GLUCOSINOLATE CONTENT OF DEVELOPING RAPESEED (<i>Brassica napus</i> L. ’Midas’) SEEDLINGS

McGREGOR, D. I.

doi:10.4141/cjps88-048

Cited by 53 publications

(32 citation statements)

References 6 publications

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“…In agreement with results reported here, 4-hydroxyglucobrassicin has been found to be the major indol-3-ylmethylglucosinolate in broccoli, cauliflower and cabbage seeds, with varying concentrations depending upon the particular cultivar studied (McGregor, 1988;Sang et al, 1984;West et al, 2004). In the present study, the distribution of individual indol-3-ylmethylglucosinolates among particular tissues was found to follow a trend with glucobrassicin as the dominant indol-3-ylmethylglucosinolate in true leaves and neoglucobrassicin and 4-methoxyglucobrassicin in roots and hypocotyl, as reported elsewhere (McGregor, 1988;Sang et al, 1984).…”

Section: N Bellostas Et Al / Scientia Horticulturae XXX (2007) Xxx-supporting

confidence: 79%

“…In the present study, the distribution of individual indol-3-ylmethylglucosinolates among particular tissues was found to follow a trend with glucobrassicin as the dominant indol-3-ylmethylglucosinolate in true leaves and neoglucobrassicin and 4-methoxyglucobrassicin in roots and hypocotyl, as reported elsewhere (McGregor, 1988;Sang et al, 1984). Mature tissues tend to have higher concentrations of indol-3-ylmethylglucosinolates than young tissues (Brown et al, 2003;Fahey et al, 1997;Kushad et al, 1999), which have been related to de novo synthesis of this group of glucosinolates with growth (Chen and Andreasson, 2001;Clossais-Besnard and Larher, 1991;Kushad et al, 1999).…”

Section: N Bellostas Et Al / Scientia Horticulturae XXX (2007) Xxx-supporting

confidence: 59%

“…In agreement with our observations, Brown et al (2003) reported an increase in glucosinolate concentration during the first hours after seed imbibition in Arabidopsis thaliana, whereas later in the growth total glucosinolate concentration decreased. The decrease in glucosinolate concentration during seedling growth has also been reported in other brassicaceous species (ClossaisBesnard and Larher, 1991;McGregor, 1988;Pereira et al, 2002); it has been suggested to be a consequence of selective glucosinolate metabolism as well as dilution of glucosinolate concentration during tissue expansion (Chen and Andreasson, 2001). Active myrosinase expression seems to parallel the active catabolism that occurs in seedlings (James and Rossiter, 1991;Rask et al, 2000).…”

mentioning

confidence: 93%

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Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food

Bellostas

Kachlicki

Sørensen

et al. 2007

Scientia Horticulturae

116

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Consumption of plants of the species Brassica oleracea is related to a decreased incidence of certain cancer forms in humans, and this has been linked to the presence of glucosinolates in those vegetables. After ripe seeds, sprouts of some brassicaceous plants contain the highest concentration of these compounds and are therefore a good source of glucosinolates for chemoprotection. In the present experiments, the content and distribution of glucosinolates was determined in ripe seeds and sprouts (seedlings) of five varieties of B. oleracea (white cabbage, red cabbage, Savoy cabbage, broccoli and cauliflower) by high performance liquid chromatography. The type and concentration of individual glucosinolates varied according to variety of B. oleracea, plant parts in which they occurred and the sprouting period of the seed. Concentration of alkyl glucosinolates decreased whereas that of indol-3-ylmethylglucosinolates increased throughout the sprouting period. Roots had the highest glucosinolate concentration in four and seven day old sprouts whereas at both sprouting times, cotyledons had the highest concentration of alkylthio-and alkylsulphinylglucosinolates. #

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Section: N Bellostas Et Al / Scientia Horticulturae XXX (2007) Xxx-supporting

confidence: 79%

Section: N Bellostas Et Al / Scientia Horticulturae XXX (2007) Xxx-supporting

confidence: 59%

mentioning

confidence: 93%

See 1 more Smart Citation

Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food

Bellostas

Kachlicki

Sørensen

et al. 2007

Scientia Horticulturae

116

View full text Add to dashboard Cite

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“…It is important to understand the factors which determine the occurrence and levels of glucosinolates not only between species, but also between individual parts of the same plant. Ontogenic variation has also been reported in Brassica napus (Macfarlane Smith and Griffiths 1988 ;McGregor 1988 ;Clossais-Besnard and Larher 1991 ;Fieldsend and Milford 1994a,b) and B oleracea (Rosa et al 1996). Bergmann (1970) reported a decline in indole glucosinolates and 4-hydroxybenzylglucosinolate in developing seedlings of yellow mustard.…”

