Glutamine supplementation improves frozen-thawed beluga sturgeon, <i>Huso huso</i>
 (Linnaeus, 1758) semen

Aramli, Mohammad Sadegh; Nazari, Rajab Mohammad

doi:10.1111/jai.13052

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…Semen motility was recorded with dark‐field microscopy (400×, Olympus CK2, Tokyo, Japan). The percentage motility was determined arbitrarily on a 0‐ to 4‐point scale: 0, when no movement was observed; 1, when up to 25% cells were moving; 2, when up to 50% cells were moving; 3, when up to 75% cells were moving; and 4, when more than 75% cells were moving (Aramli & Nazari, ; Viveiros, Jatzkowski, & Komen, ). The duration of motility was determined by recording the time taken from activation to the complete cessation of activity by the last semen in a field.…”

Section: Methodsmentioning

confidence: 99%

Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels

Hajiahmadian

Moghanlou

Agh

et al. 2016

Reprod Domestic Animals

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Brood fish nutrition is an important factor susceptible to affect not only fecundity and gametogenesis but also gamete quality. In this study, we investigated the effects of altering dietary vegetable fatty acid content on semen quality (i.e. motility, density and seminal plasma composition), fertilizing ability and also blood testosterone (T) concentration in rainbow trout (Oncorhynchus mykiss). Fish were fed a commercial diet and ten formulated diets with similar proximate compositions but different levels of vegetable fatty acids (highly unsaturated fatty acids (HUFA): monounsaturated fatty acids (MUFA); HUFA: polyunsaturated fatty acids (PUFA); and HUFA: saturated fatty acids (SFA) ratios). Fish fed with HUFA: MUFA = 0.0 and HUFA: SFA = 0.25 ratios had the highest semen motility percentage and duration. However, the highest semen concentration and semenatocrit were observed in HUFA: SFA = 0.0 and HUFA: PUFA = 0.37 ratios. There was a significant difference in terms of K ion among diets supplemented with HUFA: PUFA = 0.0, HUFA: PUFA = 0.37 and HUFA: MUFA = 0.16 ratios (p < .05). Furthermore, Na ion showed significant difference between control group and diet supplemented with HUFA: PUFA = 0.0 (p < .05). Among the biochemical parameters, total protein showed a significant difference between HUFA: MUFA = 0.16 and HUFA: PUFA = 0.37 ratios (p < .05). No significant differences in fertilization ability and blood T concentration were found among dietary treatments (p > .05). In addition, the present data suggest that dietary fatty acid levels could affect semen quality but not fertilization ability in O. mykiss.

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Section: Methodsmentioning

confidence: 99%

Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels

Hajiahmadian

Moghanlou

Agh

et al. 2016

Reprod Domestic Animals

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“…Determination of the best concentration and combination of amino acids for supplementation are important for the success of cryopreservation (Aramli et al, 2016). Studies show improvements in sperm quality with the use of amino acids in semen of fish (Aramli et al, 2016; Aramli & Nazari, 2016; Martínez‐Páramo et al, 2013; Öğretmen et al, 2015). However, studies have reported that a high concentration of amino acids negatively affected sperm quality due to toxicity (Kaeoket et al, 2010; Khlifaoui et al, 2005; Kledmanee et al, 2013; Li et al, 2018; Trimeche et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Glutamine, for example, is the main free amino acid found in seminal plasma of mammals (Aramli et al, 2016) and are known to play an important biological role in preventing cell damage during cryopreservation (Aramli et al, 2016; Cabrita et al, 2011; Rani, & Munuswamy, 2014; Valdebenito et al, 2010). Several authors have reported that glutamine supplementation in the cryoprotectant solution can improve sperm motility and post‐thaw fertilization potential of bovine spermatozoa (Amirat‐Briand et al, 2009), goats (Bucak et al, 2010), sheep (Bucak et al, 2009) and fish (Aramli et al, 2016; Aramli & Nazari, 2016). Since glutamine is a precursor of glutathione, its supplementation can elevate the levels of glutathione in seminal plasma avoiding the oxidative stress damage (Cruzat et al, 2009; Tuncer et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish

