Glutamic Oxalacetic Transaminase (Colorimetric)

Sax, Sylvan M.; Moore, John J.; Nino, Hipolito V.; Edwards, David J.; Annino, Joseph S.

doi:10.1016/b978-0-12-609106-9.50020-8

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Root length and root weight were measured by a standard ruler and 1/1000 balance; if 60% of the length of the root appeared blackish or brownish, it was calculated as a black root, and the rest were calculated as white roots; the roots shorter than 1 cm in length or decomposed were not calculated in root length and root weight. Root bleeding was collected by the cotton traps method [16], SS was performed according to Hu et al [17], GOT activity was measured through the method of Sax et al [18], and GPT and GS activity was tested via the methods of Zhong et al [19] and Yu and Zhang [20], respectively.…”

Section: Samples and Measurementmentioning

confidence: 99%

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

et al. 2019

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Incorporating oilseed-rape straw in soil is one of the effective methods for enhancing the use efficiency of agricultural resources in the rape-rice rotation system. However, the impacts of oilseed-rape straw incorporation on root growth and dynamic changes in soil are still unclear. In order to provide a deeper understanding of the oilseed rape straw return on rice growth and productivity, the experiment was conducted in the field and in a specially-designed pots system from 2016 to 2017 by means of two straw returning methods and four straw returning amounts. In the early stage of rice growth (0–36 days after rice transplanting) the straw returning treatments decreased 1.0–8.6 mg/plant in bleeding density and 0.10–6.11, 0.06–0.31, and 0.52–0.84 μmol/(g h) in the activity of glutamine synthetase (GS), glutamic-oxalacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT), respectively. Oilseed rape straw returned by mulching induced negative impacts on new germinating roots in 0–10 cm of soil, while the negative impacts were observed on roots in 10–30 cm of soil for straw returned by plowing. In the later stage of rice growth (56–75 days after rice transplanting), oilseed rape straw returning produced some positive impacts on rice roots, which enhanced the yield of rice. Conclusively, our findings suggested that dynamic root growth and the activity of root enzymes are two major factors behind the slow reviving of rice after transplanting in the straw returning field. Plowing is a more appropriate method of straw returning than mulching in the rape-rice rotation system in the Sichuan basin area, with a straw incorporation rate of 3.0 t/hm2.

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Section: Samples and Measurementmentioning

confidence: 99%

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

et al. 2019

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“…colorimetric (13,14), flow injection analysis (15), amperometric (16), and spectrophotometric methods in which GOT is coupled with malate dehydrogenase (1 7) or citrate synthase (1 8).…”

Section: Introductionmentioning

confidence: 99%

Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained fromStreptomyces aureofaciens

1995

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A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Strepfomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation. 2305

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Tissue-Derived Plasma Enzymes

Fishman¹,

Doellgast²

1975

The Plasma Proteins

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Glutamic Oxalacetic Transaminase (Colorimetric)

Cited by 4 publications

References 26 publications

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained fromStreptomyces aureofaciens

Tissue-Derived Plasma Enzymes

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