Glutaredoxin GRXS13 plays a key role in protection against photooxidative stress in Arabidopsis

Laporte, Daniel; Olate, Ema; Salinas, Paula; Salazar, Marcela; Jordana, Xavier; Holuigue, Loreto

doi:10.1093/jxb/err301

Cited by 103 publications

(89 citation statements)

References 60 publications

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“…Despite the generation of two affinity-purified peptide antibodies targeting different regions of the AtGRXS3 protein, none of the corresponding glutaredoxin proteins could be detected by immunoblotting. This finding is consistent with the absence of protein abundance data in previous studies of class III glutaredoxins (Xing et al, 2005;Xing and Zachgo, 2008;La Camera et al, 2011;Laporte et al, 2012) and in large-scale nontargeted proteomics studies (Baerenfaller et al, 2008), likely reflecting the very low abundance of these proteins in vivo (Ströher and Millar, 2012).…”

Section: Functional Analyses Of Nitrate-regulated Glutaredoxinssupporting

confidence: 91%

“…The seven glutaredoxin genes identified as strongly and specifically induced by nitrate in our data are all class III. Only four of the 21 class III glutaredoxin genes in the Arabidopsis genome have been functionally characterized (Xing et al, 2005;Xing and Zachgo, 2008;La Camera et al, 2011;Laporte et al, 2012), and no functional information is available about any of the identified nitrate-induced glutaredoxins.…”

Section: Functional Analyses Of Nitrate-regulated Glutaredoxinsmentioning

confidence: 99%

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Nitrate-Regulated Glutaredoxins Control Arabidopsis Primary Root Growth

et al. 2015

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Nitrogen is an essential soil nutrient for plants, and lack of nitrogen commonly limits plant growth. Soil nitrogen is typically available to plants in two inorganic forms: nitrate and ammonium. To better understand how nitrate and ammonium differentially affect plant metabolism and development, we performed transcriptional profiling of the shoots of ammoniumsupplied and nitrate-supplied Arabidopsis (Arabidopsis thaliana) plants. Seven genes encoding class III glutaredoxins were found to be strongly and specifically induced by nitrate. RNA silencing of four of these glutaredoxin genes (AtGRXS3/4/5/8) resulted in plants with increased primary root length (approximately 25% longer than the wild type) and decreased sensitivity to nitratemediated inhibition of primary root growth. Increased primary root growth is also a well-characterized phenotype of many cytokinin-deficient plant lines. We determined that nitrate induction of glutaredoxin gene expression was dependent upon cytokinin signaling and that cytokinins could activate glutaredoxin gene expression independent of plant nitrate status. In addition, crosses between "long-root" cytokinin-deficient plants and "long-root" glutaredoxin-silenced plants generated hybrids that displayed no further increase in primary root length (i.e. epistasis). Collectively, these findings suggest that AtGRXS3/4/5/8 operate downstream of cytokinins in a signal transduction pathway that negatively regulates plant primary root growth in response to nitrate. This pathway could allow Arabidopsis to actively discriminate between different nitrogen sources in the soil, with the preferred nitrogen source, nitrate, acting to suppress primary root growth (vertical dimension) in concert with its well-characterized stimulatory effect on lateral root growth (horizontal dimension).

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Section: Functional Analyses Of Nitrate-regulated Glutaredoxinssupporting

confidence: 91%

Section: Functional Analyses Of Nitrate-regulated Glutaredoxinsmentioning

confidence: 99%

Nitrate-Regulated Glutaredoxins Control Arabidopsis Primary Root Growth

et al. 2015

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“…Nine of the 30 glutaredoxin genes in the Arabidopsis genome were identified as being up-regulated by cytokinin. The functions of glutaredoxins in plants are only beginning to be elucidated (Rouhier et al, 2008), but include roles in the protection against photooxidative stress (Laporte et al, 2012), ironsulfur cluster assembly, flower development (Wang et al, 2009), and pathogen response (Wang et al, 2009). The cytokinin regulation of multiple glutaredoxin genes suggests a link to these processes.…”