Costa

Marques

Lassen

et al. 2020

Aquaculture Research

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The aim of this study was to investigate the effects of different concentrations of glutamine (experiment 1) and glutamine + cysteine (experiment 2) on post‐thaw sperm quality of South American silver catfish (Rhamdia quelen). The sperm was cryopreserved in cryoprotectant solution (fructose 50 g/L, powdered milk 50 g/L and methanol 100 ml/L) containing different glutamine concentrations (0, 2.5 and 5 mM; experiment 1) and a combination of glutamine (2.5 and 5 mM) and cysteine (2.5 and 5 mM; experiment 2). In experiment 1: glutamine supplementation groups increased DNA fragmentation; the concentration of carbonyl groups was lower and the concentration of sulfhydryl groups was higher in control; a higher activity of superoxide dismutase (SOD), catalase (CAT), glutathione‐S‐transferase (GST) and glutathione peroxidase (GPx) enzymes was observed in the 2.5 and 5 mM groups. In experiment 2: the group with 5 mM of cysteine +5 mM of glutamine presented the higher DNA damage; treatments with 5 mM of glutamine presented the higher concentration of carbonyl groups and a higher activity of CAT and GST. The findings of this study show that glutamine and cysteine, at the concentrations evaluated in this study, does not present satisfactory results but rather damaging effects on sperm quality of R. quelen.

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“…More recently, various studies have been performed on different fish species to examine the benefits of such antioxidant properties and investigate the effect of adding amino acids to semen extenders (Table 4). Such works focused on A. baerii and A. ruthenus (Stejskal et al., 2008), O. mykiss (Ekici et al., 2012), H. huso (Aramli & Nazari, 2016) and C. carpio (Kanyilmaz & İnanan, 2020). Motility, membrane stability and semen functionality in fish, similar to mammals, are also affected by oxidative stress due to the generation of reactive oxygen species during dilution in the extender media and cooling process.…”

Section: Non‐permeable Cryoprotectant Agentsmentioning

confidence: 99%

“…Based on the data in Tables 1–4, the effects of permeable and non‐permeable cryoprotectants have been extensively studied for the cryopreservation of fish (e.g. Abed‐Elmdoust et al., 2015; Aramli, Golshahi, Nazari, Aramli, & Banan, 2015; Aramli & Nazari, 2014, 2016; Golshahi et al., 2018; Judycka et al., 2021; Lopes et al., 2022; Rahimi et al., 2015) semen. The other studies have focused on the cryopreservation of mammal semen (e.g.…”

Section: Introductionmentioning

confidence: 99%

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

Aramli,

Sarvi Moghanlou,

Pourahad Anzabi

2024

Reprod Domestic Animals

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Cryopreservation is a valuable technique used to assist in the genetic improvement of cultured stocks and provide a continuous supply of good‐quality semen for artificial insemination. Conserving semen by cryopreservation serves several purposes (e.g. artificial reproductive technologies and species conservation) and is also used in the clinical treatment of human infertility. However, the lifespan of cryopreserved semen is influenced by a range of factors, including storage temperature, cooling rate, chemical composition of the extender, the concentration of cryoprotectant, reactive oxygen species, seminal plasma composition and hygienic control. The choice of cryoprotectant is a vital factor underlying the success of animal semen cryopreservation. In this regard, extensive research has been carried out on various cryoprotectants, such as egg yolk, dimethyl sulfoxide, methanol, ethylene glycol and dimethylacetamide. Recent studies have also described the use of a range of new cryoprotectants for cryopreservation, including compounds of plant origin (soy), amino acids, antifreeze proteins, carbohydrates and cyclodextrins. Moreover, semen cryopreservation and storage require the use of liquid nitrogen or ultralow refrigeration methods for both long‐ and short‐term storage. This review summarizes the general methods used for freezing semen and discusses the use of traditional and newly emerging cryoprotectants (permeable and non‐permeable) for the cryopreservation of semen in selected fish and mammalian species.

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Glutamine supplementation improves frozen-thawed beluga sturgeon, Huso huso (Linnaeus, 1758) semen

Cited by 5 publications

References 20 publications

Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels

Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels

Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

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