Glutathione, glutathione-related enzymes, and oxidative stress in individuals with subacute occupational exposure to lead

Dobrakowski, Michał; Pawlas, Natalia; Hudziec, Edyta; Kozłowska, Agnieszka; Mikołajczyk, Agnieszka; Birkner, Ewa; Kasperczyk, Sławomir

doi:10.1016/j.etap.2016.06.008

Cited by 29 publications

(15 citation statements)

References 22 publications

(23 reference statements)

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“…The levels of serum MDA and LPS did not change significantly. Consistently, in our previous study [ 8 ], we showed increased total oxidant status (TOS) level in leukocytes, unchanged leukocyte and erythrocyte MDA level, and decreased erythrocyte glutathione pool due to a short-term lead exposure. Therefore, it seems that the primary products of lipid peroxidation, such as LPH or TOS value, are better in estimating oxidative stress intensity in acute or subacute lead exposure.…”

Section: Discussionsupporting

confidence: 91%

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The Effect of a Short‐Term Exposure to Lead on the Levels of Essential Metal Ions, Selected Proteins Related to Them, and Oxidative Stress Parameters in Humans

Dobrakowski

Boroń

Birkner

et al. 2017

Oxidative Medicine and Cellular Longevity

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The present study was designed to explore the possible influence of subacute exposure to lead on the levels of selected essential metals, selected proteins related to them, and oxidative stress parameters in occupationally exposed workers. The study population included 36 males occupationally exposed to lead for 36 to 44 days. Their blood lead level at the beginning of the study was 10.7 ± 7.67 μg/dl and increased to the level of 49.1 ± 14.1 μg/dl at the end of the study. The levels of calcium, magnesium, and zinc increased significantly after lead exposure compared to baseline by 3%, 3%, and 8%, respectively, while the level of copper decreased significantly by 7%. The malondialdehyde (MDA) level and the activities of catalase (CAT) and superoxide dismutase (SOD) did not change due to lead exposure. However, the level of lipid hydroperoxides (LPH) in serum increased significantly by 46%, while the level of erythrocyte lipofuscin (LPS) decreased by 13%. The serum levels of essential metals are modified by a short-term exposure to lead in occupationally exposed workers. A short-term exposure to lead induces oxidative stress associated with elevated levels of LPH but not MDA.

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Section: Discussionsupporting

confidence: 91%

“…We followed the methods of Dobrakowski et al [ 8 ]. To obtain whole blood, erythrocytes, and leukocytes, 14 ml of blood was drawn by venipuncture into tubes containing an EDTA solution as an anticoagulant.…”

Section: Methodsmentioning

confidence: 99%

The Effect of a Short‐Term Exposure to Lead on the Levels of Essential Metal Ions, Selected Proteins Related to Them, and Oxidative Stress Parameters in Humans

Dobrakowski

Boroń

Birkner

et al. 2017

Oxidative Medicine and Cellular Longevity

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View full text Add to dashboard Cite

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“…AA; Varian or AAS). The quantity of Pb was given in mg/g of feces (wet weight) and μg/g of tissue ( Dobrakowski et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

“…The MDH, GSH level, activities of GSH-Px, SOD, CAT in the mice tissues while, AST and ALT in the serum were determined with the respective kits obtained from the Jiancheng Bioengineering Institute. All assays were done in triplicate in accordant to the manufacturer’s guidelines ( Dobrakowski et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Comparative Assessment of the Bioremedial Potentials of Potato Resistant Starch-Based Microencapsulated and Non-encapsulated Lactobacillus plantarum to Alleviate the Effects of Chronic Lead Toxicity

