Glutamatergic and Purinergic Receptor-Mediated Calcium Transients in Bergmann Glial Cells

Piet, Richard; Jahr, Craig E.

doi:10.1523/jneurosci.0462-07.2007

Cited by 82 publications

(104 citation statements)

References 50 publications

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“…Such a feedback step could account for why the apparent diffusion coefficient for spontaneous waves was somewhat slower than the estimated diffusion constant of ATP (200-400 m 2 /s) (35), raising the possibility of intermediate cell-to-cell signaling steps in wave propagation. Another source is molecular layer interneurons, which can release ATP after parallel fiber stimulation (19).…”

Section: Discussionmentioning

confidence: 99%

“…Activation of molecular layer interneurons can cause vasodilation (42). With ATP release from these cells (19) triggered transglial calcium waves could result in the calcium-dependent release of cyclooxygenases necessary for the local control of blood flow to metabolically active regions. Interestingly, stimulation of the climbing fiber pathway causes lactate accumulation originating from BG, an energy source for metabolically active neurons (43).…”

Section: Discussionmentioning

confidence: 99%

“…BG express a variety of G protein-coupled receptors that couple to calcium release from intracellular stores (17, 31), including P2Y receptors, which are activated by ATP (16,18,19). To test whether spontaneous transglial waves rely on a purinergic receptor-dependent mechanism, we imaged transglial waves in rats before, during, and after surface application of the P2 receptor antagonists PPADS (500 M, Fig.…”

Section: Spontaneous Transglial Calcium Waves Are Reversibly Blocked mentioning

confidence: 99%

“…4E), consistent with a P2 receptor-dependent mechanism. P2 receptor activation triggers calcium release from internal stores (19,32), which after activation can be refractory for tens of seconds as refilling occurs (33). We delivered 5 successive ATP injections at different-spaced time intervals (Fig.…”

Section: Spontaneous Transglial Calcium Waves Are Reversibly Blocked mentioning

confidence: 99%

“…We used in vivo 2-photon microscopy in combination with either bolus loading of calcium indicators or expression of the genetically encoded calcium sensor protein G-CaMP2 targeted to Bergmann glia (BG), the astrocytes of the molecular layer (ML). In brain slices, BG mobilize calcium in response to a number of neurotransmitters (16,17) and can generate subcellular domains of calcium release in response to synaptic stimulation via activation of purinergic receptors (18,19).…”

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confidence: 99%

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Radially expanding transglial calcium waves in the intact cerebellum

Hoogland

Kühn

Göbel

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

148

133

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Multicellular glial calcium waves may locally regulate neural activity or brain energetics. Here, we report a diffusion-driven astrocytic signal in the normal, intact brain that spans many astrocytic processes in a confined volume without fully encompassing any one cell. By using 2-photon microscopy in rodent cerebellar cortex labeled with fluorescent indicator dyes or the calcium-sensor protein G-CaMP2, we discovered spontaneous calcium waves that filled approximately ellipsoidal domains of Bergmann glia processes. Waves spread in 3 dimensions at a speed of 4 -11 m/s to a diameter of Ϸ50 m, slowed during expansion, and were reversibly blocked by P2 receptor antagonists. Consistent with the hypothesis that ATP acts as a diffusible trigger of calcium release waves, local ejection of ATP triggered P2 receptor-mediated waves that were refractory to repeated activation. Transglial waves represent a means for purinergic signals to act with local specificity to modulate activity or energetics in local neural circuits.astrocytes ͉ Bergmann glia ͉ in vivo ͉ 2-photon microscopy ͉ G-CaMP2

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Spontaneous Transglial Calcium Waves Are Reversibly Blocked mentioning

confidence: 99%

Section: Spontaneous Transglial Calcium Waves Are Reversibly Blocked mentioning

confidence: 99%

mentioning

confidence: 99%

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Radially expanding transglial calcium waves in the intact cerebellum

Hoogland

Kühn

Göbel

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

148

133

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Near‐field dosimetry for in vitro exposure of human cells at 60 GHz

Zhadobov

Sauleau

Augustine

et al. 2011

Bioelectromagnetics

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Due to the expected mass deployment of millimeter-wave wireless technologies, thresholds of potential millimeter-wave-induced biological and health effects should be carefully assessed. The main purpose of this study is to propose, optimize, and characterize a near-field exposure configuration allowing illumination of cells in vitro at 60 GHz with power densities up to several tens of mW/cm(2) . Positioning of a tissue culture plate containing cells has been optimized in the near-field of a standard horn antenna operating at 60 GHz. The optimal position corresponds to the maximal mean-to-peak specific absorption rate (SAR) ratio over the cell monolayer, allowing the achievement of power densities up to 50 mW/cm(2) at least. Three complementary parameters have been determined and analyzed for the exposed cells, namely the power density, SAR, and temperature dynamics. The incident power density and SAR have been computed using the finite-difference time-domain (FDTD) method. The temperature dynamics at different locations inside the culture medium are measured and analyzed for various power densities. Local SAR, determined based on the initial rate of temperature rise, is in a good agreement with the computed SAR (maximal difference of 5%). For the optimized exposure setup configuration, 73% of cells are located within the ±3 dB region with respect to the average SAR. It is shown that under the considered exposure conditions, the maximal power density, local SAR, and temperature increments equal 57 mW/cm(2) , 1.4 kW/kg, and 6 °C, respectively, for the radiated power of 425 mW.

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Sodium signals in cerebellar Purkinje neurons and Bergmann glial cells evoked by glutamatergic synaptic transmission

Bennay¹,

Langer²,

Meier³

et al. 2008

Glia

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Glial cells express specific high-affinity transporters for glutamate that play a central role in glutamate clearance at excitatory synapses in the brain. These transporters are electrogenic and are mainly energized by the electrochemical gradient for sodium. In the present study, we combined somatic whole-cell patch-clamp recordings with quantitative Na+ imaging in fine cellular branches of cerebellar Bergmann glial cells and in dendrites of Purkinje neurons to analyze intracellular Na+ signals close to activated synapses. We demonstrate that pressure application of glutamate and glutamate agonists causes local Na+ signals in the mM range. Furthermore, we analyzed the pharmacological profile, as well as the time course and spatial distribution of Na+ signals following short synaptic burst stimulation of parallel or climbing fibers. While parallel fibers stimulation resulted in local sodium transients that were largest in processes close to the stimulation pipette, climbing fibers stimulation elicited global sodium transients throughout the entire cell. Glial sodium signals amounted to several mM, were mainly caused by sodium influx following inward transport of glutamate and persisted for tens of seconds. Sodium transients in dendrites of Purkinje neurons, in contrast, were mainly caused by activation of AMPA receptors and had much faster kinetics. By reducing the driving force for sodium-dependent glutamate uptake, intracellular sodium accumulation in glial cells upon repetitive activity might provide a negative feedback mechanism, promoting the diffusion of glutamate and the activation of extrasynaptic glutamate receptors at active synapses in the cerebellum.

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Glutamatergic and Purinergic Receptor-Mediated Calcium Transients in Bergmann Glial Cells

Cited by 82 publications

References 50 publications

Radially expanding transglial calcium waves in the intact cerebellum

Radially expanding transglial calcium waves in the intact cerebellum

Near‐field dosimetry for in vitro exposure of human cells at 60 GHz

Sodium signals in cerebellar Purkinje neurons and Bergmann glial cells evoked by glutamatergic synaptic transmission

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