Glutamate, GABA, glycine and taurine modulate serotonin synthesis and release in rostral and caudal rhombencephalic raphe cells in primary cultures

Becquet, Denis; Héry, Micheline; François‐Bellan, Anne‐Marie; Giraud, Pierre; Deprez, Paule; Faudon, M.; Fache, Marie‐Pierre; Héry, F.

doi:10.1016/0197-0186(93)90118-o

Cited by 43 publications

(17 citation statements)

References 48 publications

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“…It has been suggested that the failure of GABA itself to induce effects on cultured cells may be attributable to the presence of endogenous GABA (i.e. that released by GABA neurons; Barbin et al, 1993;Becquet et al, 1993;Liu et al, 1997). A large number of GABA-ir cells are usually present in cultures after proliferation and differentiation of neurospheres of mesencephalic precursors or stem cells (Arenas, 2002;Barberi et al, 2003), as also observed in the present study.…”

Section: Discussionsupporting

confidence: 57%

“…It is also possible that GABA receptor antagonists or GABA receptor activation induce significant changes in 5-HT release without significant changes in the number of serotonergic neurons. It has been observed that inhibition of GABA A receptors or activation of GABA B receptors decreases 5-HT release in primary cultures of raphe cells (Becquet et al, 1993), and this may explain the results observed in the present experiments after treatment of cultures with GABA A or GABA B receptor antagonists. However, we have recently observed that 5-HT 4 and 5-HT 7 receptors located in glial cells play a major role in the decreasing effect of serotonin on generation of DA neurons from neurospheres, which is inhibited by elimination of glial cells with Ara-C (Parga et al, 2006).…”

Section: Discussionsupporting

confidence: 48%

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Effects of GABA and GABA receptor inhibition on differentiation of mesencephalic precursors into dopaminergic neurons in vitro

Rodríguez‐Pallares

Guerra

Labandeira‐Garcia

2007

Developmental Neurobiology

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ABSTRACT:Neurotransmitters have been shown to control CNS neurogenesis, and GABA-mediated signaling is thought to be involved in the regulation of nearly all key developmental stages. Generation of dopaminergic (DA) neurons from stem/precursor cells for cell therapy in Parkinson's disease has become a major focus of research. However, the possible effects of GABA on generation of DA neurons from proliferating neurospheres of mesencephalic precursors have not been studied. In the present study, GABA A , and GABA B receptors were found to be located in DA cells. Treatment of cultures with GABA did not cause significant changes in generation of DA cells from precursors. However, treatment with the GABA A receptor antagonist bicuculline (10 À5 M) led to a significant increase in the number DA cells, and treatment with the GABA B receptor antagonist CGP 55845 (10 À5 M) to a significant decrease. Simultaneous treatment with bicuculline and CGP 55845 did not induce significant changes. Apoptotic cell death studies and bromodeoxyuridine immunohistochemistry indicated that the aforementioned differences in generation of DA neurons are not due to changes in survival or proliferation of DA cells, but rather to increased or decreased differentiation of mesencephalic precursors towards the DA phenotype. The results suggest that these effects are exerted via GABA receptors located on DA precursors, and are not an indirect consequence of effects on the serotonergic or glial cell population. Administration of GABA A receptor antagonists in the differentiation medium may help to obtain higher rates of DA neurons for potential use in cell therapy for Parkinson's disease. '

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Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 48%

Effects of GABA and GABA receptor inhibition on differentiation of mesencephalic precursors into dopaminergic neurons in vitro

Rodríguez‐Pallares

Guerra

Labandeira‐Garcia

2007

Developmental Neurobiology

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“…Interestingly, 5-HT neurons express both GAD (Belin et al, 1983;Nanopoulos et al, 1982), GABA-R1 receptor subunit (Abellan et al, 2000) and vesicular glutamate transporter type 3 (vGLUT3) (Stornetta et al, 2005), suggesting that some of the 5-HT neurons in rostral raphe cultures may produce GABA or glutamate. Consistent with this, amino acid neurotransmitters GABA, glycine, taurine and glutamate were reported in rostral raphe cultures from E15 rat embryos (12 DIV), and were found to regulate 5-HT metabolism (Becquet et al, 1993).…”

Section: Characterization Of Raphe Culturesmentioning

confidence: 74%

Characterization of rat rostral raphe primary cultures: Multiplex quantification of serotonergic markers

Czesak

Burns

Lenicov

et al. 2007

Journal of Neuroscience Methods

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“…Consequently, the stimulation of GABA A receptors reduces several neurochemical serotonergic indices in dorsal striatum (Nishikawa and Scatton, 1985;Becquet et al, 1990), a forebrain region innervated exclusively by serotonergic fibers of the DRN (Azmitia and Segal, 1978). Also, GABA inhibits 5-HT synthesis and its release from cultured serotonergic neurons (Becquet et al, 1993). Serotonergic neurons of the DRN of the rat are also inhibited by GABA B agonists, an effect involv-ing a voltage-dependent, pertussis toxin-sensitive, inwardly rectifying potassium channel coupled to a G protein (Innis and Aghajanian, 1987;Colmers and Williams, 1988;Innis et al, 1988;Williams et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

Abellán¹,

Jolas

Aghajanian

et al. 2000

Synapse

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We assessed the role of GABA(B) receptors in the control of serotonergic (5-HT) neurons of the dorsal raphe nucleus (DRN) by using microdialysis in vivo and intra- and extracellular recording in vitro in the rat. The GABA(B) agonist R(+)baclofen (but not the inactive S(-)enantiomer) enhanced the 5-HT output in the DRN (4. 7-fold at 15 mg/kg s.c.) and, to a much lesser extent, striatum of unanesthetized rats. Phaclofen (2 mg/kg s.c.) antagonized the effects of 6 mg/kg R(+)baclofen in dorsal striatum. Using dual-probe microdialysis, R(+)baclofen (0.1-100 microM) applied in the DRN enhanced the local 5-HT output (4.5-fold at 100 microM) but decreased that in striatum at 100 microM. At concentrations higher than 100 microM there was a moderate decrement in the elevation of 5-HT in the DRN. In midbrain slices, bath R(+)baclofen exerted a biphasic effect on DRN 5-HT neurons. Consistent with a reduced striatal 5-HT release when infused in the DRN, R(+)baclofen (0.1-30 microM) induced an outward current in 5-HT neurons (IC(50) = 1.4 microM). Lower R(+)baclofen concentrations (0.01-1 microM) preferentially reduced GABAergic inhibitory postsynaptic currents induced by N-methyl-D-aspartate (20 microM) in 5-HT neurons (IC(50) = 72 nM). Using extracellular recordings, R(+)baclofen (300 nM) enhanced the ability of NMDA to induce firing in a subpopulation of serotonergic neurons. These results are consistent with a preferential activation by a low concentration of R(+)baclofen of presynaptic GABA(B) receptors on GABAergic afferents that could disinhibit 5-HT neurons and increase 5-HT release.

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Glutamate, GABA, glycine and taurine modulate serotonin synthesis and release in rostral and caudal rhombencephalic raphe cells in primary cultures

Cited by 43 publications

References 48 publications

Effects of GABA and GABA receptor inhibition on differentiation of mesencephalic precursors into dopaminergic neurons in vitro

Effects of GABA and GABA receptor inhibition on differentiation of mesencephalic precursors into dopaminergic neurons in vitro

Characterization of rat rostral raphe primary cultures: Multiplex quantification of serotonergic markers

Dual control of dorsal raphe serotonergic neurons by GABAB receptors. Electrophysiological and microdialysis studies

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