Glutathione half-cell reduction potential: A universal stress marker and modulator of programmed cell death?

Kranner, Ilse; Birtić, Simona; Anderson, Kim; Pritchard, Hugh W.

doi:10.1016/j.freeradbiomed.2006.02.013

Cited by 289 publications

(350 citation statements)

References 61 publications

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“…Change in the GSH-and GSSG-dependent redox potential was suggested to be a marker of the stress-induced damages (Kranner et al, 2006). NaCl had a great effect on the redox environment in the maize leaves as it was shown by the increase in the amount of GSSG and in the redox potential of the GSH/GSSG redox pair.…”

Section: Gene Expression Studiesmentioning

confidence: 99%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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Abstract:It was assumed that salt-induced redox changes affect amino acid metabolism in maize (Zea mays L.), and this influence may be modified by NO. The applied NaCl treatment reduced the fresh weight of shoots and roots. This decrease was smaller after the combined application of NaCl and an NO-donorETA/NO) in the shoots, while it was greater after simultaneous treatment with NaCl and nitro-l-arginine (l-NNA, inhibitor of NO synthesis) in the roots. The quantum yield efficiency of photosystem II was not influenced by the treatments. NaCl had a significant effect on the redox environment in the leaves as it was shown by the increase in the amount of glutathione disulphide and in the redox potential of the glutathione/glutathione disulphide redox pair. This influence of NaCl was modified by DETA/NO and l-NNA. Pharmacological modification of NO levels affected salt-induced changes in both the total free amino acid content and in the free amino acid composition. NaCl alone increased the concentration of almost all amino acids which effect was strengthened by DETA/NO in the case of Pro. l-NNA treatment resulted in a significant increase in the Ala, Val, Gly and Tyr contents. The Ile, Lys and Val concentrations rose considerably after the combined application of NaCl and DETA/NO compared to NaCl treatment alone in the recovery phase. NaCl also increased the expression of several genes related to the amino acid and antioxidant metabolism, and this effect was modified by DETA/NO. In conclusion, modification of NO levels affected salt-induced, glutathione-dependent redox changes and simultaneously the free amino acid composition and the level of several free amino acids. The observed much higher Pro content in plants treated with both NaCl and DETA/NO during recovery may contribute to the protective effect of NO against salt stress.

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Section: Gene Expression Studiesmentioning

confidence: 99%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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“…1B). An observed shift of GSH/GSSG, to a more oxidized state with a seedling age, resulted in more positive values for half-cell reduction potential of the GSH/GSSG couple (E GSSG/2GSH ) by approximately 47 mV (calculated on the basis of GSH and GSSG concentration, Nernst equation) (Meyer and Hell 2005;Kranner et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…It is also present in the cytoplasm, mitochondria and peroxisomes (Noctor and Foyer 1998). Changes in the glutathione redox state, defined by the reducing capacity of GSH (GSH concentration) and the half-cell reduction potential of the GSH/GSSG couple (E GSSG/2GSH ) has been proposed as a marker of the cellular redox state (Kranner et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings

2015

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The present work demonstrates that the transition of wheat (Triticum aestivum L.) seedlings from dehydration tolerance to intolerance on the fifth day following imbibition is associated with a disturbance in cellular redox homeostasis. During germination the ratio of reduced (AsA) to oxidized ascorbate (DHA) was lower in the tolerant 4-day-old seedlings compared to the sensitive 6-day-old seedlings because of the lower ascorbate content in the former. The reduced glutathione (GSH) and total glutathione (GSH ? GSSG) pools were higher in tolerant seedlings and remained higher upon dehydration. The development of dehydration intolerance with a seedling age coincided with a 50 % loss of the total glutathione pool and a shift of GSH/GSSG to a more oxidized state. Activities of ascorbate peroxidase and glutathione reductase increased with water deficiency in both tolerant and sensitive seedlings but the three new activity bands appeared only in sensitive seedlings. The stable ratio of GSH/GSSG and a higher AsA/DHA ratio in sensitive seedlings did not prevent the enhanced production of H2O2 and the peroxidation of lipids with dehydration. As a result, an increase in the protein carbonyl group and a significant decrease in the thiol groups were observed in dehydrated sensitive seedlings. Water deficiency enhanced the total azocaseinolytic activity, mainly in sensitive seedlings. The highest increase in protein carbonylation and lowest azocaseinolytic activity was observed at the beginning of seedling dehydration (15-25 % WSD) being in a sensitive phase of growth. The presented results indicate that the development of dehydration intolerance during the transition of wheat seedlings from heterotrophic to autotrophic growth is associated with an alterations in protein oxidation.

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“…Glutathione (GSH) is one of the main water soluble sacrificial antioxidants in plants (Kranner et al, 2006). This low molecular weight thiol is converted to its oxidised form (GSSG) upon interaction with ROS.…”

Section: Antioxidantsmentioning

confidence: 99%

“…This low molecular weight thiol is converted to its oxidised form (GSSG) upon interaction with ROS. The ratio of reduced to oxidised GSH is a good indicator of oxidative stress, as an increased amount of GSSG indicates the inability of the plant to control oxidative damage, thereby triggering premature cell death (Kranner et al, 2006). GSH is recycled by the enzyme GSH reductase using NADPH as the electron donor (Halliwell & Gutteridge, 2007).…”

Section: Antioxidantsmentioning

confidence: 99%