et al. 2018

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Lead (Pb) is a well-recognized and potent heavy metal with non-biodegradable nature and can induce the oxidative stress, degenerative damages in tissues, and neural disorders. Certain lactic acid bacterial strains retain the potential to mitigate the lethal effects of Pb. The present work was carried out to assess the Pb bio-sorption and tolerance capabilities of Lactobacillus plantarum spp. Furthermore, potato resistant starch (PRS)-based microencapsulated and non-encapsulated L. plantarum KLDS 1.0344 was utilized for bioremediation against induced chronic Pb toxicity in mice. The experimental mice were divided into two main groups (Pb exposed and non-Pb exposed) and, each group was subsequently divided into three sub groups. The Pb exposed group was exposed to 100 mg/L Pb(NO3)2 via drinking water, and non-Pb exposed group was supplied with plain drinking water during 7 weeks prolonged in vivo study. The accumulation of Pb in blood, feces, renal, and hepatic tissues and its pathological damages were analyzed. The effect of Pb toxicity on the antioxidant enzyme capabilities in blood, serum, as well as, on levels of essential elements in tissues was also calculated. Moreover, KLDS 1.0344 displayed remarkable Pb binding capacity 72.34% and Pb tolerance (680 mg/L). Oral administration of both non- and PRS- encapsulated KLDS 1.0344 significantly provided protection against induced chronic Pb toxicity by increasing fecal Pb levels (445.65 ± 22.28 μg/g) and decreasing Pb in the blood up to 137.63 ± 2.43 μg/L, respectively. KLDS 1.0344 microencapsulated with PRS also relieved the renal and hepatic pathological damages and improved the antioxidant index by inhibiting changes in concentrations of glutathione peroxidase, glutathione, superoxide dismutase, malondialdehyde, and activated oxygen species, which were affected by the Pb exposure. Overall, our results suggested that L. plantarum KLDS 1.0344 either in free or encapsulated forms hold the potentiality to deliver a dietetic stratagem against Pb lethality.

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“…O Pb e outros metais, incluindo cádmio e mercúrio, estimulam a expressão de GST em diferentes tecidos, como fígado, rins e cérebro (WRIGHT et al, 1998;BRAMBILA et al, 2002;CASALINO et al, 2006). A correlação entre Pb-s e o aumento GST já foi descrita em outros estudos envolvendo exposição ocupacional ao Pb (CONTERATO et al, 2013;DOBRAKOWSKI et al, 2016) Um dos alvos das EROs são os ácidos nucleicos e, por este motivo, os ensaios que avaliam a oxidação de DNA através de 8-OHdG urinária têm sido empregados como ferramentas úteis em estudos de exposição a xenobióticos (CHANG et al, 2011;PRASAD et al, 2013;LI et al, 2014). O interesse crescente na dosagem deste biomarcador se deve à conhecida relação entre a oxidação de DNA e os processos de envelhecimento (SIOMEK et al, 2007;RADAK;BOLDOGH, 2010) e carcinogênese (PYLVÄS et al, 2011;TABUR et al, 2015).…”

Section: O Estresse Oxidativo é Um Dos Efeitos Tóxicos Mais Relevanteunclassified

Exposição ocupacional ao chumbo (Pb): efeitos sobre o estado redox celular e a influência de polimorfismos genéticos sobre a toxicocinética do metal

Gomes¹

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Glutathione, glutathione-related enzymes, and oxidative stress in individuals with subacute occupational exposure to lead

Cited by 29 publications

References 22 publications

The Effect of a Short‐Term Exposure to Lead on the Levels of Essential Metal Ions, Selected Proteins Related to Them, and Oxidative Stress Parameters in Humans

The Effect of a Short‐Term Exposure to Lead on the Levels of Essential Metal Ions, Selected Proteins Related to Them, and Oxidative Stress Parameters in Humans

Comparative Assessment of the Bioremedial Potentials of Potato Resistant Starch-Based Microencapsulated and Non-encapsulated Lactobacillus plantarum to Alleviate the Effects of Chronic Lead Toxicity

Exposição ocupacional ao chumbo (Pb): efeitos sobre o estado redox celular e a influência de polimorfismos genéticos sobre a toxicocinética do metal

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