Glutaraldehyde‐treated Bovine Pericardium: Effects of Lyophilization on Cytotoxicity and Residual Aldehydes

Abstract

This work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentration of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular growth. Residual aldehydes were measured by HPLC. Although both groups' eluants exhibited some cytotoxicity, the eluant from group A was less cytotoxic, with a cytotoxicity index (IC50(%)) of 41%. Group B eluants all had marked cytotoxic effects; cell growth was 24.15% of the negative control at the most dilute eluant concentration (6.25%). The mean residual glutaraldehyde level was less in group A than in group B (2.36 +/- 0.11 and 9.90 +/- 3.70 g/l, respectively; n=3, P < 0.05), but residual formaldehyde levels did not differ. These results demonstrate that compared with conventional glutaraldehyde-treated bovine pericardium, lyophilized pericardium is less cytotoxic, with fewer glutaraldehyde residues.

Mistura de proteínas morfogenéticas ósseas, hidroxiapatita, osso inorgânico e colágeno envolta por membrana de pericárdio no preenchimento de defeito ósseo segmentar em coelhos

“…A eficácia do uso de membrana está relacionada à capacidade de atuação como barreira física, prevenindo a invasão do espaço do defeito por tecido mole circunjacente (Lekovic et al, 2001). Em geral, as membranas de colágeno durante o preparo necessitam de tratamento por agente que promova o aparecimento de ligações cruzadas entre o colágeno, aumentando a resistência à degradação enzimática (Cirelli et al, 1997;Maizato et al, 2003). A membrana usada no experimento foi liofilizada e esterilizada com raio gama, contudo não houve tratamento para a produção de ligações cruzadas.…”

“…A membrana usada no experimento foi liofilizada e esterilizada com raio gama, contudo não houve tratamento para a produção de ligações cruzadas. Embora esses agentes sejam muitas vezes citotóxicos (Maizato et al, 2003), a não utilização pode favorecer a rápida reabsorção do material, como notado por James et al (1991), ao utilizarem o pericárdio bovino sem ligação cruzada no reparo da parede abdominal de coelhos.…”

Comparação entre o pericárdio bovino decelularizado e o pericárdio bovino convencional utilizado na confecção de biopróteses valvares cardíacas

“…Of all factors currently considered as being responsible for prosthetic degeneration, by far, those which are the most important and are the most studied are calcification and the toxicity of glutaraldehyde [19][20][21][22][23]. Among the mechanisms involved in calcification, there is evidence that the presence of dead cells and cellular debris incompletely fixed by the treatment methods, work as calcification nuclei, permitting the deposition of calcium, sodium and magnesium phosphate and carbonate crystals [23].…”

Freeze-drying as an alternative method of human sclera preservation

“…This alternative technique has been used to preserve many biological products such as bovine pericardium (9,11) , human red blood cells (12) , epidermal keratinocytes (13) , amniotic membrane (14) , vaccines (15) and others.…”