Glutathione Peroxidases in Plants: Innumerable Role in Abiotic Stress Tolerance and Plant Development

Madhu,; Sharma, Alok; Kaur, Amandeep; Tyagi, Shivi; Upadhyay, Santosh Kumar

doi:10.1007/s00344-022-10601-9

Cited by 38 publications

(10 citation statements)

References 112 publications

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“…Plant genomes encode between two ( Panax ginseng ) and 14 (wheat) GPXL‐isoforms (Madhu et al, 2023). Among the eight isoforms in A .…”

Section: Discussionmentioning

confidence: 99%

Regeneration of cytosolic thiol peroxidases

Vogelsang,

Dietz

2023

Physiologia Plantarum

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Three soluble type two peroxiredoxins (PRXIIB, C, D) and two glutathione peroxidase‐like enzymes (GPXL2, 8) reside in the cytosol of Arabidopsis thaliana cells and function both as thiol‐dependent antioxidants and redox sensors. Their primary substrate is H2O2, but they also accept other peroxides with a distinct preference between PRXII and GPXL. Less known is their regeneration specificity in the light of the large set of thiol reductases, namely eight annotated thioredoxin h isoforms (TRXh1‐5, 7–9), a few TRX‐like proteins, including CxxS1 (formerly TRXh6) and several glutaredoxins (GRX) associated with the cytosol. This study addressed this open question by in vitro enzyme tests using recombinant protein. GPXL2 and 8 exclusively accepted electrons from the TRX system, namely TRXh1‐5 and TDX, while PRXIIB/C/D were efficiently regenerated with GRXC1 and C2 but not the TRX‐like protein Picot1. They showed significant but low activity (<3% of GRXC2) with TRXh1‐5 and TDX. A similar reduction efficiency with TRX was seen in the insulin assay, only TDX was less active. Finally, the reduction of oxidized cytosolic malate dehydrogenase 1, as measured by regained activity, showed an extremely broad ability to accept electrons from different TRXs and GRXs. The results demonstrate redundancy and specificity in the redox regulatory network of the cytosol.

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“…Plant genomes encode between two ( Panax ginseng ) and 14 (wheat) GPXL‐isoforms (Madhu et al, 2023). Among the eight isoforms in A .…”

Section: Discussionmentioning

confidence: 99%

Regeneration of cytosolic thiol peroxidases

Vogelsang,

Dietz

2023

Physiologia Plantarum

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“…The BLASTp search approach at the Ensembl Plant database was used to scan the T. aestivum genome for the identification of TaTPKs using the known TPK sequences of A. thaliana and O. sativa at e-value e −10 as done in earlier studies [ [29] , [30] , [31] ]. The HMMER and Pfam database searches were also carried out to validate the identification of retrieved sequences through domain confirmation [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Investigation of two-pore K+ (TPK) channels in Triticum aestivum L. suggests their role in stress response

Kaur,

Madhu,

Sharma

et al. 2024

Heliyon

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“…Plants possess almost a limitless ability to synthesize a wide variety of secondary metabolic compounds in different organs like leaves, shoots, roots, and flowers via different biochemical pathways, which protect themselves against biotic and abiotic stress ( Wang et al., 2022 ; Sharma et al., 2023 ). These aromatic compounds, like tannins, terpenoids, alkaloids, flavonoids, phenols or their oxygen-substituted derivatives, are extensively being used as agrochemicals, dyes, insecticides, food additives and pharmaceuticals ( Gantait and Mukherjee, 2021 ; Sharma et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. ‘Hairy roots’, mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the in vitro production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with Agrobacterium rhizogenes. With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.

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Glutathione Peroxidases in Plants: Innumerable Role in Abiotic Stress Tolerance and Plant Development

Cited by 38 publications

References 112 publications

Regeneration of cytosolic thiol peroxidases

Regeneration of cytosolic thiol peroxidases

Investigation of two-pore K+ (TPK) channels in Triticum aestivum L. suggests their role in stress response

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

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