Glucosylation of salicyl alcohol in cultured plant cells

Mizukami, Hajime; Terao, Toshimitsu; Miura, Hiroshi; Ohashi, Hiromu

doi:10.1016/s0031-9422(00)86961-7

Cited by 43 publications

(16 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…alcohol [5], salicylic acid [6], and HQ [7] [8]. Our Rauwolfia culture, optimized for indole alkaloid production, has excellent glucosylating activity for alkaloids, because it synthesizes under optimum conditions 1.6 g/1 medium of the glucoalkaloid raucaffricine from its aglycone vomilenine [9], and also glucosylates non-alkaloidal products.…”

mentioning

confidence: 99%

High‐Yield Formation of Arbutin from Hydroquinone by Cell‐Suspension Cultures of Rauwolfia serpentina

Lutterbach

Stöckigt

1992

Helvetica Chimica Acta

View full text Add to dashboard Cite

High-density cell-suspension cultures of Rauwolfia serpentina cultivated in a nutrition medium optimized for the production of the glucoalkaloid raucaffricine synthesize hydroquinone glycosides from continuously added hydroquinone with a total yield of23.87 g/1(18 g/l of arbutin and 5.87 g/l o f a hydroquinone diglycoside) in 7 days. This arbutin production is by far the highest formation of a natural product by plant-cell-culture systems reported to date.Introduction. -Plant cell-suspension cultures are an excellent tool for the investigation of the biosynthesis of natural products at the enzymatic level. Our recent studies with cultures of Rauwolfia serpentina (L.) BENTH, resulted in the isolation and identification of 33 different monoterpenoid indole alkaloids and 15 novel enzymes involved in their biosynthesis. These enzymes are highly substrate-specific proteins, including those catalyzing rather simple reactions, e.g. the glucosylation of the alkaloid vomilenine [ 13.To search this culture system for enzymes exhibiting more broad substrate acceptance, we conducted cell-free experiments and in vivo feeding experiments with various compounds. Feeding of e.g. the indole alkaloid ajmaline to cultivated Rauwolfia cells led to the detection of six novel alkaloids, named the raumacline group [2].In this paper, we describe, however, the high-yield in vivo biotransformation of hydroquinone (HQ)') into its p-D-glucoside (arbutin) and a hydroquinone diglycoside by the same culture system (Scheme).

show abstract

mentioning

confidence: 99%

High‐Yield Formation of Arbutin from Hydroquinone by Cell‐Suspension Cultures of Rauwolfia serpentina

Lutterbach

Stöckigt

1992

Helvetica Chimica Acta

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Production of natural products by plant cell biotechnology: results, problems and perspectives

Alfermann

Petersen

Fuss

2003

Plant Tissue Culture

View full text Add to dashboard Cite

show abstract

“…Several groups showed, that simple phenols like salicylic acid, salicyl alcohol or salicylaldehyde .can be glucosylated very effectively (Mizukami et al 1983(Mizukami et al , 1985(Mizukami et al , 1986(Mizukami et al , 1987Pilgrim 1970;Petersen et al 1992;Tabata et al 1976Tabata et al , 1988Tanaka et al 1990). Several groups showed, that simple phenols like salicylic acid, salicyl alcohol or salicylaldehyde .can be glucosylated very effectively (Mizukami et al 1983(Mizukami et al , 1985(Mizukami et al , 1986(Mizukami et al , 1987Pilgrim 1970;Petersen et al 1992;Tabata et al 1976Tabata et al , 1988Tanaka et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Plant Tissue Culture

Laimer¹,

Rücker²

2003

View full text Add to dashboard Cite

PrefaceOn February 6 th , 1902 appeared Gottlieb Haberlandts publication on "Culturversuche mit isolierten Pflanzenzellen" by the Imperial Academy of Sciences in Vienna, Austria. This paper contains Haberlandt's vision on the totipotency of plant cells, an idea with worldwide dimensions representing the actual beginning of tissue culture. When Haberlandt started culturing isolated plant cells in artificial nutrient media, he was mainly interested in cell to cell relationships within a complex multicellular organism. Haberlandt had studied the physiology of the plant tissues for a long time, and such profound dedication allowed him to understand the connection between physiology and anatomy. On that basis he has been able to make such a daring vision.In fact, it was only in 1912 that A Carrel succeeded in establishing the first animal cell cultures -which was awarded a Nobel prize -and in 1934 and 1939 respectively, the first plant tissue cultures were achieved by P.A White, R.J. Gautheret and P. Nobecourt. Commercial applications of plant tissue culture followed only in the seventies. We consider this an excellent example for the time frame required from a vision to its realisation and further to its development and broad range application.We take this anniversary also as an opportunity to remind all of us, that for developing ideas time is needed together with right conditions and atmosphere. Universities in the past used to provide, what now we are risking to lose with our hectic life style and short-term planning.This book pays homage to a great Austrian scientist, describing his life and his work and the further development, success and application of his idea. Most students and scientists relate the name Haberlandt with the first lectures of plant tissue culture. But only few are acquainted with the original pieces of work, which apart from their scientific interest are true artistic masterpieces. That is why the first part of the book contains a facsimile of the original paper and its first translation into English by AD. Krikorian and D.L. Berquam in 1969. Furthermore we find it worthwhile to pay some closer attention to the way experiments were planned, carried out and evaluated by Haberlandt in his time.It was also our intention to show the development which the initial idea has made in the past spreading into the various methods and fields of application. The second and third part of the book describe Haberlandt's life and work and historical aspects of the development of plant tissue culture in the beginnings. Who could give a better glance into the early days of plant tissue culture than R.J. Gautheret? It was from his laboratory in Paris that the senior editor brought this technique and knowledge to Vienna and established this field of Preface research here in Austria. The forth part of the book contains an overview of important topics of plant tissue culture representing the most promising areas of application at present time and giving an outlook into the future. The areas range from micropropagati...

show abstract

Glucosylation of salicyl alcohol in cultured plant cells

Cited by 43 publications

References 4 publications

High‐Yield Formation of Arbutin from Hydroquinone by Cell‐Suspension Cultures of Rauwolfia serpentina

High‐Yield Formation of Arbutin from Hydroquinone by Cell‐Suspension Cultures of Rauwolfia serpentina

Production of natural products by plant cell biotechnology: results, problems and perspectives

Plant Tissue Culture

Contact Info

Product

Resources

About