Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus

Chungopast, Sirinapa; Thapanapongworakul, Pilunthana; Matsuura, Hiroyuki; Dao, Tan Van; Asahi, Toshimasa; Tada, Kuninao; Tajima, Shigeyuki; Nomura, Masatoshi

doi:10.1016/j.jplph.2013.10.015

Cited by 10 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1–3; Appendix S1), and (3) maintained intact symbiosomes with relatively stable bacteroid populations (Table 3). These latter features are typical of effective nodules in legumes with determinate nodule development (Chua et al, 1985; Banba et al, 2001; Schumpp and Deakin, 2010; Thapanapongworakul et al, 2010; Chungopast et al, 2014). The features associated with the hypothesized induced cellular senescence appeared to initiate slightly earlier in L. japonicus hosts (∼5 wpi) compared to A. strigosus (∼6 wpi) consistent with the early‐flowering phenotype and faster development of the L. japonicus MG‐20 accession (Kawaguchi, 2000).…”

Section: Discussionmentioning

confidence: 89%

“…Moreover, induced senescence shares key features of programmed cell death in plants. Programmed cell death is initiated when the tonoplast surrounding the vacuole ruptures, which releases enzymes that degrade the cytoplasmic contents, nuclei, and organelles (van Doorn, 2011), similar to what has been observed in nodules with ineffective rhizobia (Chua et al, 1985; Banba et al, 2001; Schumpp and Deakin, 2010; Chungopast et al, 2014).…”

mentioning

confidence: 75%

“…For legumes with determinate nodules, a potential mechanism for legume sanctions derives from histological observations that nodules with ineffective rhizobia undergo an accelerated schedule of nodule senescence (Chua et al, 1985; Banba et al, 2001; Schumpp and Deakin, 2010; Chungopast et al, 2014). Nodule senescence is a natural process by which the nodule organ breaks down (Thomas, 2013), and two potential triggers have been described.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Cell autonomous sanctions in legumes target ineffective rhizobia in nodules with mixed infections

Regus

Quides

O’Neill

et al. 2017

American J of Botany

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 75%

mentioning

confidence: 99%

See 1 more Smart Citation

Cell autonomous sanctions in legumes target ineffective rhizobia in nodules with mixed infections

Regus

Quides

O’Neill

et al. 2017

American J of Botany

View full text Add to dashboard Cite

show abstract

“…The mutant is significantly reduced in its ability to fix nitrogen, as measured by acetylene reduction (ca. 45%, [ 62 ]). Glutamine synthetase (along with glutamine synthase) is responsible for the assimilation of ammonium in legume nodules, which is the main product of nitrogen fixation [ 62 ].…”

Section: Introductionmentioning

confidence: 99%

Lotus japonicus alters in planta fitness of Mesorhizobium loti dependent on symbiotic nitrogen fixation

et al. 2017

View full text Add to dashboard Cite

Rhizobial bacteria are known for their capacity to fix nitrogen for legume hosts. However ineffective rhizobial genotypes exist and can trigger the formation of nodules but fix little if any nitrogen for hosts. Legumes must employ mechanisms to minimize exploitation by the ineffective rhizobial genotypes to limit fitness costs and stabilize the symbiosis. Here we address two key questions about these host mechanisms. What stages of the interaction are controlled by the host, and can hosts detect subtle differences in nitrogen fixation? We provide the first explicit evidence for adaptive host control in the interaction between Lotus japonicus and Mesorhizobium loti. In both single inoculation and co-inoculation experiments, less effective rhizobial strains exhibited reduced in planta fitness relative to the wildtype M. loti. We uncovered evidence of host control during nodule formation and during post-infection proliferation of symbionts within nodules. We found a linear relationship between rhizobial fitness and symbiotic effectiveness. Our results suggest that L. japonicus can adaptively modulate the fitness of symbionts as a continuous response to symbiotic nitrogen fixation.

show abstract

“…O N2 fixado pela nitrogenase e transformado em NH3 é protonado em NH4 + e exportado para o citossol da célula infectada pelo nódulo através da membrana do simbionte (UDVARDI; POOLE, 2013). Uma vez no citossol das células do nódulo infectado, o NH4 + é enzimaticamente convertido em glutamina (Gln) pela glutamina sintase (GS), (CHUNGOPAST et al, 2014;LOTHIER et al, 2011). Os níveis elevados de glutamina nos plastídios estimulam a ativação da glutamato sintase (GOGAT), produzindo duas moléculas de glutamato.…”

Section: Fixação Biológica De Nitrogêniounclassified

Pré-inoculação e tratamento industrial de sementes: efeito na fixação biológica de nitrogênio, no desenvolvimento e na produtividade da soja

Engroff¹

View full text Add to dashboard Cite

A Deus, pela vida, pelas oportunidades e por iluminar minha caminhada. Aos meus pais, Berti e Delci, obrigada pelas palavras de apoio nos momentos difíceis, por nunca me deixarei desistir, por serem minha maior fonte de inspiração, vocês são meus pilares. Nem todas as palavras são capazes de expressa a gratidão que sinto por vocês. Amo vocês! Aos meus irmãos, Larissa e Rodrigo, pelo companheirismo, pela paciência comigo, pelos momentos de risada e por apesar da distância nunca termos perdido essa conexão que existe entre nós! Amo vocês com todo meu coração. Ao meu orientador Prof. Dr. Durval Dourado Neto, por me aceitar como sua orientada, por todo o apoio, conhecimentos transmitidos, pelos conselhos e experiência compartilhadas e pela paciência durante esse caminho. Ao meu grande amigo Felipe Fadel Sartori, pela amizade construída, pelos conselhos, pela paciência, pela colaboração no trabalho, conhecimentos transmitidos, por sempre estar me incentivando a crescer. As palavras escritas aqui representam muito pouco perto de tudo que você fez por mim. Ao meu Grande amigo Guilherme Felisberto, pelos momentos de descontração, conhecimentos transmitidos, pela colaboração no trabalho, pela ajuda diária, pelo apoio incondicional, pela amizade, meu muito obrigada.

show abstract

Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus

Cited by 10 publications

References 24 publications

Cell autonomous sanctions in legumes target ineffective rhizobia in nodules with mixed infections

Cell autonomous sanctions in legumes target ineffective rhizobia in nodules with mixed infections

Lotus japonicus alters in planta fitness of Mesorhizobium loti dependent on symbiotic nitrogen fixation

Pré-inoculação e tratamento industrial de sementes: efeito na fixação biológica de nitrogênio, no desenvolvimento e na produtividade da soja

Contact Info

Product

Resources

About