Glutathione and Related Enzymes in Response to Abiotic Stress

Štolfa, Ivna; Maronić, Dubravka Špoljarić; Pfeiffer, Tanja Žuna; Lončarić, Zdenko

doi:10.1007/978-3-319-44081-1_9

Cited by 7 publications

(3 citation statements)

References 199 publications

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“…High salinity is a severe limiting factor among all the abiotic stresses, which can negatively affect the crop production (Maskri et al, 2010;Yasar et al, 2016). Abiotic stresses can lead to an enhanced production of reactive oxygen species (ROS) in plants, due to the disruption of cellular homeostasis, causing damage to proteins, DNA, lipids, activation of the programmed cell death (PCD) pathway and ultimately leading to different tissues death (Cuypers et al, 2010;Stolfa et al, 2016;Yasar et al, 2016). Once formed, the cascade of uncontrolled oxidation reactions by these ROS can be disrupted by enzymatic and non-enzymatic mechanisms to maintain the adequate cell redox state (Gratão et al, 2015;Alves et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The partial root-zone saline irrigation system and antioxidant responses in tomato plants

Alves

Medeiros

Nicolau

et al. 2018

Plant Physiology and Biochemistry

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Salinity is a limiting factor that can affect plant growth and cause significant losses in agricultural productivity. This study provides an insight about the viability of partial root-zone irrigation (PRI) system with saline water supported by a biochemical approach involving antioxidant responses. Six different irrigation methods using low and high salt concentrations (S1-0.5 and S2-5.0 dS m) were applied, with or without PRSI, so that one side of the root-zone was submitted to saline water while the other side was low salinity water irrigated. The results revealed different responses according to the treatments and the PRSI system applied. For the treatments T1, T2 and T3, the PRSI was not applied, while T4, T5 and T6 treatments were applied with PRSI system. Lipid peroxidation, proline content, and activities of SOD, CAT, APX, GR and GSH in tomato plants subjected to PRSI system were analyzed. Plant growth was not affected by the salt concentrations; however, plants submitted to high salt concentrations showed high MDA content and Na accumulation when compared to the control plants. Plants submitted to treatments T4, T5 and T6 with PRSI system exhibited lower MDA compared to the control plants (T1). Proline content and activities of SOD, CAT, APX, GR and GSH content were maintained in all treatments and tissues analyzed, with only exception for APX in fruits and GSH content, in roots. The overall results showed that PRSI system could be an applicable technique for saline water supply on irrigation since plants did not show to be vulnerable to salt stress, supported by a biochemical approach involving antioxidant responses.

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Section: Introductionmentioning

confidence: 99%

The partial root-zone saline irrigation system and antioxidant responses in tomato plants

Alves

Medeiros

Nicolau

et al. 2018

Plant Physiology and Biochemistry

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“…The results of studies conducted in different transgenic and wild-type plants, Zn accumulators, hyperaccumulators, and Zn-resistant plant genotypes, showed that higher levels of GSH and increased activity of enzymes involved in the metabolism of GSH result in intrinsic resistance to excess HMs [ 36 , 37 , 38 ]. GSH is a critical component of the antioxidant system and is involved in a wide range of physiological processes in the cell [ 39 , 40 ]. In addition to the antioxidant role, the nucleophilic nature of the thiol groups is particularly important in the detoxification of the redox-inert electrophiles such as Zn due to the direct conjugation of GSH with metal ions.…”

Section: Discussionmentioning

confidence: 99%

Wheat Leaf Antioxidative Status—Variety-Specific Mechanisms of Zinc Tolerance during Biofortification

Čamagajevac

Vuković

et al. 2021

Plants

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In this study, we evaluated the leaf antioxidative responses of three wheat varieties (Srpanjka, Divana, and Simonida) treated with two different forms of zinc (Zn), Zn-sulfate and Zn-EDTA, in concentrations commonly used in agronomic biofortification. Zn concentration was significantly higher in the flag leaves of all three wheat varieties treated with Zn-EDTA compared to control and leaves treated with Zn-sulfate. Both forms of Zn increased malondialdehyde level and total phenolics content in varieties Srpanjka and Divana. Total glutathione content was not affected after the Zn treatment. Zn-sulfate increased the activities of glutathione reductase (GR) and guaiacol peroxidase (GPOD) in both Srpanjka and Divana, while glutathione S-transferase (GST) was only induced in var. Srpanjka. Chelate form of Zn increased the activities of GST and GPOD in both Simonida and Divana. Catalase activity was shown to be less sensitive to Zn treatment and was only induced in var. Srpanjka treated with Zn-EDTA where GPOD activity was not induced. Concentrations of Zn used for agronomic biofortification can induce oxidative stress in wheat leaves. The antioxidative status of wheat leaves could be a good indicator of Zn tolerance, whereas wheat genotype and chemical form of Zn are the most critical factors influencing Zn toxicity.

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“…Various essential antioxidant mechanisms have evolved to regulate ROS levels (especially H 2 O 2 ), such as the ascorbate–glutathione (AsA–GSH) pathway and its related enzymes [ 29 ]. The total amounts of non-enzymatic antioxidants (e.g., GSH, AsA, and flavonoids) and their redox status (ascorbate/dehydroascorbate: AsA/DHA; and reduced/oxidized forms of glutathione: GSH/GSSG) play crucial roles in cellular processes [ 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants

Horváth,

Kulman,

Tompa

et al. 2023

Antioxidants

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Glutathione transferases (GSTs) are one of the most versatile multigenic enzyme superfamilies. In our experiments, the involvement of the genotype-specific induction of GST genes and glutathione- or redox-related genes in pathways regulating salt-stress tolerance was examined in tomato cultivars (Solanum lycopersicum Moneymaker, Mobil, and Elán F1). The growth of the Mobil plants was adversely affected during salt stress (100 mM of NaCl), which might be the result of lowered glutathione and ascorbate levels, a more positive glutathione redox potential (EGSH), and reduced glutathione reductase (GR) and GST activities. In contrast, the Moneymaker and Elán F1 cultivars were able to restore their growth and exhibited higher GR and inducible GST activities, as well as elevated, non-enzymatic antioxidant levels, indicating their enhanced salt tolerance. Furthermore, the expression patterns of GR, selected GST, and transcription factor genes differed significantly among the three cultivars, highlighting the distinct regulatory mechanisms of the tomato genotypes during salt stress. The correlations between EGSH and gene expression data revealed several robust, cultivar-specific associations, underscoring the complexity of the stress response mechanism in tomatoes. Our results support the cultivar-specific roles of distinct GST genes during the salt-stress response, which, along with WRKY3, WRKY72, DREB1, and DREB2, are important players in shaping the redox status and the development of a more efficient stress tolerance in tomatoes.

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Glutathione and Related Enzymes in Response to Abiotic Stress

Cited by 7 publications

References 199 publications

The partial root-zone saline irrigation system and antioxidant responses in tomato plants

The partial root-zone saline irrigation system and antioxidant responses in tomato plants

Wheat Leaf Antioxidative Status—Variety-Specific Mechanisms of Zinc Tolerance during Biofortification

Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants

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