Section: Introductionmentioning

confidence: 89%

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Rosa

1997

J. Sci. Food Agric.

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Limited information is available on the glucosinolate variation within the Brassica plant and the relationship between the pattern and concentration of glucosinolates in the aerial parts and the roots has received little attention. Early studies carried out under Ðeld conditions have shown that glucosinolate levels may vary considerably throughout a 24 h period. The purpose of the present study was to show whether, under controlled conditions, temperature was a factor in glucosinolate variation and to determine whether such variation might be due to translocation of glucosinolates between the aerial parts of the plant and its roots. Cabbage seedlings were maintained at 20 and 30¡C over 2 days and leaves and roots sampled at 02 : 00 h, 06 : 00 h, 10 : 00 h, 14 : 00 h, 18 : 00 h and 22 : 00 h. The glucosinolates 2-propenyl-and 3-methylsulphinylpropyl-with an average of 261 and 167 kmol 100 g~1 DW, respectively, were the two main glucosinolates in the aerial part of the plant whilst in the roots 1-methoxyindol-3-ylmethyl-, 2-phenylethyl-and 3-methylsulphinylpropyl, with 495, 495 and 385 kmol 100 g~1 DW respectively, showed the highest average concentrations. Total and individual glucosinolates in the roots and in the aerial part of the plant showed the highest concentrations in the dark cycle, at 02 : 00 h and 22 : 00 h, respectively, whilst the lowest levels were during the light cycle, mainly at 18 : 00 h. The results suggest that temperature was not a major factor in the short-term variation in glucosinolate levels. Although there was a very high sig-niÐcant di †erence between the total glucosinolate levels in the aerial part of the plant (581 kmol 100 g~1 DW) and roots (2124 kmol 100 g~1 DW), the results of the present study do not support the concept of translocation between aerial part and roots, suggesting that other factors may be involved.

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“…200; Table III; Fig. 4) that is known to catalyze the hydrolysis of glucosinolates during germination (Chew, 1988;McGregor, 1988). Although intact glucosinolates are relatively nontoxic, their breakdown products have important biological effects, as for the goitrogenic species that perturb thyroid function or the very reactive isothiocyanates that present antibacterial and antifungal properties (Rask et al, 2000).…”

Section: Genes Responsive To Biotic and Abiotic Stresses Are Induced mentioning

confidence: 99%

Proteomic Analysis of Seed Dormancy in Arabidopsis

et al. 2006

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The mechanisms controlling seed dormancy in Arabidopsis (Arabidopsis thaliana) have been characterized by proteomics using the dormant (D) accession Cvi originating from the Cape Verde Islands. Comparative studies carried out with freshly harvested dormant and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins. The data suggested that proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release. Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination, which physiologically mimicked the behavior of D imbibed seeds. This application resulted in an alteration of the accumulation pattern of 71 proteins. There was a strong down-accumulation of a major part (90%) of these proteins, which were involved mainly in energetic and protein metabolisms. This feature suggested that exogenous ABA triggers proteolytic mechanisms in imbibed seeds. An analysis of de novo protein synthesis by two-dimensional gel electrophoresis in the presence of [ 35 S]-methionine disclosed that exogenous ABA does not impede protein biosynthesis during imbibition. Furthermore, imbibed D seeds proved competent for de novo protein synthesis, demonstrating that impediment of protein translation was not the cause of the observed block of seed germination. However, the two-dimensional protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA. Altogether, the data showed that the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium.

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GLUCOSINOLATE CONTENT OF DEVELOPING RAPESEED (Brassica napus L. ’Midas’) SEEDLINGS

Cited by 53 publications

References 6 publications

Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food

Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food

Daily Variation in Glucosinolate Concentrations in the Leaves and Roots of Cabbage Seedlings in Two Constant Temperature Regimes

Proteomic Analysis of Seed Dormancy in Arabidopsis

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