mice

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