Glutathione and cysteine levels in human tumour biopsies

Guichard, M.; Lespinasse, Françoise; R, Estelin; Gerbaulet, A.; Haie, C.; Lartigau, É.; Ep, Malaise; Micheau, C; Prade, M; Jm, Richard

doi:10.1259/0007-1285-63-751-557

Cited by 16 publications

(18 citation statements)

References 7 publications

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“…Kolanjiappan et al and Parise et al reported increasing GSH levels with tumor stage 9,10. This correlation was not found by Guichard et al6 but they limited their study to primarily T2 and T3 patients 11. Similarly, Inci et al found no difference in GSH levels when comparing patients grouped as either stage I/II or stage III 12.…”

Section: Discussionmentioning

confidence: 88%

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Basal oxidative stress ratio of head and neck squamous cell carcinomas correlates with nodal metastatic spread in patients under therapy

Dequanter¹,

Dok²,

Nuyts³

2017

OTT

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BackgroundHead and neck squamous cell carcinoma (HNSCC) is a type of cancer that is strongly associated with oxidative damage and oxidative stress. Tobacco and alcohol – sources of massive quantities of reactive oxygen species (ROS) – have been clearly identified as etiologic factors that contribute to these malignancies. Considering the role of glutathione (GSH) in ROS detoxification, we hypothesized that potential biological markers can be found in addition to the parameters of oxidative stress. In line with previous studies that emphasized the accumulation of GSH in tumor cells, in this study, we have reported a lower ratio of oxidized versus reduced GSH in head and neck tumors.ObjectiveThe aim of the paper was to evaluate the prognostic and clinical significance of the ratio of oxidized versus reduced GSH in patients with head and neck cancers.MethodsThirty-six patients with HNSCC were included in this study. The tumoral redox status was determined by measuring the ratio of oxidized/reduced GSH (GSSG/GSH) by capillary electrophoresis. Statistical analysis was performed to assess the correlation between patient, clinical factors and the redox status.ResultsThe results showed a low tumoral ratio of GSSG/GSH and a better locoregional control. Moreover, a significant correlation between the tumoral redox status ratio (GSSG/GSH) and nodal stage (N0 versus N1, N2 and N3) was also observed. A higher tumoral redox status ratio was found to be associated with the presence of lymph node metastasis (N1, N2 and N3).ConclusionA strong correlation was observed between the oxidative status and locoregional control of the tumors. Moreover, a higher basal tumoral redox status ratio was found to be correlated with the presence of lymph node metastasis.

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Section: Discussionmentioning

confidence: 88%

“…Two studies found no correlation between GSH levels and the degree of differentiation 6,7. However, Wong et al found higher levels of GSH in well differentiated oral carcinoma compared with moderately differentiated tumors 8.…”

Section: Discussionmentioning

confidence: 97%

Basal oxidative stress ratio of head and neck squamous cell carcinomas correlates with nodal metastatic spread in patients under therapy

Dequanter¹,

Dok²,

Nuyts³

2017

OTT

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“…GSH is known to be depleted in-patients exposed to carcinogen agents, such as cigarette smoke, air pollutants, occupational exposure, and viral diseases that are typically associated with cancer (3). However, elevated levels of GSH in head and neck tumors and metastatic lymphnodes were observed by Guichard et al (4). On the other hand, Peters et al (5) showed that mean value of glutathione did not differ significantly between normal, esophageal, and tumor tissues.…”

Section: Introductionmentioning

confidence: 94%

Blood glutathione levels in head and neck malignancies

Singh

Sachdeva

Aggarwal

et al. 2008

Indian J Clin Biochem

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Blood glutathione was estimated in fifty patients of head and neck cancer in the age group of 18-76 years and the results were compared with a group of normal healthy controls. Mean blood glutathione level was found to be significantly lowered in patients than the controls. Irrespective of the site, TNM classification, histopathology, and character of lesion, fall in blood glutathione was nearly same in all the patients. The mean level was significantly increased after radiotherapy when compared with the levels before radiotherapy. The decreased levels of GSH in-patients with head and neck cancer, observed in the present study, may be due to its increased utilization by the cells. The results suggest that patients with head and neck cancer have increased oxidative stress.

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“…22 In clinical studies, GSH levels were approximately 4-fold greater in oral tumor tissue than that in the surrounding ''normal'' epithelium. [23][24][25][26] In one study, increased tumor GSH levels correlated with the stage of the disease. 25 Also, increased GSH levels have been reported in cheek pouch tumors in the DMBA-induced hamster model.…”

mentioning

confidence: 96%

Enhanced levels of glutathione and protein glutathiolation in rat tongue epithelium during 4‐NQO‐induced carcinogenesis

Huang¹,

Komninou²,

Kleinman³

et al. 2007

Intl Journal of Cancer

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High glutathione (GSH) levels are commonly found in oral tumors and are thought to play an important role in tumorigenesis. While posttranslational binding of GSH to cellular proteins (protein glutathiolation) has recently been recognized as an important redoxsensitive regulatory mechanism, no data currently exist on this process during carcinogenesis. Our goal was to determine the effects of 4-nitroquinoline-N-oxide (4-NQO)-induced carcinogenesis on tongue levels of protein-bound and free GSH and related thiols in the rat. Male F-344 rats (6 weeks of age) were administered either 4-NQO (20 ppm) in drinking water or tap water alone (controls) for 8 weeks. Twenty-four weeks after cessation of 4-NQO, squamous cell carcinomas of the tongue were observed in all rats. The levels of both free and bound GSH in tumors, as well as in adjacent tissues, were 2-to 3-fold greater than in tongue epithelium from control rats (p < 0.05). Prior to tumor formation, at 8 weeks after cessation of 4-NQO, hyperplasia, dysplasia and carcinoma in situ were observed in 100%, 25% and 12.5% of 4-NQOtreated rats, respectively. At this early stage of carcinogenesis, levels of free and bound GSH were increased 50% compared with tongue tissues from control rats (p < 0.05). Glutathione disulfide (GSSG) levels were also 2-fold greater in tongue tissues from 4-NQO treated vs. control rats (p < 0.05). Altogether, these results suggest that protein glutathiolation, together with GSH and GSSG levels, are induced during oral carcinogenesis in the rat possibly as a result of enhanced levels of oxidative stress. ' 2006 Wiley-Liss, Inc.

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Glutathione and cysteine levels in human tumour biopsies

Cited by 16 publications

References 7 publications

Basal oxidative stress ratio of head and neck squamous cell carcinomas correlates with nodal metastatic spread in patients under therapy

Basal oxidative stress ratio of head and neck squamous cell carcinomas correlates with nodal metastatic spread in patients under therapy

Blood glutathione levels in head and neck malignancies

Enhanced levels of glutathione and protein glutathiolation in rat tongue epithelium during 4‐NQO‐induced carcinogenesis

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