Glutamate imaging (GluCEST) lateralizes epileptic foci in nonlesional temporal lobe epilepsy

Davis, Kathryn A.; Nanga, Ravi Prakash Reddy; Das, Sandhitsu R.; Chen, Stephanie H.; Hadar, Peter N.; Pollard, John R.; Lucas, Timothy H.; Shinohara, Russell T.; Litt, Brian; Hariharan, Hari; Elliott, Mark A.; Detre, John A.; Reddy, Ravinder

doi:10.1126/scitranslmed.aaa7095

Cited by 165 publications

(199 citation statements)

References 55 publications

Supporting

Mentioning

191

Contrasting

Unclassified

Order By: Relevance

“…A gluCEST MRI brain study correctly lateralized the temporal lobe seizure focus in patients with epilepsy, which was corroborated with MR spectroscopy (Fig. ) . Relative glutamate concentrations in the spinal cord may serve as an additional biomarker for multiple sclerosis, which may complement the mobile protein content measurements made with APT MRI …”

Section: Glucest Mri Of Neurological Disorderssupporting

confidence: 58%

“…As another advantage, glutamate is often present at higher concentrations than other metabolites in the brain, which facilitates the CEST detection of glutamate. The imaging of glutamate with CEST MRI, known as gluCEST MRI, has been successfully performed in healthy brain tissue and the spinal cord . A 3.6 μT saturation power is used to generate strong CEST contrast, and this relatively high saturation power is reasonable considering the separation of the MR frequencies of glutamate and water, and the fast exchange rate of the labile protons on glutamate.…”

Section: Glucest Mri Of Neurological Disordersmentioning

confidence: 99%

See 1 more Smart Citation

Clinical applications of chemical exchange saturation transfer (CEST) MRI

Jones

Pollard

Pagel

2017

Magnetic Resonance Imaging

223

246

View full text Add to dashboard Cite

Chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) has been developed and employed in multiple clinical imaging research centers worldwide. Selective radiofrequency (RF) saturation pulses with standard 2D and 3D MRI acquisition schemes are now routinely performed, and CEST MRI can produce semiquantitative results using magnetization transfer ratio asymmetry (MTRasym) analysis while accounting for B0 inhomogeneity. Faster clinical CEST MRI acquisition methods and more quantitative acquisition and analysis routines are under development. Endogenous biomolecules with amide, amine, and hydroxyl groups have been detected during clinical CEST MRI studies, and exogenous CEST agents have also been administered to patients. These CEST MRI tools show promise for contributing to assessments of cerebral ischemia, neurological disorders, lymphedema, osteoarthritis, muscle physiology, and solid tumors. This review summarizes the salient features of clinical CEST MRI protocols and critically evaluates the utility of CEST MRI for these clinical imaging applications. Level of Evidence: 5 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;47:11–27.

show abstract

Section: Glucest Mri Of Neurological Disorderssupporting

confidence: 58%

Section: Glucest Mri Of Neurological Disordersmentioning

confidence: 99%

Clinical applications of chemical exchange saturation transfer (CEST) MRI

Jones

Pollard

Pagel

2017

Magnetic Resonance Imaging

223

246

View full text Add to dashboard Cite

show abstract

“…This method was also used in studying the Huntington's disease mouse model in which the decreased GluCEST was measured in the striatal region of the brain . This technique has also been demonstrated in a proof‐of‐principle study in human brain and was recently used in small studies to show differences in brain glutamate levels between patients compared with healthy controls …”

Section: Introductionmentioning

confidence: 99%

Reproducibility of 2DGluCEST in healthy human volunteers at 7 T

Nanga

DeBrosse

Kumar

et al. 2018

Magnetic Resonance in Med

Self Cite

View full text Add to dashboard Cite

PurposeTo investigate the reproducibility of gray and white matter glutamate contrast of a brain slice among a small group of healthy volunteers by using the 2D single‐slice glutamate CEST (GluCEST) imaging technique.MethodsSix healthy volunteers were scanned multiple times for within‐day and between‐day reproducibility. One more volunteer was scanned for within‐day reproducibility at 7T MRI. Glutamate CEST contrast measurements were calculated for within subjects and among the subjects and the coefficient of variations are reported.ResultsThe GluCEST measurements were highly reproducible in the gray and white matter area of the brain slice, whether it was within‐day or between‐day with a coefficient of variation of less than 5%.ConclusionThis preliminary study in a small group of healthy volunteers shows a high degree of reproducibility of GluCEST MRI in brain and holds promise for implementation in studying age‐dependent changes in the brain.

show abstract

“…In a recent study using GLU chemical exchange saturation transfer imaging in patients with temporal lobe epilepsy, increased glutamate was invariably found in the epileptic temporal lobe (Davis et al, 2015). In the present study, we utilized GLU 1 H-MRSI to test if children with unilateral SWS show increased GLU levels in the affected (epileptic) hemisphere as compared to the unaffected side.…”

Section: Introductionmentioning

confidence: 99%

Imaging increased glutamate in children with Sturge–Weber syndrome: Association with epilepsy severity

Juhász

Xuan

et al. 2016

Epilepsy Research

View full text Add to dashboard Cite

Background Sturge-Weber syndrome (SWS) is strongly associated with epilepsy. Brain tissue studies have suggested that epileptic activity in SWS is driven by glutamatergic synaptic activity. Here, we used proton magnetic resonance spectroscopic imaging (MRSI) to test if glutamate (GLU) concentrations are increased in the affected hemisphere and if such increases are associated with severity of epilepsy in children with SWS. We also studied the metabolic correlates of MRSI abnormalities, using glucose positron emission tomography (PET) imaging. Methods 3T MRI and glucose PET were performed in 10 children (age: 7–78 months) with unilateral SWS and a history of epilepsy. MRSI data were acquired from the affected (ipsilateral) and non-affected (contralateral) hemispheres. GLU, N-acetyl-aspartate (NAA) and creatine (Cr) were quantified in multiple voxels; GLU/Cr and NAA/Cr ratios were calculated and compared to seizure frequency as well as glucose PET findings. Results The highest GLU/Cr ratios were found in the affected hemisphere in all children except one with severe atrophy. The maximum ipsilateral/contralateral GLU/Cr ratios ranged between 1.0–2.5 (mean: 1.6). Mean ipsilateral/contralateral GLU/Cr ratios were highest in the youngest children and showed a strong positive correlation with clinical seizure frequency scores assessed at the time of the scan (r=0.88, p=0.001) and also at follow-up (up to 1 year, r=0.80, p=0.009). GLU increases in the affected hemisphere coincided with areas showing current or previous increases of glucose metabolism on PET in 5 children. NAA/Cr ratios showed no association with clinical seizure frequency. Conclusions Increased glutamate concentrations in the affected hemisphere, measured by MRSI, are common in young children with unilateral SWS and are associated with frequent seizures. The findings lend support to the role of excess glutamate in SWS-associated epilepsy.

show abstract

Glutamate imaging (GluCEST) lateralizes epileptic foci in nonlesional temporal lobe epilepsy

Cited by 165 publications

References 55 publications

Clinical applications of chemical exchange saturation transfer (CEST) MRI

Clinical applications of chemical exchange saturation transfer (CEST) MRI

Reproducibility of 2DGluCEST in healthy human volunteers at 7 T

Imaging increased glutamate in children with Sturge–Weber syndrome: Association with epilepsy severity

Contact Info

Product

Resources

About