Glutathione Metabolism Under High-Altitude Stress and Effect of Antioxidant Supplementation

Vats, Praveen; Singh, Vijay Kumar; Singh, Som Nath; Singh, Shashi Bala

doi:10.3357/asem.2305.2008

Cited by 34 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In previous publications, it was shown that the exposure of male rats to continuous chronic hypobaric hypoxia and intermittent chronic hypobaric hypoxia induced evident changes in testicular morphology, loss of germinal cells, arrest of spermatogenesis and metabolic stress in the mitochondria of round spermatids, consistent with oxygen consumption processes related to lipid peroxidation (Farias et al, 2005a). Furthermore, high altitude exposure has been shown to induce oxidative stress (Vats et al, 2008) that is accompanied by decreased levels of reduced glutathione and ascorbic acid and by an increase in antioxidant enzymes like glutathione reductase. Thus, a likely mechanism of hypobaric hypoxia-induced inhibition of spermatogenesis and sperm production can be related to an oxidative stress inmale reproductive organs (Farias et al, 2005a).…”

Section: Introductionmentioning

confidence: 94%

Effect of High Altitude Hypoxia on the Postnatal Development of Rat Testis: Histological and Histometric Study

Mohammed

Tingari

2016

Int. J. Morphol.

View full text Add to dashboard Cite

Effect of high altitude hypoxia on the postnatal development of rat testis: Histological and histometric study. Int. J. Morphol., 34(2):610-615, 2016. SUMMARY:The study was carried out at two different altitudes in the southern region of Saudi Arabia: Abha, 2,800 meters above sea level, the high altitude (HA) area and Jazan, 40 meters above sea level the low altitude (LA) area. Following exposure to high altitude, and up to the third week of postnatal development, some of the seminiferous tubules showed detachment from the basal lamina, spaces of different sizes within the epithelial layer with vacuoles in the center and Pyknotic nuclei were noted in the spermatogonia and primordial germ cells. Rounded spermatids were seen in the lumen only on day 35 of hypoxic group, no spermatozoa were recognized until day 45 of postnatal development. On day 45 hypoxic rat testes revealed various types of atrophy and degeneration in the seminiferous tubules and in the interstitial tissue, there was detachment of the basal laminae of the tubules and a profound decrease in cellularity. Significant decrease in epithelial height was noticed in these animals (P <0.05). Also, the diameter of the tubules showed slight decrease with concomitant increase in interstitial spaces in all hypoxic rats.

show abstract

Section: Introductionmentioning

confidence: 94%

Effect of High Altitude Hypoxia on the Postnatal Development of Rat Testis: Histological and Histometric Study

Mohammed

Tingari

2016

Int. J. Morphol.

View full text Add to dashboard Cite

show abstract

“…As oxygen is the critical constituent of aerobic life, the main problem experienced by humans at HA is diminished O 2 availability leading to altered metabolism and subsequent oxidative stress (Magalhaes et al 2004). Several studies have reported the evidence of oxidative stress at HA both in humans and animals (Joanny et al 2001;Magalhaes et al 2004Magalhaes et al , 2005Nakanishi et al 1995;Dosek et al 2007;Bakonyi and Radak 2004;Chao et al 1999;Jefferson et al 2004;Vij et al 2005;Vats et al 2008). Most of these studies have considered hypoxia and extreme cold as an inseparable factor at HA and therefore evaluated the overall effect of HA exposure on oxidative stress.…”

Section: Introductionmentioning

confidence: 97%

Antioxidant and oxidative stress responses of sojourners at high altitude in different climatic temperatures

