Glucosinolate Analysis in Whole Rapeseed by Near Infrared Reflectance Spectroscopy

Analysis of total glucosinolate content and individual glucosinolates in Brassica spp. by near‐infrared reflectance spectroscopy

“…NIRS calibrations for the analysis of total GSL content have been developed for Brassica napus and Brassica rapa (Renard et al 1987, Daun et al 1994, Brassica carinata (Velasco et al 1995), and Brassica juncea (A. De Haro, personal communication) because breeding for low GSL content has focused mainly on these species.…”

“…These methods were designed to estimate the total GSL content, and do not provide simultaneous information on the GSL profile (Sorensen 1985). The development of near-infrared reflectance spectroscopy (NIRS) calibrations to estimate the total GSL content in intact seeds represented a further advance, since it could be estimated in parallel with the analysis of other traits such as oil and protein content (Renard et al 1987).NIRS calibrations for the analysis of total GSL content have been developed for Brassica napus and Brassica rapa (Renard et al 1987, Daun et al 1994, Brassica carinata (Velasco et al 1995), and Brassica juncea (A. De Haro, personal communication) because breeding for low GSL content has focused mainly on these species.…”

“…These methods were designed to estimate the total GSL content, and do not provide simultaneous information on the GSL profile (Sorensen 1985). The development of near-infrared reflectance spectroscopy (NIRS) calibrations to estimate the total GSL content in intact seeds represented a further advance, since it could be estimated in parallel with the analysis of other traits such as oil and protein content (Renard et al 1987).…”

Simple and Rapid Method for the Selection of Individual Rapeseed Plants Low in Glucosinolates

“…The glucose release methods (Van Etten et al 1974, Smith andDacombe 1987) based on a colorimetric glucose oxidase/peroxidase assay including the clinical glucose test paper (Tes-Tape) method for urine sugar (Lein 1970, McGregor andDowney 1975), and the TRUBLUGLU meter method (Tholen et al 1993), have since been developed. Palladium method (Thies 1982, Møller et al 1985 and nondestructive quality evaluation methods using X-ray fluorescence spectroscopy (Schnug and Haneklaus 1988) and near-infrared reflectance spectroscopy (Renard et al 1987, Biston et al 1988 have also been developed. Specific ELISA assays have been developed for the determination of sinigrin and progoitrin that are major glucosinolates in Brussels sprouts (Van Doorn et al 1998).…”

Simultaneous Determination of Isothiocyanates, Indoles, and Oxazolidinethiones in Myrosinase Digests of Rapeseeds and Rapeseed Meal by HPLC

“…Methods that indicate the total amount of glucosinolates are often based on the measurement of released glucose (vanEtten et al, 1974;Thies, 1985;Fiebig and Kallweit, 1987) or bisulfate ions (Fiebig and Sendfeld, 1989;Sendfeld et al, 1988) by enzymatic assays. Other methods include the palladium test (Thies, 1982), the near-infrared method (Renard et al, 1987), and the X-ray fluorescence method Haneklaus, 1987, 1988;Haneklaus et al, 1994). Individual glucosinolates were determined directly by GLC analysis of the trimethylsilyl derivates of glucosinolates (Thies, 1976), HPLC analysis of the intact glucosinolates (Helboe et al, 1980), and HPLC analysis of desulfoglucosinolates (Minchinton et al, 1982;Spinks et al, 1984;Fiebig and Jo ¨rden, 1990) or indirectly by GLC analysis of the enzymatic hydrolysis products (Daxenbichler and vanEtten, 1977;vanEtten and Daxenbichler, 1977).…”