Glutamate related osmoregulation of guanine nucleotide-binding protein G (I) α2 from giant freshwater prawn (Macrobrachium rosenbergii) during molting and salinity stress

Lu, Zhijie; Guang, Yang; Qin, Zhendong; Shen, Han; Zhang, Menglan; Shi, Fei; Li, Jun; Babu, V. Sarath; Lin, Li

doi:10.1016/j.aquaculture.2020.735000

Cited by 15 publications

(8 citation statements)

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“…The amplified cDNA fragments were then cloned by insertion into a pMD18-T vector, followed by sequencing with M13 primers in forward or reverse. After verification of the final sequences, they were exposed to the cluster analysis in NCBI [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

“…The conditions provided were 95 °C for 3 min, 40 cycles at 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 20 s, and finally 4 °C for 5 min. The ratio of expression in correlation of the target genes was standardized with an internal reference β-actin gene [ 42 , 43 ], and the 2 −ΔΔCT method was used to calculate expression levels of Mr - IAGBP and Mr - IAG [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

“…An immunohistochemistry (IHC) assay method was modified for this study and then applied [ 42 , 43 , 48 ]. In short, various tissues (heart, testis, eyestalk, nerve cord, muscle, androgenic gland, hepatopancreas, and brain) were set at 4 °C for 24 h in 4% paraformaldehyde in paraffin.…”

Section: Methodsmentioning

confidence: 99%

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Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (Macrobrachium rosenbergii)

Guang

Qin

et al. 2020

IJMS

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Giant freshwater prawns (Macrobrachium rosenbergii) are commonly found throughout the world. The size of the male giant freshwater prawn is much larger than that of the female. Therefore, understanding the molecular mechanism that underlies the sexual differentiation of M. rosenbergii is of both commercial and scientific importance. Insulin-like androgenic gland hormone (IAG) plays a key role in the differentiation of sex in M. rosenbergii. Although IAG has been investigated, the regulatory relationship between IAG and its binding protein partner, the insulin-like androgenic gland hormone-binding protein (IAGBP), has not been studied in M. rosenbergii. Here, we cloned and characterized the IAGBP from M. rosenbergii (Mr-IAGBP) for the very first time. Transcriptomic analysis showed that Mr-IAGBP mRNA was detected in a wide array of tissues with the highest expression found in the androgenic gland. The importance of IAG in male development was further demonstrated by an increase in IAG transcripts during the development of the androgenic gland and Mr-IAG was only highly transcribed in the androgenic gland of M. rosenbergii. Interestingly, we found that the Mr-IAG gene expression started during the 20th-day larva after hatching stage (LH20), followed (20th-day post-larval stage, PL20) by a gradual elevation of Mr-IAGBP levels. The levels of both genes peaked at the adult stage. The relationship between Mr-IAGBP and Mr-IAG was further analyzed using RNA interference. The injection of Mr-IAGBP double-stranded RNA (dsRNA) significantly reduced the transcription of Mr-IAG, while the amount of Mr-IAGBP mRNA and the translation of IAGBP protein was significantly reduced by the injection of Mr-IAG dsRNA. These results revealed that IAGBP is involved in IAG signaling. Furthermore, our data supports the hypothesis that (IAG and IAGBP)-IAG receptor signaling schemes exist in M. rosenbergii. Our results will provide important information for the further study of determining the sex of M. rosenbergii.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (Macrobrachium rosenbergii)

Guang

Qin

et al. 2020

IJMS

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“…Recalculation of the feeding rate was carried out weekly. The dry weight was determined using the principle of the constant mass method [11,12]. During the experiment, daily monitoring of feed intake and removal of feed residues before the next feeding were carried out.…”

Section: Water Quality Indicatorsmentioning

confidence: 99%

Study of the growth and survival parameters of Macrobrachium rosenbergii shrimp depending on the type of feed used

et al. 2021

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The aim of the study is to determine the effect of the feed type (Daphnia pulex-based feed and Dajana nano gran brand) on the water quality (presence of microorganisms and suspended solids) and on the growth and survival of Macrobrachium rosenbergii shrimp larvae. The study implementation plan involved 4 groups of shrimps placed under the same conditions, which were evaluated for 14 weeks through periodic observations and biometrics. The data obtained recorded significant differences between the groups where the best growth and survival results were obtained in organisms that received Dajana nano gran feed, regardless of water quality. In addition, the values of protein in shrimp meat up to 45% and different amounts of fatty acids were obtained. It was determined that the Daphnia pulex-based feed showed lower results in the growth rate and survival rate of Macrobrachium rosenbergii shrimp.

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“…The reverse transcription reaction was in a total volume of 20 μl, which contains 10 μl AceQ® qPCR SYBR® Green Master Mix (Vazyme, Nanjing, China), 1 μl cDNA, 7 μl DEPC treated water, and 1 μl of each particular primer (list in Table 1). The quantitative real-time PCR cycling conditions were as stated: 95 °C for 3 minutes, further at 95 °C for 15 seconds of 40 cycles, for 30 seconds at 60 °C, for 20 seconds at 72 °C, and nally for 5 minutes at 4 °C [28]. All reactions of each gene in each sample were carried out in triplicate.…”

Section: Hydrophila Infection and Immune Genes Expressionmentioning

confidence: 99%

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

Yang

Zhang

et al. 2020

Preprint

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Background: Fish blood contains RBCs (Red blood cells) as the major cells. Unlike the absence of nucleus in mammalian RBC, fish RBC contains a nucleus. Previous researches have indicated that fish RBCs have a significant function in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost RBCs remains enigmatic. To decipher the mechanisms, after the infection of A. hydrophila, transcriptomic profiling of grass carp RBCs was analyzed. Results: Outcomes demonstrated that there were 2144 altogether DEGs (Differently Expressed Genes) between the A. hydrophila non-disease groups, which includes 817 up-regulated and 1327 down-regulated DEGs. Differently Expressed Genes were allocated to 45 GO terms, including 20 natural procedure terms, 14 cell related element, and 11 terms related to molecular functions. Likewise, the expression levels of cytokines by Quantitative Reverse Transcription Polymerase Chain Reaction showed that they (e.g. CCL4, CCL11, CCL20, IL-4, IL-12, and IFNα) were significantly increased after A. hydrophila infection, which was identical to the expression patterns of transcriptomic data. The infection could cause the apoptosis of RBCs which was confirmed by annexin V/PI assay. To further elucidate the apoptosis pathway, the expression levels of genes by mRNA included in cellular apoptosis were monitored. The outcomes indicated that the expressions of mRNA of p53, Fas, Leucine-rich repeats, death domain-containing (LRDD) and caspase 8 were all significantly increased, respectively. Conclusions: Our results support the notion that A. hydrophila infection could activate Fas-related death receptor apoptosis pathway in grass carp RBCs, which will reveal another insight on the mechanisms underlying the antibacterial immunity of teleost fish RBCs.

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Glutamate related osmoregulation of guanine nucleotide-binding protein G (I) α2 from giant freshwater prawn (Macrobrachium rosenbergii) during molting and salinity stress

Cited by 15 publications

References 61 publications

Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (Macrobrachium rosenbergii)

Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (Macrobrachium rosenbergii)

Study of the growth and survival parameters of Macrobrachium rosenbergii shrimp depending on the type of feed used

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

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