et al. 2009

View full text Add to dashboard Cite

High altitude (HA) is a multi-stressor environment comprising hypobaric hypoxia and cold. Climatic temperature varies with seasonal variation at HA. The present study was undertaken to investigate the effect of ambient temperature on antioxidant profile among sojourners at HA. The study was conducted on sojourners exposed to an altitude of 4,560 m in two different seasons and categorized into two groups (SOJ 1, n=63, ambient temp. at HA: -6 degree to +10degreeC; SOJ 2, n=81, ambient temp. at HA: 3degree-22degreeC). Blood was collected at sea level (SL) and after 4 weeks of HA exposure. Antioxidant enzymes showed significant upregulation in SOJ 2 at HA. In SOJ 1, superoxide dismutase and glutathione peroxidase showed significant upregulation but catalase and glutathione reductase showed significant decrease at HA. Non-enzymatic antioxidants showed significant reduction in SOJ 1 whereas a sustained antioxidant profile was observed in SOJ 2 at HA. Oxidative stress markers showed higher levels in SOJ 1 than SOJ 2 at HA. Differences observed between SOJ 1 and SOJ 2 at HA may be the consequence of different environmental temperatures. Cold stress was higher in SOJ 1 as evidenced from the significantly lower oral temperature in SOJ 1 as compared to SOJ 2. Cold- and hypoxia-induced increase in energy expenditure was significantly high in SOJ 1 than SOJ 2. To conclude, chronic exposure to hypoxia in moderate climatic temperature has a potential preconditioning effect on antioxidant system, but exposure to both cold and hypoxia causes greater oxidative stress due to altered metabolic rate.

show abstract

“…Reduced glutathione (GSH) and oxidized glutathione (GSSG) in the liver tissue were estimated using the method proposed by Vats et al (36). Fifty ml of the homogenates of liver tissue was treated with 450 ml solution of 10% m-phosphoric acid and centrifuged for 10 min at 1000 x g. For GSH determination, 0.1 ml supernatant was used diluted with 1.8 ml of 0.1M phosphate buffer (pH 8) containing 5 nmol/L EDTA, to which 0.1 ml of o-phthalaldehyde in methanol (1 mg/ml) was added.…”

Section: Measurement Of Biochemical Markers Of Oxidative Stressmentioning

confidence: 99%

Protective effects of Quercetin and chronic moderate exercise (training) against oxidative stress in the liver tissue of streptozotocin-induced diabetic rats

Chiş

Muresan

Oros

et al. 2016

Acta Physiologica Hungarica

View full text Add to dashboard Cite

Background: To investigate the protective effects of Quercetin administration associated with chronic moderate exercise (training) on oxidative stress in the liver in streptozotocin-induced diabetic rats. Methods: Diabetic rats that performed exercise training were subjected to a swimming training program (1 hour/day, 5 days/week, 4 weeks). The diabetic rats received natural antioxidant, Quercetin (20 mg/kg body weight/day) for 4 weeks. At the end of the study, all animals were sacrificed and liver samples were collected for estimation: some oxidative stress markers (malondialdehyde, MDA and protein carbonyls groups, PC), the activity of antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT), reduced glutathione (GSH) level and reduced (GSH) and oxidized (GSSG) glutathione ratio. Results: Diabetic rats submitted to exercise training showed significantly increased the oxidative stress markers (MDA and PC) and a reduction of antioxidant enzyme (SOD and CAT) activity, GSH level and GSH/ GSSG ratio in hepatic tissues. A decrease in the levels of oxidative stress markers associated with elevated activity of antioxidant enzymes, the GSH level and GSH/GSSG ratio in the hepatic tissue were observed in Quercetin-treated diabetic trained rats. Conclusions: These findings suggest that Quercetin administration in association with chronic moderate exercise exerts a protective effect in diabetes by attenuating hyperglycemia-mediated oxidative stress in hepatic tissue.

show abstract

Glutathione Metabolism Under High-Altitude Stress and Effect of Antioxidant Supplementation

Abstract: Results indicate that HA exposure adversely affects glutathione metabolism and antioxidant defense mechanisms and these changes can be ameliorated through supplementation of NAC and vitamin E.

Cited by 34 publications

References 0 publications

Effect of High Altitude Hypoxia on the Postnatal Development of Rat Testis: Histological and Histometric Study

Effect of High Altitude Hypoxia on the Postnatal Development of Rat Testis: Histological and Histometric Study

Antioxidant and oxidative stress responses of sojourners at high altitude in different climatic temperatures

Protective effects of Quercetin and chronic moderate exercise (training) against oxidative stress in the liver tissue of streptozotocin-induced diabetic rats

Contact Info

Product

Resources

About