Glutathione Reductase from Oryza sativa Increases Acquired Tolerance to Abiotic Stresses in a Genetically Modified Saccharomyces cerevisiae Strain

Kim, Il-Sup; Kim, Young-Saeng; Yoon, Ho-Sung

doi:10.4014/jmb.1202.02028

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…When the toxic metals enter the fungal spores, they associate with the particulate insoluble cytoplasmic components and react with cytoplasmic receptor sites and caused inhibition of spore germination (Nazareth and Marbaniang, 2008). Fundamentally, the exposure to metal stress induced structural changes associated with the type of metal and its concentration (Kim et al, 2012;Luna et al, 2015). The morphological changes may be essential for metal removal (Li et al, 2017;Gururajan and Belur, 2018).…”

Section: Sem Edx and Tem Analysesmentioning

confidence: 99%

Tolerance and mycoremediation of silver ions by Fusarium solani

Sayed

El-Sayed

2020

Heliyon

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Silver ions discharged from various industries, are potentially toxic to living organisms at low concentrations, thus, there is an increasing need for development of an eco-friendly and cost-effective approach for its bioremediation. Filamentous fungi especially, Fusarium solani displayed a strong resistance to copper and cadmium ions as revealed from our previous study (El-Sayed 2014), however, the mechanisms of silver resistance by this fungus has not been resolved yet. Thus, this study was an extension to our previous work, to elucidate the mechanism of silver ions resistance and biotransformation by F. solani. The growth, bioaccumulation, thiol, total antioxidant, malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) contents and polyphenol oxidase (PPO) and catalase (CAT) activities of F. solani in response to silver ions were determined. Production and bioaccumulation of silver nanoparticles was characterized by UV-visible spectroscopy, TEM, and X-ray powder diffraction (XRD). The ultrastructural changes of F. solani induced by Ag(I) was examined by TEM and SEM. Production of oxalic acid by F. solani was increased by about 343.8% in response to 400 mg/l Ag(I), compared to control cultures (without silver ions) as revealed from HPLC analysis. The maximum biosorption levels by the native and alkali-treated biomass were carried out at pH 5.0, initial metal concentration 200 mg/l, biomass 0.5 g/l, temperature 35 C, and contact time 1 h (native biomass) and 3 h (alkali-treated biomass). Fourier transform infrared spectroscopy (FTIR) results revealed that the main functional groups involved on this mycoremediation were C-S stretching, C¼O C¼N, C -H bending, C-N stretching and N-H bending. EDX spectra indicated the involvement of fungal cellular sulfur and phosphorus compounds in Ag(I) binding.

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Section: Sem Edx and Tem Analysesmentioning

confidence: 99%

Tolerance and mycoremediation of silver ions by Fusarium solani

Sayed

El-Sayed

2020

Heliyon

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“…This provides a reducing intracellular environment, which is thought to retain the oxidation-sensitive thiol groups of cysteine residues of proteins in a reduced state [17]. Overexpression of glutathione reductase from Oryza sativa and Brassica rapa in S. cerevisiae has recently been shown to increase tolerance against oxidative stress induced by H 2 O 2 and abiotic stresses such as heavy metals [18,19]. …”

Section: Introductionmentioning

confidence: 99%

Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases robustness to inhibitors in pretreated lignocellulosic materials

