Glutathione in combination with trehalose has supplementary beneficial effects on cryopreserved red deer (<i>cervus elaphus</i>) sperm

Wang, Yan; Dong, Shu-De

doi:10.1111/aji.12610

Cited by 8 publications

(13 citation statements)

References 24 publications

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“…Ansari et al (2014) observed that the addition of 0.5mM glutathione in semen extender improved the motility, plasma membrane integrity, viability and acrosomal integrity of Sahiwal bull spermatozoa. These results are in agreement with Wang and Dong (2017) who reported that glutathione addition to the extender improved sperm quality, including sperm motility, acrosomal integrity, plasma-membrane integrity and sperm morphology. Similarly, Gadea et al (2005) reported beneficial effect of GSH in cryopreserved boar spermatozoa in terms of acrosomal intactness and percentage of non-capacitated spermatozoa, corroborated the findings of this study.…”

Section: Resultssupporting

confidence: 93%

“…Glutahione was reported to be used as a cryoprotectant for the sperm of mouse, canine, boar, ovine, bull and red deer (Sławeta & Laskowska, 1987, Takeo & Nakagata 2011, Perez et al, 2012, Anel-López et al, 2015and Lucio et al, 2016. Recently, Wang and Dong (2017) reported that glutathione addition to the extender improved sperm quality, including sperm motility, acrosomal integrity, plasma-membrane integrity and sperm morphology.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Shamiah¹,

El-Karim

Eshera³

2017

Journal of Sustainable Agricultural Sciences

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IntroductionThe increasing use of artificial insemination (AI) in poultry industry emphasizes the need for good quality sperm distribution. A proper evaluation of semen prior to AI or storage is very important to avoid losses in fertility. Because semen evaluation is extremely important for semen storage and AI programs, a rapid, economical, objective and fertility predictive method of semen evaluation would be beneficial for the poultry industry protocols. Determination of antioxidant enzymes concentration in semen has been practiced for a long time (Peters, 2008).Lipids are a basic component of semen, contributing to the membrane structure of spermatozoa, the metabolism of the sperm cells, and their ability to capacitate and fertilize the female gamete. In birds, the lipid composition of spermatozoa has an influence on fertility (Ansah and Buckland, 1982). El-Saadany (2002) reported that increasing storage time of diluted semen from local chicken breed for 0, 24, 48 and 72 hr decreased significantly (P<0.01) the fertility rate (FR) by 5.12, 9.55 and 12.53%, respectively. Shamiah et al. (2017) reported that GSH supplementation in semen extender significantly (P<0.05) increased FR. Semen supplemented with 0.2 mM GSH (74.5%) showed higher FR than those supplemented with 0.6 mM GSH or free semen (59.9 and 59.2%, respectively).Sperm cells have a high content of unsaturated T HIS STUDY was carried out to evaluate the effect of glutathione (GSH) supplementation at levels of 0.0 (control), 0.2, 0.4 and 0.6 mM to diluted semen stored for 0, 24, 48 and 72 hr at 5°C, on antioxidant enzymes activities in seminal plasma, semen characteristics and fertility rate (FR) of cocks Inshas strain. Supplementation of 0.6 mM GSH to the diluted cocks semen significantly increased (P<0.05) the concentration of alkaline phosphatase (ALP) (40.2 u/ml) compared to other treatments (33.0, 27.8 and 28.3 u/ml). Superoxide dismutase (SOD) concentration in seminal plasma was not significantly affected (P<0.05) by different levels of GSH, storage period or interaction between levels of GSH and storage period. Glutathione peroxidase (GSH-Px) concentration in seminal plasma was not affected by different levels of GSH (P<0.05). Total antioxidant capacity (TACO) in seminal plasma was significantly affected (P<0.05) by different levels of GSH, storage period or interaction between them. There was a positive correlation (r=0.089 and r = 0.168) between SOD concentration and (GSH-Px and TAOC) concentration, but negative correlation (r=-0.119) with ALP concentration. Positive insignificant correlation between (ALP, SOD and TACO) concentration in seminal plasma with the progressive motility, live sperm, curled tail and fertility, while positive significant correlation between GSH-Px concentration with progressive motility, curled tail and fertility. It could be concluded that the addition of GSH to semen extender of cockerels especially at levels 0.2 mM improved the antioxidant enzymes activities, which play a vital role in lowering the deleterious effe...

