Glutathione peroxidase activity in erythrocytes and muscle of cattle and sheep and its relationship to selenium

Anderson, Peter H.; Berrett, Sylvia; Patterson, D.S.P.

doi:10.1016/0021-9975(78)90022-1

Cited by 68 publications

(18 citation statements)

References 19 publications

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“…Creatine kinase was determined using the Bayer kit no. T01-1882-85 on a Bayer Technicon RA2000 analyser and glutathione peroxidase determined according to Anderson et al (1978) using a Bayer Technicon RA-XT analyser.…”

Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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In order to determine the effect of dietary vitamin E level and basal diet on vitamin E status, performance and tissue fatty acid content, five groups of eight Suffolk 3 Charollais wether lambs with an initial live weight of 28.4 (s.d. 1.6) kg were allocated to one of five concentrate-based diets supplemented with all-rac-a-tocopheryl acetate to contain 30 mg (C-30), 60 mg (C-60), 120 mg (C-120), 250 mg (C-250) or 500 mg (C-500) a-tocopheryl acetate/kg dry matter (DM), for 63 days. Two additional groups of eight lambs entered the study at 31.2 (s.d. 3.3) kg and were fed grass silage and 400 g/day concentrate for 56 days, with the whole diet providing the equivalent of 60 mg (S-60) or 500 mg (S-500) a-tocopheryl acetate/kg DM. Lambs were weighed and blood samples obtained by venipuncture weekly. Dietary vitamin E level did not affect performance ( P . 0.05), but lambs fed grass silage grew more slowly ( P , 0.001) and had a higher ( P , 0.001) feed conversion ratio (kg feed/kg gain) than those fed concentrates. At day 0 plasma a-tocopherol concentrations were 0.8 mg/ml and did not differ between treatments ( P . 0.05). Plasma a-tocopherol concentrations then decreased in all lambs except for those fed S-500, which increased, and at slaughter were (mg/ml) 0.07, 0.23, 0.39, 0.76 and 1.57 in C-30, C-60, C-120, C-250 and C-500 and 1.18 and 1.93 in S-60 and S-500, respectively. At slaughter, muscle and liver a-tocopherol concentrations were in the deficiency range for lambs fed C-30, C-60 or C-120, whereas plasma creatine kinase and tissue polyunsaturated fatty acids were unaffected by dietary vitamin E level, but creatine kinase levels were higher ( P , 0.05) and glutathione peroxidise levels lower ( P , 0.001) in lambs fed grass silage than concentrates alone. Muscle and liver a-tocopherol concentrations were 1.8-and 4.1-fold higher in lambs fed S-60 than C-60, but there was less of a difference between lambs fed S-500 or C-500 with muscle and liver differences of 0.4-and 0.7-fold, respectively. Tissue n-3 polyunsaturated fatty acid concentrations were higher ( P , 0.05) and n-6 fatty acids lower in lambs receiving the grass silage compared to concentrate-based diets, but were not affected by dietary vitamin E level. It is concluded that lower plasma and tissue levels of a-tocopherol are present in lambs supplemented with all-rac-a-tocopheryl acetate on a concentrate compared to a mixed diet of silage and concentrates, and that normal growth can be achieved at tissue levels previously considered to represent deficiency.Keywords: a-tocopherol, concentrates, fatty acids, grass silage, lamb growth ImplicationsVitamin E is necessary to protect tissues from oxidative damage. When lambs were fed a concentrate or grass silage-based diet supplemented with increasing amounts of vitamin E, substantially lower plasma and tissue vitamin E levels were observed in lambs receiving the concentratebased diets. Animal performance was, however, unaffected by dietary vitamin E level. Tissue fatty acid composition was also unaffected by...

