Glucosinolates, glycosidically bound volatiles and antimicrobial activity of Aurinia sinuata (Brassicaceae)

Blažević, Ivica; Radonić, Ani; Mastelić, Josip; Zekić, Marina; Skočibušić, Mirjana; Maravić, Ana

doi:10.1016/j.foodchem.2010.01.041

Cited by 52 publications

(35 citation statements)

References 26 publications

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“…The results found in above investigation were well correlated with the previous work [17][18][19][20]. The chemical composition revealed that isothiocyanates are the major components in both essential oils.…”

Section: Comparison Of Chemical Composition Antioxidant and Antimicrsupporting

confidence: 90%

Comparison of Chemical Composition, Antioxidant and Antimicrobial Potentials of Essential Oils and Oleoresins obtained from Seeds of Brassica Juncea and sinapisalba

Singh¹

2017

MOJFPT

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Recently there has been a renewed interest in improving health and fitness through the use of more natural products. Herbs and spices have been used for thousands of years to enhance the flavor, color and aroma of food, also known for their preservative and medicinal value. Recent reports suggest that cruciferous vegetables act as a good source of natural antioxidants due to the high levels of carotenoids, tocopherols and ascorbic acid. In addition to carotenoids, tocopherols, and ascorbic acid, most of the antioxidative effect related to plant food intake is mainly due to the presence of phenolic compounds, which have been associated with flavour and colour characteristics of fruits and vegetables. In this aspect, the popularity and consumption of vegetable Brassica species is increasing because of their nutritional value. Brassica foods are very nutritive, providing nutrients and healthpromoting Phytochemicals such as vitamins, carotenoids, fiber, soluble sugars, minerals, glucosinolates and phenolic compounds [1]. Brassica is one of the most ancient spices. It has 3 varieties namely black, brown and white/yellow. Brassica juncea (Brown mustard) is largely cultivated. Brown mustard plant produces tiny yellow colored flowers, which almost cover the plant. The plant is extensively grown for its mustard and as fodder crop. In addition, this species is known to be of great medicinal importance due to its antineoplastic, antimicrobial, and insecticidal activities. The plant is a folk remedy for arthritis, foot ache, lumbago, and rheumatism. Sinapis alba is an economically important plant of Brassicaceae, commonly known as yellow or white mustard, and growing well in hot and dry environments [2]. However, there is limited published research on essential oil composition and activity of both species in spite of the historical and traditional knowledge of both oils' medicinal importance. The popularity and consumption of vegetable Brassica species is increasing because of their nutritional value. Brassica crops have been related to the reduction of the risk of chronic diseases including cardiovascular diseases and cancer. Brassica foods are very nutritive, providing nutrients and health-promoting Phytochemicals such as vitamins, carotenoids, fiber, soluble sugars, minerals, glucosinolates and phenolic compounds [3]. Materials and MethodsBrown and yellow mustard seeds were purchased from local market of Gorakhpur. The fresh and mature berries of B.juncea and S.alba were washed; sun dried and pulverized into a fine AbstractMany consumers are demanding foods without what they perceive as artificial and harmful chemicals, including many used as antimicrobials and preservatives in food. Consequently, interest in more natural, antimicrobials as potential alternatives to conventional additives to extend shelf life and combat foodborne pathogens has heightened. In the present study, phytochemical, in vitro antioxidant and antimicrobial potentials of essential oils and oleoresins of Brassica juncea and Sinapis Alba seeds were ...

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Section: Comparison Of Chemical Composition Antioxidant and Antimicrsupporting

confidence: 90%

Comparison of Chemical Composition, Antioxidant and Antimicrobial Potentials of Essential Oils and Oleoresins obtained from Seeds of Brassica Juncea and sinapisalba

Singh¹

2017

MOJFPT

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“…The identification of GLs was previously reported in the genus Aurinia, i.e., in A. saxatilis and its subspecies A. saxatilis ssp. orientalis [3] and recently in A. sinuata [4]. The GLs identified were mostly sulfur-containing, viz., glucoalyssin (1), glucoiberin, glucoerucin, and glucoberteroin, branched aliphatic, viz., glucocochlearin, and olefinic GLs, viz., gluconapin (2) and glucobrassicanapin (3).…”

mentioning

confidence: 92%

Glucosinolate Profiling and Antimicrobial Screening of Aurinia leucadea (Brassicaceae)

