Glutamate Metabolism in a Glutamate-producing Bacterium,Brevibacterium flavum

Amino Acid Aminotransferases in an Amino Acid-producing Bacterium,Brevibacterium flavum

“…2247 (ATCC 14067), its aspartate auxotroph No. 102-7,2) and prototrophic revertants 3 ) from strain No. 102-7, No.…”

“…102-7-95,-71, -295, -87, -125, -126, -203, and -172 were used. Medium 32 was the same as medium 29 3 ) except that 25 g of monosodium glutamate, 20 g of glucose and 7.5 g of (NH4)2S04 were used instead of 50 g of monosodium glutamate. Medium-7, -15, and -19 were the same as described in previous papers.…”

“…In our previous studies, aminotransferase(s) involved in the reaction between aspartate and glutamate has been confirmed to be essential for aspartate biosynthesis 2 ) and for the utilization of glutamate as carbon and nitrogen sources 3 ) in this bacterium. Partial purification and some of the properties of another aminotransferase of this bacterium,which catalyzes formation of pretyrosine, a precursor of tyrosine, have also been reported previously.…”

Glutamate Transport and Production inBrevibacterium flavum

“…2247, and mutants unable to grow on glutamate, Nos. 741, 751, 753, 106 and 727 1 ) derived from No. 2247 and 112 1 ) derived from 1646, were used.…”

“…2247 and 112 1 ) derived from 1646, were used. Media 7,6) 20,9) 22,9>, 29 1 ) and 32 10 ) were described previously.…”