Glucosylceramide Biosynthesis is Involved in Golgi Morphology and Protein Secretion in Plant Cells

Melser, Su; Batailler, Brigitte; Peypelut, Martine; Poujol, Christel; Bellec, Yannick; Wattelet-Boyer, Valérie; Maneta-Peyret, Lilly; Faure, Jean-Denis; Moreau, Patrick

doi:10.1111/j.1600-0854.2009.01030.x

Cited by 55 publications

(52 citation statements)

References 45 publications

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“…In MDR1-MDCK cells, cell surface MDR1 colocalized with globotriaosyl ceramide (Gb 3 ), and a soluble analog of Gb 3 proved an MDR1inhibitor (De Rosa et al 2008). In plant cells, secretory products accumulate in swollen Golgi after GSL synthesis inhibition, and overexpression of such products results in a compensatory increase in GlcCer and sterol synthesis (Melser et al 2010). This is consistent with evidence that protein cargo sorting during Golgi transit is based on lipid gradients, rather than time-dependent cysternal transit (Patterson et al 2008).…”

Section: Gsls and Intracellular Protein Traffickingsupporting

confidence: 73%

Glycosphingolipid Functions

Lingwood¹

2011

Cold Spring Harbor Perspectives in Biology

105

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Section: Gsls and Intracellular Protein Traffickingsupporting

confidence: 73%

Glycosphingolipid Functions

Lingwood¹

2011

Cold Spring Harbor Perspectives in Biology

105

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“…Oil palm mesocarp (20 WAP) was subjected to cryofixation and cryosubstitution before sectioning. Slices of 50-80 nm thickness were observed by transmission electron microscope mostly as described (31).…”

Section: Methodsmentioning

confidence: 99%

Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning

Bourgis

Kilaru

Cao

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

334

406

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Oil palm can accumulate up to 90% oil in its mesocarp, the highest level observed in the plant kingdom. In contrast, the closely related date palm accumulates almost exclusively sugars. To gain insight into the mechanisms that lead to such an extreme difference in carbon partitioning, the transcriptome and metabolite content of oil palm and date palm were compared during mesocarp development. Compared with date palm, the high oil content in oil palm was associated with much higher transcript levels for all fatty acid synthesis enzymes, specific plastid transporters, and key enzymes of plastidial carbon metabolism, including phosphofructokinase, pyruvate kinase, and pyruvate dehydrogenase. Transcripts representing an ortholog of the WRI1 transcription factor were 57-fold higher in oil palm relative to date palm and displayed a temporal pattern similar to its target genes. Unexpectedly, despite more than a 100-fold difference in flux to lipids, most enzymes of triacylglycerol assembly were expressed at similar levels in oil palm and date palm. Similarly, transcript levels for all but one cytosolic enzyme of glycolysis were comparable in both species. Together, these data point to synthesis of fatty acids and supply of pyruvate in the plastid, rather than acyl assembly into triacylglycerol, as a major control over the storage of oil in the mesocarp of oil palm. In addition to greatly increasing molecular resources devoted to oil palm and date palm, the combination of temporal and comparative studies illustrates how deep sequencing can provide insights into gene expression patterns of two species that lack genome sequence information.

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“…Sphingolipids are essential components of the plasma membrane and nanoscale subcompartments of the plasma membrane, known as membrane rafts [1,2]. Some of these lipids are also found in the endomembrane system where they are involved in maintaining homeostasis through regulating cell secretion activity [3][4][5]. Beside their structural role, sphingolipids were further shown to participate in the control of autophagy and cell fate under stress conditions in animal and yeast cells [6,7].…”

Section: Introductionmentioning

confidence: 98%

Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS

et al. 2012

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In eukaryotic organisms, sphingolipids are major structural lipids of biological membranes and perform additional essential functions as signalling molecules. While long-chain bases (LCB), the common precursor to all sphingolipid classes, is represented by only one major molecular species in animals and fungi, up to nine LCB have been found in plants. In the absence of genuine plant sphingolipid references required for proper quantification, we have reinvestigated and optimized a protocol destined to the quantification of total plant LCB that relies on the use of gas chromatography-mass spectrometry (GC-MS). This rapid three-step protocol sequentially involves (1) the release of LCB from biological samples using barium hydroxide solution, (2) their oxidation into aldehydes by metaperiodate, and (3) the subsequent identification/quantification of these aldehydes by GC-MS. It is simple and reliable and enables separation of aldehydes upon their stero-specificity. It further enables the quantification of total LCB from a wide variety of samples including yeast and animal cell cultures.

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Glucosylceramide Biosynthesis is Involved in Golgi Morphology and Protein Secretion in Plant Cells

Cited by 55 publications

References 45 publications

Glycosphingolipid Functions

Glycosphingolipid Functions

Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning

Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS

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