Glutathione, protein sulfhydryls and cysteine proteases in gastric mucosal injury and protection

Szabó, Sándor; Nagy, Lajos; Plebani, Mario

doi:10.1016/0009-8981(92)90010-n

Cited by 96 publications

(75 citation statements)

References 20 publications

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“…GSH provides major protection in oxidative injury by participating in the cellular defense against oxidative damage and protects protein thiol groups from oxidation, 55 while depletion of tissue GSH is one of the primary factors that permit lipid peroxidation to occur. 56 In the present study, tissue GSH levels were depleted in CRF animals. However, the AGE treatment, which ameliorated oxidative damage and cytokine levels, did not replenish GSH levels.…”

Section: Discussionsupporting

confidence: 52%

Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure

et al. 2011

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Chronic renal failure (CRF) is associated with oxidative stress that promotes production of reactive oxygen species and cytokine release. We aimed to investigate the possible protective and antioxidant effects of aqueous garlic extract (AGE) in a rat model of CRF. Male Sprague-Dawley rats were randomly assigned as either CRF group with 5/6 reduction in the renal mass or sham-operated control group. CRF group received either saline or AGE (250 mg/kg/day/1 mL) orally for 3 weeks. At the end of the 3 weeks, rats were decapitated and trunk blood was collected. Creatinine, blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) activity, and TNF-α and IL-1β levels were measured in the serum samples, while malondialdehyde (MDA), glutathione (GSH) levels, and myeloperoxidase (MPO) activity were determined in the kidney, lung, and heart samples. CRF caused significant decreases in tissue GSH, which were accompanied with significant increases in MDA levels and MPO activities, while the circulating levels of the LDH activity, creatinine, BUN, TNF-α, and IL-1β were elevated. AGE treatment alleviated CRF-induced oxidative changes in the injured tissues, while CRF-induced elevations in the blood levels of the pro-inflammatory cytokines and LDH were reduced. In conclusion, CRF-induced oxidative tissue injury occurs via the activation of pro-inflammatory mediators and by neutrophil infiltration into tissues and that the protective effects of garlic on CRF-induced injury can be attributed to its ability to inhibit neutrophil infiltration and pro-inflammatory mediators. These findings suggest that garlic, as a supplementary to diet, may have a potential therapeutic use in delimitating the systemic oxidant effects of CRF on remote organs.

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Section: Discussionsupporting

confidence: 52%

Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure

et al. 2011

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“…The role of glutathione as a protective agent against oxidative organ damage has been subject to extensive studies [31]. The exposed sulfhydryl groups bind to a variety of electrophilic radicals and metabolites that may cause cell damage [32]. Detoxification of xenobiotics or their metabolites is one of the major functions of glutathione.…”

Section: Resultsmentioning

confidence: 99%

Effect of Aquilegia vulgaris (L.) ethyl ether extract on liver antioxidant defense system in rats

Ewertowska¹,

Jodynis‐Liebert²,

Kujawska³

et al. 2009

International Journal of Occupational Medicine and Environmental Health

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Introduction:The ethyl ether extract from Aquilegia vulgaris (L.) (Ranunculaceae) contains a lot of phenolic acids. Their hydroxyl groups are capable of donating hydrogen atoms at the initial stage of lipid peroxidation (LPO), which inactivates hydroxyperoxides formed from polyunsaturated fatty acids (PUFAs) and leads to breakdown of the propagation chain. Material and methods: Rats pretreated with acetaminophen (APAP) (600 mg/kg b.w., p.o.) were given ethyl ether extract (100 mg/kg b.w., p.o.) obtained from A. vulgaris herb. The study parameters measured were microsomal lipid peroxidation, reduced glutathione, and the activity of hepatic antioxidant enzymes and some drug metabolizing enzymes. Results:The treatment with ethyl ether extract of the herb produced a 87-95% decrease in uninduced and Fe 2+ /ascorbate-stimulated microsomal lipid peroxidation in the liver of rats receiving APAP. Hepatic glutathione level depleted by APAP increased significantly (by 18%) after the extract treatment. Antioxidant enzyme activity in the liver, inhibited by APAP, was found to increase after administration of the extract: catalase by about 36%, glutathione reductase by 27% and glutathione S-transferase by 29%. Glucose-6-phosphate dehydrogenase, which decreased after APAP administration, increased again by 26% after extract treatment. The extract tested did not affect the activity of DT-diaphorase. The cytochrome P450 content, depleted by APAP, increased as much as by 100% after the treatment. The activities of NADPH-cytochrome P450 reductase, aniline hydroxylase and aminopyrine N-demethylase were not affected. Conclusions: The protective effect of the Aquilegia vulgaris extract in APAP-induced liver injury was mediated by its antioxidant activity. The extract did not inhibit the formation of reactive intermediate metabolites of APAP.

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“…Protein and non-protein sulfhydryls were suggested also as endogenous protective compounds [29,30], and it was raised that the maintenance of a critical level of non-protein sulfhydryls in the gastric mucosa is important for the gastroprotective action -besides nitric oxide [31].…”

Section: Protein and Non-protein Sulfhydrylsmentioning

confidence: 99%

The Role of Capsaicin-Sensitive Afferent Nerves in Gastric Mucosal Protection Initiated Centrally or Peripherally under Experimental Conditions

Gyires¹

2014

Capsaicin - Sensitive Neural Afferentation and the Gastrointestinal Tract: From Bench to Bedside

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Glutathione, protein sulfhydryls and cysteine proteases in gastric mucosal injury and protection

Cited by 96 publications

References 20 publications

Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure

Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure

Effect of Aquilegia vulgaris (L.) ethyl ether extract on liver antioxidant defense system in rats

The Role of Capsaicin-Sensitive Afferent Nerves in Gastric Mucosal Protection Initiated Centrally or Peripherally under Experimental Conditions

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