Glutathione peroxidase activity in seminal plasma and its relationship to classical sperm parameters and in vitro fertilization-intracytoplasmic sperm injection outcome

Crisol, Lorena; Matorras, Roberto; Aspichueta, F.; Expósito, Antonia; Hernández, M. Luisa; Ruiz‐Larrea, M. Begoña; Mendoza, Rosario; Ruiz-Sanz, José Ignacio

doi:10.1016/j.fertnstert.2012.01.097

Cited by 30 publications

(21 citation statements)

References 32 publications

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“…However, in pathological infertile subjects, the only correlation between GSH-Px activity and sperm parameters ( Figure 2) is observed in the group with a low sperm count (OTS), where GSH-Px activity was positively correlated with sperm morphology (r = 0.8146, P = 0.0075). This is in accordance with previous findings described by Foresta et al (2002) and Crisol et al (2012). Nevertheless, the activity of GSH-Px was significantly decreased in all infertile groups with sperm pathologies related to NS ( Figure 3A), indicating that GSH-Px activity in seminal plasma could make difference between infertile males with normal and subnormal semen characteristics (TS, OTS, and OATS).…”

Section: Resultssupporting

confidence: 92%

Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis

Otašević

Kalezic

Macanovic

et al. 2019

Systems Biology in Reproductive Medicine

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Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals\. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm parameters, as well as their potential to serve as biomarkers of specific sperm pathologies. Although the activity of CuZnSOD and protein expression of catalase were significantly correlated with several sperm parameters, underlying their potential role in etiology of various sperm abnormalities, investigation of their potential usefulness as a biomarker of semen quality showed that these AD enzymes could not distinguish subtle differences between various sperm pathologies. In contrast, GSH-Px activity was decreased in all groups with sperm pathologies and was a very good indicator of aberrations in functional sperm parameters, explaining up to 94.6% of infertility cases where functional sperm parameters were affected. Therefore, assessment of GSH-Px activity showed the potential to discriminate between infertile males with normal and subnormal semen characteristics and may prove useful in the evaluation of male (in)fertility.

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Section: Resultssupporting

confidence: 92%

Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis

Otašević

Kalezic

Macanovic

et al. 2019

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…[40] GSH peroxidase activity was reduced in individuals diagnosed with severe asthenozoospermia, oligozoospermia, and teratozoospermia. [41]…”

Section: Antioxidants In Semenmentioning

confidence: 99%

Role of oxidative stress in male infertility: An updated review

2019

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Current evidence links oxidative stress (OS) to male infertility, reduced sperm motility, sperm DNA damage and increased risk of recurrent abortions and genetic diseases. A review of PubMed, Medline, Google Scholar, and Cochrane review databases of published articles from years 2000–2018 was performed focusing on physiological and pathological consequences of reactive oxygen species (ROS), sperm DNA damage, OS tests, and the association between OS and male infertility, pregnancy and assisted reproductive techniques outcomes. Generation of ROS is essential for reproductive function, but OS is detrimental to fertility, pregnancy, and genetic status of the newborns. Further, there is a lack of consensus on selecting OS test, type, and duration of antioxidants treatment as well as on the target patients group. Developing advanced diagnostic and therapeutic options for OS is essential to improve fertility potential and limit genetic diseases transmitted to offspring.

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“…All GPx isozymes reduce hydrogen peroxide and lipid hydroxyperoxides [ 147 , 148 ]. Seminal GPx activity is lower in infertile men with abnormal spermatozoa quality, but no correlation was found between GPx levels and spermatozoa fertilization potential or pregnancy rates in IVF [ 149 ].…”

Section: Antioxidants As Ameliorating Agentsmentioning

confidence: 99%

Utility of antioxidants during assisted reproductive techniques: an evidence based review

Agarwal

Durairajanayagam

Plessis

2014

Reprod Biol Endocrinol

178

134

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Assisted reproductive technology (ART) is a common treatment of choice for many couples facing infertility issues, be it due to male or female factor, or idiopathic. Employment of ART techniques, however, come with its own challenges as the in vitro environment is not nearly as ideal as the in vivo environment, where reactive oxygen species (ROS) build-up leading to oxidative stress is kept in check by the endogenous antioxidants system. While physiological amounts of ROS are necessary for normal reproductive function in vivo, in vitro manipulation of gametes and embryos exposes these cells to excessive ROS production either by endogenous or exogenous environmental factors. In this review, we discuss the sources of ROS in an in vitro clinical setting and the influence of oxidative stress on gamete/embryo quality and the outcome of IVF/ICSI. Sources of ROS and different strategies of overcoming the excessive generation of ROS in vitro are also highlighted. Endogenously, the gametes and the developing embryo become sources of ROS. Multiple exogenous factors act as potential sources of ROS, including exposure to visible light, composition of culture media, pH and temperature, oxygen concentration, centrifugation during spermatozoa preparation, ART technique involving handling of gamete/embryo and cryopreservation technique (freeze/thawing process). Finally, the use of antioxidants as agents to minimize ROS generation in the in vitro environment and as oral therapy is highlighted. Both enzymatic and non-enzymatic antioxidants are discussed and the outcome of studies using these antioxidants as oral therapy in the male or female or its use in vitro in media is presented. While results of studies using certain antioxidant agents are promising, the current body of evidence as a whole suggests the need for further well-designed and larger scale randomized controlled studies, as well as research to minimize oxidative stress conditions in the clinical ART setting.

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Glutathione peroxidase activity in seminal plasma and its relationship to classical sperm parameters and in vitro fertilization-intracytoplasmic sperm injection outcome

Cited by 30 publications

References 32 publications

Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis

Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis

Role of oxidative stress in male infertility: An updated review

Utility of antioxidants during assisted reproductive techniques: an evidence based review

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