Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice

Wu, Dan; Su, Sen; Zha, Xule; Yan, Wei; Yang, Gang; Huang, Qianyi; Yang, Yongjun; Lin, Xia; Fan, Shijun; Peng, Xi

doi:10.1016/j.redox.2022.102581

Cited by 13 publications

(10 citation statements)

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“…Whereas the impact of Gln treatment on ISCs has been reported only in very few studies [ 20 ]. Gln has an antioxidant function, which plays an important role in maintaining the redox balance of the body [ 8 , 21 ]. This study verified that Gln treatment decreased small intestinal injury scores, mitigated pathological injury, increased the length of the crypt, relieved crypt atrophy, and improved the survival of the crypts of small intestinal.…”

Section: Discussionmentioning

confidence: 99%

“…As described in previous study [ 21 ], we made tissue sections using small intestine tissue and performed H&E staining. Small intestine tissues of mice at different time points were taken, and the ileum was fixed with 4% paraformaldehyde.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the previous study [ 21 ], 200 μL Radioimmunoprecipitation (RIPA) solution (Beyotime, Shanghai, China), which contains 1x protease inhibitor, was added to crack the crypts and shaken at 4 °C for 20 min to fully crack the crypts. The protein concentration was determined by Bicinchoninic Acid (BCA) kit (Beyotime, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…As described in previous study [ 21 ], paraffin sections of mouse small intestine were used for immunofluorescence analysis. The paraffin sections were first baked at 60 °C for 2 h, then dewaxed and cleaned with PBS.…”

Section: Methodsmentioning

confidence: 99%

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Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study

Chen

Zhang

et al. 2023

Nutrients

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Burn injury is a common form of traumatic injury that leads to high mortality worldwide. A severe burn injury usually induces gut barrier dysfunction, partially resulting from the impairment in the proliferation and self-renewal of intestinal stem cells (ISCs) post burns. As a main energy substance of small intestinal enterocytes, glutamine (Gln) is important for intestinal cell viability and growth, while its roles in ISCs-induced regeneration after burns are still unclear. To demonstrate the potential effects of Gln in improving ISCs proliferation and alleviating burn-induced intestinal injury, in this study, we verified that Gln significantly alleviated small intestine injury in burned mice model. It showed that Gln could significantly decrease the ferroptosis of crypt cells in the ileum, promote the proliferation of ISCs, and repair the crypt. These effects of Gln were also confirmed in the mouse small intestine organoids model. Further research found that Yes-associated protein (YAP) is suppressed after burn injury, and Gln could improve cell proliferation and accelerate the renewal of the damaged intestinal mucosal barrier after burns by activating YAP. YAP is closely associated with the changes in intestinal stem cell proliferation after burn injury and could be served as a potential target for severe burns.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study

Chen

Zhang

et al. 2023

Nutrients

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“…Glutamine (Gln) is a precursor for the synthesis of nucleotides, glucose, and amino acids and the major metabolic fuel used by intestinal cells . Dietary supplementation of Gln to weaned piglets and mice can improve growth performance and intestinal morphology by reducing oxidative damage, stimulating intestinal cell proliferation, regulating cell survival and death, and enhancing intestinal paracellular permeability. − Moreover, in vivo and in vitro studies have shown that Gln maintains intestinal barrier integrity by improving intestinal morphology and participating in the regulation of barrier function, tight junction protein expression, and immune-related gene expression. , In addition, a prior study showed that Gln can activate the Wnt/β-catenin pathway to accelerate the proliferation of pancreatic stellate cells . Gln starvation treatment results in Wnt/β-catenin pathway dysfunction and weakened protein synthesis .…”

Section: Introductionmentioning

confidence: 99%

Glutamine Promotes Porcine Intestinal Epithelial Cell Proliferation through the Wnt/β-Catenin Pathway

Fang,

Lu,

Cheng

et al. 2024

J. Agric. Food Chem.

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Glutamine (Gln) is a critical nutrient required by neonatal mammals for intestinal growth, especially for newborn piglets. However, the mechanisms underlying the role of Gln in porcine intestinal epithelium development are not fully understood. The objective of the current study was to explore the possible signaling pathway involved in the promotion of porcine intestinal epithelial cell (IPEC-J2) proliferation by Gln. The results showed that 1 mM Gln promoted IPEC-J2 cell proliferation, and tandem mass tag proteomics revealed 973 differentially expressed proteins in Gln-treated IPEC-J2 cells, 824 of which were upregulated and 149 of which were downregulated. Moreover, gene set enrichment analysis indicated that the Wnt signaling pathway is activated by Gln treatment. Western blotting analysis further confirmed that Gln activated the Wnt/β-catenin signaling pathway. In addition, Gln increased not only cytosolic β-catenin but also nuclear β-catenin protein expression. LF3 (a β-catenin/TCF4 interaction inhibitor) assay and β-catenin knockdown demonstrated that Gln-mediated promotion of Wnt/β-catenin signaling and cell proliferation were blocked. Furthermore, the inhibition of TCF4 expression suppressed Gln-induced cell proliferation. These findings further confirmed that Wnt/β-catenin signaling is involved in the promotion of IPEC-J2 cell proliferation by Gln. Collectively, these findings demonstrated that Gln positively regulated IPEC-J2 cell proliferation through the Wnt/β-catenin pathway. These data greatly enhance the current understanding of the mechanism by which Gln regulates intestinal development.

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O-GlcNAcylation Modification in Immune Cells: Potential Therapeutic Implications of Sepsis

Huang¹,

2023

Intensive Care Res

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O-GlcNAc modification is a glycosylation modification that is commonly observed in nuclear and organelle proteins of cells. In recent years, several studies have shown that O-GlcNAc modification plays an important regulatory role in immune cells, which regulates their activity and function and inhibits inflammatory responses. It also enhances immune cells recognition and clearance of pathogens, and improves the host´s antibacterial immune response. Sepsis is a systemic inflammatory response to infection, whose development and progression are regulated by the immune system. therefore. In the present review, we will discuss the mechanism of O-GlcNAc modification in immune cells and its potential therapeutic value in sepsis, which will be expected to provide new insights and targets for the treatment of sepsis.

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Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice

Cited by 13 publications

References 74 publications

Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study

Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study

Glutamine Promotes Porcine Intestinal Epithelial Cell Proliferation through the Wnt/β-Catenin Pathway

O-GlcNAcylation Modification in Immune Cells: Potential Therapeutic Implications of Sepsis

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