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Shamiah¹,

El-Karim

Eshera³

2017

Journal of Sustainable Agricultural Sciences

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“…The vast majority of available studies show coherent results, even though there are contradictory ones demonstrating higher concentrations of GSH in patients with azoospermia compared to oligozoospermia or normozoospermia [ 49 ]. Several studies demonstrated that the addition of GSH to cryopreserved ejaculate of animals protects the sperm from the harmful effects of ROS accumulated during this process, at the same time improving sperm motility, viability, the integrity of the cell membrane, and sperm DNA, as well as the activity of antioxidant enzymes [ 50 , 51 , 52 ]. The addition of cysteine to rabbit ejaculate increased biosynthesis of intracellular glutathione and significantly affected cell membrane integrity, acrosomal inviolability or sperm motility [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Sperm Antioxidant Biomarkers and Their Correlation with Clinical Condition and Lifestyle with Regard to Male Reproductive Potential

Krzyściak

Papież

Bąk³

et al. 2020

JCM

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Measurement of sperm oxidative-antioxidant indicators is widely used in the assessment and detection of biochemical causes of male infertility. The main purpose of this study was to identify biomarkers that assist in diagnostics and monitoring of male reproductive potential. We performed the assessment of oxidative-antioxidant malondialdehyde (MDA), glutathione (GSH), and total redox antioxidant potential (TRAP) indicators in seminal plasma, seminogram, clinical condition, and lifestyle of people with reproductive problems. The combined assessment of GSH and TRAP as potential biomarkers of male infertility in semen plasma was characterized by the highest total sensitivity and specificity. Furthermore, we provide evidence that male reproductive potential is significantly correlated with basic sperm parameters, sperm cell membrane integrity, their morphology, lifestyle, eating habits, occupation, and mental health. Our results provide evidence on the importance of oxidative stress and defense against free radicals in diagnosing and monitoring men with infertility that are consistent with previously conducted research. We provide an alternative approach on the possibility of interpreting the combination of the biomarkers that can bring benefits to a multi-threaded approach to the diagnosis and treatment of male infertility.

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“…Trehalose (Tre) is a stabilizer of the lipid membrane, protein, and biofilm system. During the cryopreservation of sperm cells, it acts as a protective agent to reduce the formation of intracellular ice crystals, to maintain protein structure, and significantly improve the mobility of sperm cells, as well as acrosome and cell membrane stability, reducing sperm cell aberrations or lesions (Wang and Dong, 2017). The addition of antioxidants to the cryoprotectant contributes to the protection of sperm cells from the adverse effects of reactive oxygen species and improves sperm cell recovery survival.…”

Section: Introductionmentioning

confidence: 99%

Trehalose addition to a Tris-fructose egg yolk extender on quality of ram sperm preserved at 0 °C

Zhao¹,

Xiao²,

Zhu³

et al. 2020

Revista Brasileira De Zootecnia

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The present study investigated the effects of various concentrations of trehalose in Tris-fructose egg yolk diluent on ram semen preservation at 0 ℃. Semen was collected by artificial vagina ejaculation from six rams of proven fertility. Highquality ejaculates were diluted with 0 (control), 5, 10, 15, and 20 mM trehalose of Tris-fructose egg yolk extender and control (tris-fructose egg yolk extender without trehalose), respectively. Then, the ejaculates were diluted to a concentration of 5×10 8 sperm/mL, cooled to 0 ℃ for 90 min, and maintained at that temperature for twelve days. The diluted semen samples were examined, and their sperm progressive motility, membrane functionality, and acrosome integrity recorded at 0, 24, 72, 144, 216, and 288 h. Two hundred ninety-six ewes were transcervically inseminated with the 216-h control (without trehalose) or the optimal trehalose concentration group semen, and the pregnancy and lambing rates were measured. No significant differences were established in the sperm progressive motility and membrane functionality among the control and 5, 10, 15, and 20 mM groups. The sperm samples of trehalose addition groups had no significant difference in the acrosome integrity of sperm, but they were, nonetheless, significantly higher than those in the control. No significant difference was detected in the lambing and pregnancy rates between the 5 mM and control groups. These results suggest that ram sperm is capable of fertilization after cooling and preservation at 0 ℃ by the use of 5 mM trehalose for Tris-fructose egg yolk diluent. Under these conditions, ram sperm can be more effectively preserved than under other four concentrations of diluents.

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Glutathione in combination with trehalose has supplementary beneficial effects on cryopreserved red deer (cervus elaphus) sperm

Abstract: Therefore, glutathione in combination with trehalose could be a promising cryoprotectant for red deer sperm.

Cited by 8 publications

References 24 publications

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Sperm Antioxidant Biomarkers and Their Correlation with Clinical Condition and Lifestyle with Regard to Male Reproductive Potential

Trehalose addition to a Tris-fructose egg yolk extender on quality of ram sperm preserved at 0 °C

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