Blažević

Radonić

Skočibušić

et al. 2011

Chemistry & Biodiversity

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Glucosinolates (GLs) were characterized in various aerial parts (stems, leaves, and flowers) of Aurinia leucadea (Guss.) C. Koch and quantified according to the ISO 9167-1 official method based on the HPLC analysis of desulfoglucosinolates. Eight GLs, i.e., glucoraphanin (GRA), glucoalyssin (GAL; 1), gluconapin (GNA; 2), glucocochlearin (GCC), glucobrassicanapin (GBN; 3), glucotropaeolin (GTL), glucoerucin (GER), and glucoberteroin (GBE) were identified. The total GL contents were 57.1, 37.8, and 81.3 μmol/g dry weight in the stems, leaves, and flowers, respectively. The major GL detected in all parts of the plant was 2, followed by 1 and 3. GC/MS Analysis of the volatile fractions extracted from the aerial parts of fresh plant material either by hydrodistillation or CH(2) Cl(2) extraction showed that these fractions mostly contained isothiocyanates (ITCs). The main ITCs were but-3-enyl- (55.6-71.8%), pent-4-enyl- (7.6-15.3%), and 5-(methylsulfinyl)pentyl ITC (0-9.5%), originating from the corresponding GLs 2, 3, and 1, respectively. The antimicrobial activity of the volatile samples was investigated by determining inhibition zones with the disk-diffusion method and minimal inhibitory concentrations (MIC) with the microdilution method. They were found to inhibit a wide range of bacteria and fungi, with MIC values of 2.0-32.0 μg/ml, indicating their promising antimicrobial potential, especially against the fungi Candida albicans and Rhizopus stolonifer as well as against the clinically important pathogen Pseudomonas aeruginosa.

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“…Individual peaks were identified by comparing their RI and mass spectra with those of authentic compounds by means of our homemade and the Wiley 275 spectral databases and with literature data [3] [27] [28]. The percentage composition of the samples was computed from the GC peak areas using the normalization method (without correction factors).…”

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confidence: 99%

Chemical Composition and Antimicrobial Activity of Volatiles from Degenia velebitica, a European Stenoendemic Plant of the Brassicaceae Family

Mastelić

Blažević

Kosalec

2010

Chemistry & Biodiversity

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Free and glucosidic bound leaf volatiles of Degenia velebitica were isolated and fractionated simultaneously into H(2)O-soluble, H(2)O-insoluble, and highly volatile compounds by hydrodistillation-adsorption (HDA) and analyzed by GC/MS. Among the 24 constituents identified, the main compounds obtained by the HDA method were S- and/or N-atom containing compounds, i.e., 6-(methylsulfanyl)hexanenitrile (10; 26.78%), dimethyl trisulfide (6; 26.35%), 3,4,5-trimethylpyrazole (17; 13.33%), hex-5-enenitrile (2; 10.11%), dimethyl tetrasulfide (8; 4.93%), and pent-4-enyl isothiocyanate (7; 4.45%). In addition, O-glycosidically bound volatiles and free volatiles were isolated by solvent extraction. Sixteen volatile O-aglycones and twelve free volatile components were identified. The main O-aglycones were eugenol (19; 24.15%), 2-methoxy-4-vinylphenol (11; 11.50%), and benzyl alcohol (20; 9.49%), and the main free volatiles were (9Z,12Z)-octa-9,12-dienic acid (38.35%), hexadecanoic acid (22.64%), and phytol (5.80%). The H(2)O-soluble volatile fraction obtained by HDA, containing mostly glucosinolate degradation products and 3,4,5-trimethylpyrazole (17), was evaluated for antimicrobial activity by determining inhibition zones with the diffusion method as well as minimal inhibitory concentrations (MIC) and minimal microbicidal concentrations (MMC) with the micro-dilution method. The fraction expressed activity against the tested Gram-positive and Gram-negative bacteria as well as against yeast, with MIC values equal to or lower than 16.7 μg/ml.

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Glucosinolates, glycosidically bound volatiles and antimicrobial activity of Aurinia sinuata (Brassicaceae)

Cited by 52 publications

References 26 publications

Comparison of Chemical Composition, Antioxidant and Antimicrobial Potentials of Essential Oils and Oleoresins obtained from Seeds of Brassica Juncea and sinapisalba

Comparison of Chemical Composition, Antioxidant and Antimicrobial Potentials of Essential Oils and Oleoresins obtained from Seeds of Brassica Juncea and sinapisalba

Glucosinolate Profiling and Antimicrobial Screening of Aurinia leucadea (Brassicaceae)

Chemical Composition and Antimicrobial Activity of Volatiles from Degenia velebitica, a European Stenoendemic Plant of the Brassicaceae Family

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