Glutamine Uptake by Cortical Slices and Luminal Brush Border Vesicles of Rat Kidney

Alleyne, George A.O.; Anderson, Norma McFarlane; Scott, B.W.

doi:10.1016/b978-0-08-025517-0.50025-4

Cited by 3 publications

(2 citation statements)

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“…The transport of neutral amino acids in brush-border membranes prepared from the kidney cortex of several species has been studied in detail (Evers et al, 1976;Fass et al, 1977; McFarlane-Anderson & Alleyne, 1979;Goldman et al, 1979;McNamara et al, 1979McNamara et al, , 1981Alleyne et al, 1980;Mircheff et al, 1982;Medow et al, 1983;Biber et al, 1983;Steiger et al, 1983). It is found that in all cases neutral amino acid transport is to a large extent dependent on an electrochemical gradient of Na+ ions.…”

Section: Introductionmentioning

confidence: 99%

A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes

Lynch

McGivan

1987

Biochemical Journal

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1. A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide ('MEGA-10') was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins.

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Section: Introductionmentioning

confidence: 99%

A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes

Lynch

McGivan

1987

Biochemical Journal

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“…can be used to derive a qualitative idea about the utilization of the substrate, as the modulation studies done in this paper indi cate. The higher rates of oxidation observed when glutamine was the only substrate in the incubation medium compared to the supple mented medium may be related in part to the decline in the uptake of glutamine when other amino acids are present [ 17,18].…”

Section: Discussionmentioning

confidence: 99%

Tubular CO<sub>2</sub> Production from Glutamine in the Rat: Segmental Profile and Modulation

Mujais¹,

Zahid²

1992

Kidney Blood Press Res

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The present study was designed to test whether tubular carbon dioxide production from the carbon skeleton of uniformly ¹⁴C-labelled glutamine exhibits quantitative and qualitative segmental heterogeneity. Our results show that CO₂ production from glutamine in the proximal convoluted tubule (PCT) was dependent on substrate concentrations and is saturable at 10^-4M of glutamine. Glutamine oxidation was demonstrable in all nephron segments examined. The PCT is the quantitatively predominant site of glutamine oxidation. Intermediate nephron segments, however, such as the thick ascending limb (MAL) and the distal convoluted tubule possess a significant capacity for glutamine oxidation, particularly when examined in terms of tubular protein content. Modulation of glutamine oxidation by extracellular pH was segment specific. Stimulation by acidosis and inhibition by alkalosis were observed in the PCT while carbon dioxide production from glutamine in the MAL was pH-insensitive. Glutamine oxidation was closely linked to sodium transport and greatly decreased by inhibition of Na-K-ATPase. In both the PCT and MAL, glutamine oxidation was inhibited by high extracellular potassium concentrations and in the PCT enhanced by extracellular hypokalemia. N-Ethyl maleiamide, an inhibitor of proton ATPase, led to almost complete cessation of CO₂ production from the substrate in both PCT and MAL. Acetazolamide, an inhibitor of carbonic anhydrase, led to a partial reduction in carbon dioxide formation in the PCT, but did not affect glutamine oxidation in the MAL. We conclude that segmental qualitative heterogeneity characterizes oxidation of the carbon skeleton of glutamine with proximal segments showing the predictable effects of pH changes and carbonic anhydrase inhibition. The MAL appears to be nonmodulating.

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Glutamine

Kvamme

1983

Metabolism in the Nervous System

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Glutamine Uptake by Cortical Slices and Luminal Brush Border Vesicles of Rat Kidney

Cited by 3 publications

References 14 publications

A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes

A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes

Tubular CO<sub>2</sub> Production from Glutamine in the Rat: Segmental Profile and Modulation

Glutamine

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