Glutaraldehyde-polymerized hemoglobin and tempol (PolyHb-tempol) has superoxide dismutase activity that can attenuate oxidative stress on endothelial cells induced by superoxide anion

Wu, Mengdi; Feng, Kun; Li, Qiuhui; Ma, Huiya; Zhu, Hongli; Xie, Yudou; Yan, Gaofei; Chen, Chao; Yan, Kunping

doi:10.1080/21691401.2017.1328685

Cited by 11 publications

(12 citation statements)

References 34 publications

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“…investigated the synthesis of niosomes containing quercetin, doxorubicin, and siRNA. The observation of the optical microscope has revealed multi-layered spheres, whose formation and morphology are similar to our findings (32). One of the most experimental approaches used to determine the formation of drug delivery carriers is size distribution measurement.…”

Section: Discussionsupporting

confidence: 90%

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In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents

et al. 2019

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The low solubility of the plant-extracted agent like D-limonene in cancer therapy is a critical problem. In this study, we prepared D-limonene-loaded niosomes (D-limonene/Nio) for cancer therapy through in vitro cytotoxicity assay of HepG2, MCF-7, and A549 cell lines. The niosomal formulation was prepared by film hydration technique with Span® 40: Tween® 40: cholesterol (35:35:30 molar ratio) and characterized for vesicle distribution size, morphology, entrapment efficiency (EE%), and in vitro release behaviour. The obtained niosomes showed a nanometric size and spherical morphology with EE% about 87 ± 1.8%. Remarkably prolonged release of D-limonene from niosomes compared to free D-limonene observed. The loaded formulation showed significantly enhanced cytotoxic activity with all three cancer cell lines (HepG2, Macf-7 and A549) at the concentration of 20 μM. These results indicated that niosome loaded with phytochemicals can be a promising nano-carrier for cancer therapy applications

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Section: Discussionsupporting

confidence: 90%

“…The loaded niosome formulation had a controlled release rate and released the content within 12 h. Hemati et al . reported, under the normal temperature and pH of the body, minimum release rates for doxorubicin, 27.4% and 45.3% for quercetin, after 72 h (32). Ismail et al .…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents

et al. 2019

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“…High concentrations of low MW PolyHb, when used at high doses, induce coronary microcirculation toxicity due to oxidative stress [ 36 ]. Newer generations of HBOCs are coupled with antioxidants to minimize free radical formation [ 37 ]. The dramatic decrease in auto-oxidation of PolyHb and the precursor Hb solution compared to pure Hb likely results from copolymerization of superoxide dismutase and catalase that is present in the precursor Hb solution [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Polymerized human hemoglobin increases the effectiveness of cisplatin-based chemotherapy in non-small cell lung cancer

et al. 2020

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Cisplatin is a promising therapeutic for the treatment of non-small cell lung cancer (NSCLC). Unfortunately, a significant portion of NSCLC patients relapse due to cisplatin chemoresistance. This chemoresistance is thought to be primarily associated with hypoxia in the tumor microenvironment. Administration of hemoglobin (Hb)-based oxygen (O 2 ) carriers (HBOCs) is a promising strategy to alleviate hypoxia in the tumor, which may make cisplatin more effective. In this study, we administered a high O 2 affinity, relaxed state (R-state) polymerized hemoglobin (PolyHb) to three different NSCLC cell lines cultured in vitro and implanted in vivo into healthy mice. The R-state PolyHb administered in this study is unable to deliver O 2 unless under severe hypoxia which significantly limits its oxygenation potential. In vitro sensitivity studies indicate that the administration of PolyHb increases the effectiveness of cisplatin under hypoxic conditions. Additional animal studies revealed that co-administration of PolyHb with cisplatin attenuated tumor growth without alleviating hypoxia. Analysis of reactive O 2 species production in the presence of hypoxic culture indicates that exogenous ROS production by oxidized PolyHb may the mechanism of chemosensitization. This ROS mechanism, coupled with oxygenation, may be a potential chemosensitizing strategy for use in NSCLC treatment.

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“…In order to avoid the excessive molecular weight of crosslinking products, glycine should be added to neutralize some of the GA, but the supplementary method is difficult to operate in actual production. However, if the preparation of large molecular weight cross-linked Hb, for example: OxyVita(V R )Hb [33][34][35], and in this process a lot of GA can be added, GA may effectively inactivate PPV during the polymerization of high-molecular-weight HBOCs.…”

Section: Discussionmentioning

confidence: 99%

Glutaraldehyde inactivation of enveloped DNA viruses in the preparation of haemoglobin-based oxygen carriers

Feng

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Glutaraldehyde (GA), used medically as a disinfectant and as a crosslinker for haemoglobin (Hb)-based oxygen carriers (HBOCs), was investigated for its ability to inactivate viruses during the preparation of these artificial blood substitutes. Porcine parvovirus (PPV; a non-enveloped DNA virus) and porcine pseudorabies virus (PRV; an enveloped DNA virus) were used as the virus indicators. Upon treatment with 0.1 mM GA, the titer of PRV decreased from 9.62 log to 2.62 log within 0.5 h, whereas that of PPV decreased from 7.00 log to 2.30 log in 5 h. Following treatment with 1.0 mM GA, the titer of PRV decreased from 11.00 log to 1.97 log within 0.5 h, whereas that of PPV decreased from 7.50 log to 3.43 log in 4.5 h. During the polymerization of Hb with GA, the GA concentration decreased to 1.0 and 0.1 mM within 30 and 50 min, respectively, at a GA:Hb molar ratio of 10:1, whereas at a GA:Hb molar ratio of 30:1, GA decreased to those same concentrations in 1.5 and 2.5 h, respectively. This rapid decrease in GA concentration during its polymerization with Hb indicates that GA must be added into the Hb solution in a short time in order to get as high a initial concentration as possible. In this study, the GA can only inactivate PRV effectively, given that a longer time (4.5 h) was required for it to inactivate the PPV titer. This study therefore demonstrates that GA inactivates the enveloped DNA virus only during the preparation of HBOCs.

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Glutaraldehyde-polymerized hemoglobin and tempol (PolyHb-tempol) has superoxide dismutase activity that can attenuate oxidative stress on endothelial cells induced by superoxide anion

Cited by 11 publications

References 34 publications

In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents

In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents

Polymerized human hemoglobin increases the effectiveness of cisplatin-based chemotherapy in non-small cell lung cancer

Glutaraldehyde inactivation of enveloped DNA viruses in the preparation of haemoglobin-based oxygen carriers

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