et al. 2013

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BackgroundProduction of bioethanol from lignocellulosic biomass requires the development of robust microorganisms that can tolerate the stressful conditions prevailing in lignocellulosic hydrolysates. Several inhibitors are known to affect the redox metabolism of cells. In this study, Saccharomyces cerevisiae was engineered for increased robustness by modulating the redox state through overexpression of GSH1, CYS3 and GLR1, three genes involved in glutathione (GSH) metabolism.ResultsOverexpression constructs were stably integrated into the genome of the host strains yielding five strains overexpressing GSH1, GSH1/CYS3, GLR1, GSH1/GLR1 and GSH1/CYS3/GLR1. Overexpression of GSH1 resulted in a 42% increase in the total intracellular glutathione levels compared to the wild type. Overexpression of GSH1/CYS3, GSH1/GLR1 and GSH1/CYS3/GLR1 all resulted in equal or less intracellular glutathione concentrations than overexpression of only GSH1, although higher than the wild type. GLR1 overexpression resulted in similar total glutathione levels as the wild type. Surprisingly, all recombinant strains had a lower [reduced glutathione]:[oxidized glutathione] ratio (ranging from 32–67) than the wild type strain (88), suggesting a more oxidized intracellular environment in the engineered strains. When considering the glutathione half-cell redox potential (Ehc), the difference between the strains was less pronounced. Ehc for the recombinant strains ranged from -225 to -216 mV, whereas for the wild type it was estimated to -225 mV. To test whether the recombinant strains were more robust in industrially relevant conditions, they were evaluated in simultaneous saccharification and fermentation (SSF) of pretreated spruce. All strains carrying the GSH1 overexpression construct performed better than the wild type in terms of ethanol yield and conversion of furfural and HMF. The strain overexpressing GSH1/GLR1 produced 14.0 g L-1 ethanol in 48 hours corresponding to an ethanol yield on hexoses of 0.17 g g-1; while the wild type produced 8.2 g L-1 ethanol in 48 hours resulting in an ethanol yield on hexoses of 0.10 g g-1.ConclusionsIn this study, we showed that engineering of the redox state by modulating the levels of intracellular glutathione results in increased robustness of S. cerevisiae in SSF of pretreated spruce.

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“…The homology models of representative GRs from Rice (LOC_Os02 g56850), Arabidopsis (AT3G24170) were created using ExPasy (OsGR) in yeast shows tolerance against various abiotic stresses. 13 Overall the present study would be helpful to our better understanding of the GR under normal and stress condition in plants.…”

Section: Resultsmentioning

confidence: 77%

Genome-wide analysis of glutathione reductase (GR) genes from rice and Arabidopsis

Trivedi¹,

Gill²,

Yadav³

et al. 2013

Plant Signaling & Behavior

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Plant cells and tissues remain always on risk under abiotic and biotic stresses due to increased production of reactive oxygen species (ROS). Plants protect themselves against ROS induced oxidative damage by the upregulation of antioxidant machinery. Out of many components of antioxidant machinery, glutathione reductase (GR, EC 1.6.4.2) and glutathione (GSH, γ-Glu-Cys-Gly) play important role in the protection of cell against oxidative damage. In stress condition, the GR helps in maintaining the reduced glutathione pool for strengthening the antioxidative processes in plants. Present study investigates genome wide analysis of GR from rice and Arabidopsis. We were able to identify 3 rice GR genes (LOC_Os02 g56850, LOC_Os03 g06740, LOC_Os10 g28000) and 2 Arabidopsis GR genes (AT3G54660, AT3G24170) from their respective genomes on the basis of their annotation as well as the presence of pyridine nucleotide-disulphide oxidoreductases class-I active site. The evolutionary relationship of the GR genes from rice and Arabidopsis genomes was analyzed using the multiple sequence alignment and phylogenetic tree. This revealed evolutionary conserved pyridine nucleotide-disulphide oxidoreductases class-I active site among the GR protein in rice and Arabidopsis. This study should make an important contribution to our better understanding of the GR under normal and stress condition in plants.

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Glutathione Reductase from Oryza sativa Increases Acquired Tolerance to Abiotic Stresses in a Genetically Modified Saccharomyces cerevisiae Strain

Cited by 12 publications

References 32 publications

Tolerance and mycoremediation of silver ions by Fusarium solani

Tolerance and mycoremediation of silver ions by Fusarium solani

Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases robustness to inhibitors in pretreated lignocellulosic materials

Genome-wide analysis of glutathione reductase (GR) genes from rice and Arabidopsis

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