GLUT1 Glucose Transporter in the Lactating Mammary Gland in the Rat.

Takata, Kuniaki; Fujikura, Keiko; Suzuki, Masakazu; Suzuki, Takeshi; Honda, Hiroshi

doi:10.1267/ahc.30.623

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…For the production of lactose, the mammary gland takes up a great amount of glucose using GLUT. GLUT1 is the only or the most predominant isoform of GLUTs expressed in the mammary gland and is localized to the basolateral membrane of acinar cells (Camps et al 1994;Takata et al 1997;Shennan and Peaker 2000), as conWrmed by the present study. The expression of MCT1 in the mammary gland is comparable to GLUT1 in intensity and subcellular localization.…”

Section: Expression Of Mct1 In the Mammary Glandssupporting

confidence: 88%

Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Takebe

Nio‐Kobayashi

Takahashi-Iwanaga

et al. 2008

Histochem Cell Biol

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Proton-coupled monocarboxylate transporters (MCTs) are essential for the transport of lactate, ketone bodies, and other monocarboxylates through the plasma membrane, but the direction and substrates of transporting in loco remain unclear. The present study examined the expression and subcellular localization of MCTs in lipogenic organs. An in situ hybridization survey of major MCT subtypes detected an intense expression of MCT1 mRNA in the mammary gland, Harderian gland, and sebaceous gland. The MCT1 immunoreactivity was found baso-laterally in acinar cells of the mammary and Harderian glands. Alveolar cells of sebaceous glands in the skin, eyelids, and penis contained the membrane-associated MCT1 immunoreactivity along the entire length of the cell surface at the margin of alveoli. These MCT1-expressing exocrine glands possessed more abundant transcripts of acetyl-CoA carboxylase-1, a key enzyme for lipogenesis, than did representative lipogenetic organs such as the liver. Since the secretions from these glands contain fat as a major product, the cellular localization of MCT1 suggests the involvement of the transporter in the uptake of lactate, acetate, and other monocarboxylates for production of medium- and long-chain fatty acids.

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Section: Expression Of Mct1 In the Mammary Glandssupporting

confidence: 88%

Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Takebe

Nio‐Kobayashi

Takahashi-Iwanaga

et al. 2008

Histochem Cell Biol

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“…3H-J). Further, we performed double labeling for AQP3 and the sugar transporter GLUT1 that is present in basolateral membranes of secretory epithelial cells (Takata et al 1997). AQP3 was colocalized with GLUT1, demonstrating that AQP3 was restricted to the basolateral membrane of secretory cells (Fig.…”

Section: Aquaporin-3mentioning

confidence: 88%

Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland

Matsuzaki

Machida

Tajika

et al. 2005

Histochem Cell Biol

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We examined the expression and immunolocalization of water-channel aquaporins in the mammary gland by reverse transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry. RT-PCR and immunoblotting revealed the expression of aquaporin-1 (AQP1) and AQP3 in the lactating rat mammary gland. AQP3 was detected in the alveolar epithelium and duct system whereas AQP1 was found in the capillaries and venules. AQP3 was present in the basolateral membrane of secretory epithelial cells and intralobular and interlobular duct epithelial cells. The main duct near the orifice in the nipple, which is comprised of a stratified epithelium, bore AQP3 in its basal and intermediate layers. AQP1 was located in both the apical and basolateral membranes of capillary and venule endothelia. AQP3 was not detected in virgin females. AQP3 was found in some differentiating mammary epithelial cells in the pregnant rat. AQP1 was present in capillaries and venules in the differentiating mammary gland of the pregnant rat and in the mammary fat pad of virgin females. We found a similar distribution of AQP1 and AQP3 in the mouse. AQP1 and AQP3 seem to play roles in the synthesis and/or secretion of milk.

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“…Previous immunoblottings of the rat sciatic nerves for GLUT1 have recognized a single band of 55 kDa (4) and of 55-60 kDa (22). Another immunoreactive band of GLUT1 existing at the lower position corresponded to the 43-kDa protein detected in the rat mammary gland by Takata et al (33).…”

Section: Expression Of Mct1 In the Peripheral Nerves Of Adultsmentioning

confidence: 88%

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

et al. 2008

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Peripheral nerves express GLUT1 in both endoneurial blood vessels and the perineurium and utilize glucose as a major energy substrate, as does the brain. However, under conditions of a reduced utilization of glucose, the brain is dependent upon monocarboxylates such as ketone bodies and lactate, being accompanied by an elevated expression of a monocarboxylate transporter (MCT1) in the blood-brain barrier. The present immunohistochemical study aimed to examine the expression of MCT1 in the peripheral nerves of mice. MCT1 immunoreactivity was found in the perineurial sheath and colocalized with GLUT1, while the endoneurial blood vessels expressed GLUT1 only. An intense expression of MCT1 in the perineurium was confirmed by Western blot and in situ hybridization analyses. Ultrastructurally, the MCT1 and GLUT1 immunoreactivities in the thick perineurium showed an intensity gradient decreasing towards the innermost layer. In neonates, the MCT1 immunoreactivity in the perineurium was intense, while the GLUT1 immunoreactivity was faint or absent. These findings suggest that peripheral nerves depend on monocarboxylates as a major energy source and that MCT1 in the perineurium is responsible for the supply of monocarboxylates to nerve fibers and Schwann cells.

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GLUT1 Glucose Transporter in the Lactating Mammary Gland in the Rat.

Cited by 12 publications

References 31 publications

Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

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