Glucose(xylose) isomerase production by Streptomyces sp. CH7 grown on agricultural residues

Chanitnun, Kankiya; Pinphanichakarn, Pairoh

doi:10.1590/s1517-83822012000300035

Cited by 19 publications

(6 citation statements)

References 27 publications

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“…The enzyme activity of XIs was inhibited by Ca 2+ . The inhibition with Ca 2+ was also reported by other workers ]. Thin layer chromatography revealed that considerable amount of D‐fructose was formed in 10 min during the isomerization reaction catalyzed by TnapXI.…”

Section: Discussionsupporting

confidence: 82%

Cloning, purification, and characterization of xylose isomerase from Thermotoga naphthophila RKU‐10

Fatima

Aftab

Haq

2016

J. Basic Microbiol.

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A 1.3 kb xyl-A gene encoding xylose isomerase from a hyperthermophilic eubacterium Thermotoga naphthophila RKU-10 (TnapXI) was cloned and over-expressed in Escherichia coli to produce the enzyme in mesophilic conditions that work at high temperature. The enzyme was concentrated by lyophilization and purified by heat treatment, fractional precipitation, and UNOsphere Q anion-exchange column chromatography to homogeneity level. The apparent molecular mass was estimated by SDS-PAGE to be 49.5 kDa. The active enzyme showed a clear zone on Native-PAGE when stained with 2, 3, 5-triphenyltetrazolium chloride. The optimum temperature and pH for D-glucose to D-fructose isomerization were 98 °C and 7.0, respectively. Xylose isomerase retains 85% of its activity at 50 °C (t1/2 1732 min) for 4 h and 32.5% at 90 °C (t1/2 58 min) for 2 h. It retains 90-95% of its activity at pH 6.5-7.5 for 30 min. The enzyme was highly activated (350%) with the addition of 0.5 mM Co(2+) and to a lesser extent about 180 and 80% with the addition of 5 and 10 mM Mn(2+) and Mg(2+) , respectively but it was inhibited (54-90%) in the presence of 0.5-10 mM Ca(2+) with respect to apo-enzyme. D-glucose isomerization product was also analyzed by Thin Layer Chromatography (Rf 0.65). The enzyme was very stable at neutral pH and sufficiently high temperature and required only a trace amount of Co(2+) for its optimal activity and stability. Overall, 52.2% conversion of D-glucose to D-fructose was achieved by TnapXI. Thus, it has a great potential for industrial applications.

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Section: Discussionsupporting

confidence: 82%

Cloning, purification, and characterization of xylose isomerase from Thermotoga naphthophila RKU‐10

Fatima

Aftab

Haq

2016

J. Basic Microbiol.

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“…Many microbial strains, including Streptomyces sp. CH7 [17], Lactobacillus bifermentans [18], Bacillus coagulans [19], Streptomyces murinus, Hyperthermophilic Thermotoga [20], and Pseudomonas hydrophila [21], have been reported to produce glucose isomerase. Because of the increased global demand for HFCS, the level of GIase production has gained considerable attention, especially in the food and beverages industry.…”

Section: Owingmentioning

confidence: 99%

Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup

2021

IJEAB

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High fructose syrup is a sweetener that can replace sucrose, which is widely used in the food and beverage industries. In this study, codon-optimized Actinoplanes missouriensis CICIM B0118 (A) derived glucose isomerase heterologous expression was realized in the food-grade strain Bacillus Subtilis 168, and the recombinant Bacillus Subtilis 168/pMA5-xylA was successfully constructed. The whole-cell bioconversion system of D-glucose isomerization to the biosynthesis of D-fructose was optimized. The results showed that the concentration of biocatalysts was DWC 40 g/L, and the concentration of substrate D-glucose was 180 g/L, Mg2+ concentration 10 mmol/L, Co2+ concentration 1 mmol/L, continuous conversion at 70℃、220 R/min for 18 h, D-fructose concentration reached 103.32 g/L, the conversion rate was 57.4%, realizing the high fructose syrup one-step safe biosynthesis. This research provided an experimental and theoretical basis for the industrialized and safe biosynthesis of high fructose syrup and had an important reference value.

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“…Similarly, a thermophilic class I enzyme from Thermobifida fusca MBL 10,003 showed Mr = 43.9 kDa (Kasumi et al 2012). Among class I, mesophilic enzyme from Streptomyces sp.CH7 has Mr = 43.6 kDa (Chanitnun and Pinphanichakarn 2012), while hyperthermophilic, class II enzyme from Thermotoga maritima MSB8 has Mr = 45 kDa (Brown et al 1993). It was previously reported that a ~ 1.3 kb (1320 bp) gene (xyl-A) fragment of E. coli K12 encoding for XI of 440 amino acid residues long has a Mr = 44 kDa (Schellenberg et al 1984).…”

Section: Purification Of Ecxi-bl21mentioning

confidence: 99%

Production, purification and physicochemical characterization of D-xylose/glucose isomerase from Escherichia coli strain BL21

Fatima

Javed

2020

3 Biotech

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Cell lysate of Escherichia coli strain BL21 showed significant D-glucose isomerase activity. The rate of glucose conversion was increased up to 40% when cells were induced with 1% D-xylose. E. coli BL21 xylose isomerase (ECXI-BL21) was purified to homogeneity, up to 1.9-fold with overall 10.88% enzyme yield by heat shock, salting out and electro-elution. The molecular mass of ECXI-BL21 was estimated as 43.9 kDa on SDS-PAGE. pH opt. and T opt. of the enzyme were calculated as 7.0 and 50 °C, respectively. Activation energy (E a ) of ECXI-BL21 was 45 kJ/mol. Enzyme was stable from 30 to 55 °C and at pH range 6.0-8.0. ECXI-BL21 (holo) was activated by 10 mM magnesium (35%), 0.5 mM cobalt (20%) and manganese (25%), and 0.5/10 mM Mn 2+ /Mg 2+ (50%) and Co 2+ /Mg 2+ (30%) as compared to ECXI-BL21 (apo) . Catalytic affinity (K m ) of ECXI-BL21 for D-glucose was calculated as 0.82 mM, while maximum velocity (V max ) of the reaction D-glucose (aldo) ⇌ D-fructose (keto) was 108 μmol/mg/min. D-fructose formed was identified on silica gel plate. This thermophilic enzyme, T m = 75 °C, has great potential for high fructose syrup production used in food and soft drink industries.

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Glucose(xylose) isomerase production by Streptomyces sp. CH7 grown on agricultural residues

Cited by 19 publications

References 27 publications

Cloning, purification, and characterization of xylose isomerase from Thermotoga naphthophila RKU‐10

Cloning, purification, and characterization of xylose isomerase from Thermotoga naphthophila RKU‐10

Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup

Production, purification and physicochemical characterization of D-xylose/glucose isomerase from Escherichia coli strain BL21

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