Glutamine Blocks Interleukin-13-Induced Intestinal Epithelial Barrier Dysfunction

Li, Min; Oshima, Tadayuki; Ito, Chiyomi; Yamada, Mayumi; Tomita, Toshihiko; Fukui, Hirokazu; Miwa, Hiroto

doi:10.1159/000502953

Cited by 25 publications

(15 citation statements)

References 50 publications

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“…It is reported that addition of glutamine increased the expression of ZO-1 and occludin in methotrexate-treated Caco-2 cells (Beutheu et al, 2013). Glutamine increased the claudin-1 expression to reduce IL-13-induced barrier dysfunction (Li et al, 2019). In stressed humans, glutamine is known to regulate the expression of tight junction proteins and cellular localization in Caco-2 cell monolayers to maintain the intestinal barrier function (Li et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

Jin

Wang

et al. 2020

Front. Pharmacol.

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Corneal endothelial disease is a global sight-threatening disease, and corneal transplantation using donor corneas remains the sole therapeutic option. A previous work demonstrated that N (2)-alanyl-glutamine (Ala-Gln) protected against apoptosis and cellular stress, and maintained intestinal tissue integrity. In this pursuit, the present study aimed to examine the effect of Ala-Gln in the protection of the corneal endothelium and expand its range of potential clinical applications. Mice in the control group were intracamerally irrigated with Ringers lactate injection, whereas those in the experimental group were irrigated with Ringers lactate injection containing Ala-Gln. The mean intraocular pressure increased to 44 ± 3.5 mm Hg during intracameral irrigation (normal range 10.2 ± 0.4 mmHg). In vivo confocal microscopy results showed that the addition of Ala-Gln protected the morphology, structure, and density of the corneal endothelial cells. Optical Coherence Tomography (OCT) measurements showed that corneal thickness was not significantly different between the two groups, because of the immediate corneal edema after irrigation, but the addition of Ala-Gln obviously promoted the recovery of the corneal edema. Scanning electron microscopy indicated that the corneal endothelial cells were severely ruptured and exfoliated in the Ringer's group accompanied with cellular edema, when compared with the Ala-Gln group. The intracameral irrigation using Ala-Gln protected the structure and expression of cytoskeleton and Na-K-ATPase, which exhibited a regular distribution and significantly increased expression in comparison to Ringer's group. Furthermore, Ala-Gln maintained the mitochondrial morphology and increased the activity of mitochondria. Moreover, transmission electron microscopy showed that intracameral irrigation of Ala-Gln reversed the ultrastructural changes induced by the acute ocular hypertension in mice. Our study demonstrates that the intracameral irrigation of Ala-Gln effectively maintained the corneal endothelial pump function and barrier function by protecting the mitochondrial function and preventing the rearrangement of cytoskeleton in acute ocular hypertension in mice.

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Section: Discussionmentioning

confidence: 99%

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

Jin

Wang

et al. 2020

Front. Pharmacol.

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“…This improvement is associated with the restoration of the altered distribution of occludin and ZO-1 [69]. A recent study shows that pre-treatment with glutamine in vitro blocks the interleukin-13-induced intestinal epithelial barrier dysfunction characterized by a decrease of TEER and an increase of 4 KDa dextran permeability [70]. Glutamine deprivation not only affects the expression of tight junction proteins, but also reversely suppresses epithelial cell proliferation as shown in intestinal organoid cell culture [71].…”

Section: Glutaminementioning

confidence: 93%

Intestinal Permeability, Inflammation and the Role of Nutrients

et al. 2020

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The interaction between host and external environment mainly occurs in the gastrointestinal tract, where the mucosal barrier has a critical role in many physiologic functions ranging from digestion, absorption, and metabolism. This barrier allows the passage and absorption of nutrients, but at the same time, it must regulate the contact between luminal antigens and the immune system, confining undesirable products to the lumen. Diet is an important regulator of the mucosal barrier, and the cross-talk among dietary factors, the immune system, and microbiota is crucial for the modulation of intestinal permeability and for the maintenance of gastrointestinal tract (GI) homeostasis. In the present review, we will discuss the role of a number of dietary nutrients that have been proposed as regulators of inflammation and epithelial barrier function. We will also consider the metabolic function of the microbiota, which is capable of elaborating the diverse nutrients and synthesizing products of great interest. Better knowledge of the influence of dietary nutrients on inflammation and barrier function can be important for the future development of new therapeutic approaches for patients with mucosal barrier dysfunction, a critical factor in the pathogenesis of many GI and non-GI diseases.

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“…Gln regulated TJ integrity and distribution through calcium/calmodulin-dependent kinase 2 (CaMKK2)-AMP-activated protein kinase signaling in porcine epithelial cells (111). Recently, Gln alleviated IL-13-induced barrier dysfunction by increasing CLDN-1 expression, via disruption of the phosphatidylinositol-3-kinase/Akt signaling pathway (112).…”

Section: Amino Acidsmentioning

confidence: 99%

Present and Future Therapeutic Approaches to Barrier Dysfunction

et al. 2021

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There is converging and increasing evidence, but also uncertainty, for the role of abnormal intestinal epithelial barrier function in the origin and development of a growing number of human gastrointestinal and extraintestinal inflammatory disorders, and their related complaints. Despite a vast literature addressing factors and mechanisms underlying changes in intestinal permeability in humans, and its connection to the appearance and severity of clinical symptoms, the ultimate link remains to be established in many cases. Accordingly, there are no directives or clinical guidelines related to the therapeutic management of intestinal permeability disorders that allow health professionals involved in the management of these patients to carry out a consensus treatment based on clinical evidence. Instead, there are multiple pseudoscientific approaches and commercial propaganda scattered on the internet that confuse those affected and health professionals and that often lack scientific rigor. Therefore, in this review we aim to shed light on the different therapeutic options, which include, among others, dietary management, nutraceuticals and medical devices, microbiota and drugs, and epigenetic and exosomes-manipulation, through an objective evaluation of the scientific publications in this field. Advances in the knowledge and management of intestinal permeability will sure enable better options of dealing with this group of common disorders to enhance quality of life of those affected.

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Glutamine Blocks Interleukin-13-Induced Intestinal Epithelial Barrier Dysfunction

Cited by 25 publications

References 50 publications

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

Intestinal Permeability, Inflammation and the Role of Nutrients

Present and Future Therapeutic Approaches to Barrier Dysfunction

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