Section: Differential Expression Analysis Of Rna-seq Datamentioning

confidence: 99%

“…Rather than create a new gene model 15 , AT5G05860 (UGT76C2) 16 , AT5G56970 (CKX3) 17 , AT2G17820 (AHK1) 18 21 , AT1G74890 (ARR15) 22 , AT1G59940 (ARR3) 23 , AT1G19050 (ARR7) 23 , AT3G48100 (ARR5) 23 , AT3G57040 (ARR9) 23 , AT5G62920 (ARR6) 23 , AT2G41310 (ARR8) 23 , AT2G28160 (bHLH029) 24 , AT2G22770 (NAI1) 25 , AT4G26150 (CGA1) 26,27 , AT1G10480 (ZFP5) 28,29,30 , AT1G16530 (ASL9) 31 , AT1G04240 (SHY2) 13 , AT1G72360 (ERF73) 32 , AT2G40970 (MYBC1) 33 , AT1G71030 (MYBL2) 34 , AT5G65210 (TGA1) 35 , AT2G21650 (MEE3), AT4G39070 (B-box zinc finger), AT5G50915 (bHLH), AT4G23750 (CRF2), AT2G40670 (ARR16), AT1G10470 (ARR4), AT4G25410 (bHLH), AT1G18400, AT1G04250 (BEE1), AT2G27840 (HDT4), AT1G67030 (ZFP6), AT1G68360 (C2H2), AT3G48920 (bHLH), AT5G05790 (Homeodomain), AT3G04070 (ANAC047), AT1G68880 (ZIP), AT5G15830 (ZIP3), AT1G31320 (LBD4), AT3G50700 (IDDT2) 1 Ralph et al (2007). 3 Laporte et al (2012). 4 Wang et al (2009).…”

Section: New Gene Models and Alternative Splicing In Genes Involved Imentioning

confidence: 99%

Identification of Cytokinin-Responsive Genes Using Microarray Meta-Analysis and RNA-Seq in Arabidopsis

et al. 2013

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Cytokinins are N 6 -substituted adenine derivatives that play diverse roles in plant growth and development. We sought to define a robust set of genes regulated by cytokinin as well as to query the response of genes not represented on microarrays. To this end, we performed a meta-analysis of microarray data from a variety of cytokinin-treated samples and used RNA-seq to examine cytokininregulated gene expression in Arabidopsis (Arabidopsis thaliana). Microarray meta-analysis using 13 microarray experiments combined with empirically defined filtering criteria identified a set of 226 genes differentially regulated by cytokinin, a subset of which has previously been validated by other methods. RNA-seq validated about 73% of the up-regulated genes identified by this meta-analysis. In silico promoter analysis indicated an overrepresentation of type-B Arabidopsis response regulator binding elements, consistent with the role of type-B Arabidopsis response regulators as primary mediators of cytokinin-responsive gene expression. RNA-seq analysis identified 73 cytokinin-regulated genes that were not represented on the ATH1 microarray. Representative genes were verified using quantitative reverse transcription-polymerase chain reaction and NanoString analysis. Analysis of the genes identified reveals a substantial effect of cytokinin on genes encoding proteins involved in secondary metabolism, particularly those acting in flavonoid and phenylpropanoid biosynthesis, as well as in the regulation of redox state of the cell, particularly a set of glutaredoxin genes. Novel splicing events were found in members of some gene families that are known to play a role in cytokinin signaling or metabolism. The genes identified in this analysis represent a robust set of cytokininresponsive genes that are useful in the analysis of cytokinin function in plants.

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“…Several Arabidopsis (Arabidopsis thaliana) GRXs from class III participate in the tolerance to photooxidative stress (Laporte et al, 2012) and defense against pathogens (Ndamukong et al, 2007;La Camera et al, 2011). Others are required for proper reproductive development through interaction with basic Leu zipper-type TGA transcription factors (Xing and Zachgo, 2008;Murmu et al, 2010).…”

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confidence: 99%