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Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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“…GSH-Px activity in whole blood was determined using the Olympus AU400 Chemistry Analyzer (Olympus UK, Watford, UK) based on the method of Anderson et al (1978). Results are presented as units per ml.…”

Section: Selenium Analysesmentioning

confidence: 99%

Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in commercial-line turkeys

Juniper

Phipps

Bertin³

2011

Animal

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The objective of this study was to determine the concentration of total selenium (Se) and proportions of total Se comprised as selenomethionine (SeMet) and selenocysteine (SeCys) in the tissues of female turkeys offered diets containing graded additions of selenized-enriched yeast (SY), or sodium selenite (SS). Oxidative stability and tissue glutathione peroxidase (GSH-Px) activity of breast and thigh muscle were assessed at 0 and 10 days post mortem. A total of 216 female turkey poults were enrolled in the study. A total of 24 birds were euthanized at the start of the study and samples of blood, breast, thigh, heart, liver, kidney and gizzard were collected for determination of total Se. Remaining birds were blocked by live weight and randomly allocated to one of four dietary treatments (n 5 48 birds/treatment) that differed either in Se source (SY v. SS) or dose (Con [0.2 mg/kg total Se], SY-L and SS-L [0.3 mg/kg total Se as SY and SS, respectively] and SY-H [0.45 mg total Se/kg]). Following 42 and 84 days of treatment 24 birds per treatment were euthanized and samples of blood, breast, thigh, heart, liver, kidney and gizzard were retained for determination of total Se and the proportion of total Se comprised as SeMet or SeCys. Whole blood GSH-Px activity was determined at each time point. Tissue GSH-Px activity and thiobarbituric acid reactive substances were determined in breast and thigh tissue at the end of the study. There were responses (P , 0.001) in all tissues to the graded addition of dietary Se, although rates of accumulation were highest in birds offered SY. There were notable differences between tissue types and treatments in the distribution of SeMet and SeCys, and the activity of tissue and erythrocyte GSH-Px (P , 0.05). SeCys was the predominant form of Se in visceral tissue and SeMet the predominant form in breast tissue. SeCys contents were greater in thigh when compared with breast tissue. Muscle tissue GSH-Px activities mirrored SeCys contents. Despite treatment differences in tissue GSH-Px activity, there were no effects of treatment on any meat quality parameter.

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“…In the Czech Republic a correlation between concentration of selenium and activity of glutathione peroxidase in whole blood has not been described for sheep. There exist articles from other countries on sheep, in which some authors describe a positive correlation (Anderson et al 1978;Verde et al 1995), whilst others have not found any correlation (Rosen et al 2009). …”

mentioning

confidence: 98%

Direct and indirect assessment of selenium status in sheep - a comparison

Pavlata¹,

Mišurová²,

Pechová³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the relationship between selenium concentration and activity of glutathione peroxidase (GSH-Px) in the whole blood of sheep and reference ranges for the activity of GSH-Px for evaluation of the selenium status of sheep in the Czech Republic. Selenium concentration and activity of glutathione peroxidase were determined in whole blood samples collected from 92 sheep in six herds. The GSH-Px activity in the samples was measured using the photometric method. Selenium in the sample was measured using the hydride technique atomic absorption spectrometry. Data on both parameters were processed using correlation and regression analysis in order to obtain reference values of GSH-Px for the indirect evaluation of the selenium status of sheep. The two variables showed a close and significant correlation (r = 0.95; P < 0.01). The regression line, defined by the equation y = 7.5857x -121.87 (linear) or y = -0.0167x 2 + 11.993x -355.57 (polynomial), allowed us to determine the GSH-Px activity of 637, resp. 677 μkat/l as equivalent to selenium concentration in whole blood of sheep 100 μg/l. Mean selenium concentration and mean GSH-Px activity found in whole blood samples were 123.42 ± 57.84 μg/l and 814.34 ± 463.64 μkat/l, respectively. In this study, a close dependence of GSH-Px activity on the selenium concentration in the blood of sheep was found. Activity values of GSH-Px were determined for use in the diagnosis of sufficient selenium status in sheep in the Czech Republic (GSH-Px activity greater than 600 μkat/l of whole blood).

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Glutathione peroxidase activity in erythrocytes and muscle of cattle and sheep and its relationship to selenium

Cited by 68 publications

References 19 publications

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in commercial-line turkeys

Direct and indirect assessment of selenium status in sheep - a comparison

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