Glucose Utilization During Interictal Intervals in an Epilepsy Model Induced by Pilocarpine: A Qualitative Study

Scorza, Fúlvio Alexandre; Sanabria, E; Calderazzo, Lineu; Cavalheiro, Ésper A.

doi:10.1111/j.1528-1157.1998.tb01288.x

Cited by 29 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supporting our finding, Dal-Pizzol et al [4] found a decrease in TBARs levels in the hippocampus in the pilocarpine model during SRS and explained it by the neuronal loss [10,46] and hypometabolism observed in this structure [47]. Curcumin treatment resulted in a significant decrease in LP below control and pilocarpine-treated levels.…”

Section: Discussionsupporting

confidence: 83%

The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate

Ezz

Khadrawy²,

Noor

2011

Neurochem Res

View full text Add to dashboard Cite

Oxidative stress has been implicated to play a role in epileptogenesis and pilocarpine-induced seizures. The present study aims to evaluate the antioxidant effects of curcumin, Nigella sativa oil (NSO) and valproate on the levels of malondialdehyde, nitric oxide, reduced glutathione and the activities of catalase, Na⁺, K⁺-ATPase and acetylcholinesterase in the hippocampus of pilocarpine-treated rats. The animal model of epilepsy was induced by pilocarpine and left for 22 days to establish the chronic phase of epilepsy. These animals were then treated with curcumin, NSO or valproate for 21 days. The data revealed evidence of oxidative stress in the hippocampus of pilocarpinized rats as indicated by the increased nitric oxide levels and the decreased glutathione levels and catalase activity. Moreover, a decrease in Na⁺, K⁺-ATPase activity and an increase in acetylcholinesterase activity occurred in the hippocampus after pilocarpine. Treatment with curcumin, NSO or valproate ameliorated most of the changes induced by pilocarpine and restored Na⁺, K⁺-ATPase activity in the hippocampus to control levels. This study reflects the promising anticonvulsant and potent antioxidant effects of curcumin and NSO in reducing oxidative stress, excitability and the induction of seizures in epileptic animals and improving some of the adverse effects of antiepileptic drugs.

show abstract

Section: Discussionsupporting

confidence: 83%

The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate

Ezz

Khadrawy²,

Noor

2011

Neurochem Res

View full text Add to dashboard Cite

show abstract

“…The increased metabolic rate in LP during the interictal period may be a result of the activation of cerebral circuits controlling SRSs initiation and/or generalization 6 . The chronic epileptic conditions may be associated with increased inhibitory influences during the interictal state, which could reflect reactive homeostatic mechanisms to prevent seizure initiation or restrict ictal propagation once epileptogenesis occurs 13 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, these differences may be particularly important for understanding the triggering and spreading mechanisms underlying epileptic activity during status epilepticus and recurrent seizures 6 . Our results reinforce those considerations since we showed that LP lesion by ibotenic acid in chronic rats results in an increase of seizures frequency.…”

Section: Discussionmentioning

confidence: 99%

The contribution of the lateral posterior and anteroventral thalamic nuclei on spontaneous recurrent seizures in the pilocarpine model of epilepsy

Arida

Priel

Calderazzo

et al. 2002

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

-The pilocarpine model of epilepsy in rats is characterised by the occurrence of spontaneous seizures (SRSs) during the chronic period that recur 2-3 times per week during the whole animal life. In a previous study on brain metabolism during the chronic period of the pilocarpine model it was possible to observe that, among several brain structures, the lateral posterior thalamic nuclei (LP) showed a strikingly increased metabolism. Some evidences suggest that the LP can participate in an inhibitory control system involved in the propagation of the seizures. The aim of the present study was to verify the role of LP in the expression and frequency of spontaneous seizures observed in the pilocarpine model. Ten adult male rats presenting SRSs were monitored for behavioural events by video system one month before and one month after LP ibotenic acid lesion. Another group of chronic epileptic rats (n=10) had the anteroventral thalamic nuclei (AV) lesioned by ibotenic acid. After the surgical procedure, the animals were sacrified and the brains were processed for histological analysis by the Nissl method. The LP group seizure frequency was 3.1±1.9 before ibotenic acid injection and showed an increase (16.3±7.2 per week) after LP lesion. No changes in SRSs frequency were observed in the AV group after ibotenic lesion in these nuclei. These results seem to suggest that LP play a role in the seizure circuitry inhibiting the expression of spontaneous seizures in the pilocarpine model. KEY WORDS: pilocarpine, lateral posterior thalamic nuclei.A contribuição dos núcleos talâmico lateral posterior e anteroventral nas crises espontâneas e recorrentes A contribuição dos núcleos talâmico lateral posterior e anteroventral nas crises espontâneas e recorrentes A contribuição dos núcleos talâmico lateral posterior e anteroventral nas crises espontâneas e recorrentes A contribuição dos núcleos talâmico lateral posterior e anteroventral nas crises espontâneas e recorrentes A contribuição dos núcleos talâmico lateral posterior e anteroventral nas crises espontâneas e recorrentes no modelo de epilepsia induzido pela pilocarpina no modelo de epilepsia induzido pela pilocarpina no modelo de epilepsia induzido pela pilocarpina no modelo de epilepsia induzido pela pilocarpina no modelo de epilepsia induzido pela pilocarpina RESUMO -O modelo de epilepsia induzido pela pilocarpina é caracterizado pela ocorrência de crises espontâneas e recorrentes (CERs) durante o período crônico, em uma frequência de 2-3 episódios semanais durante toda a vida do animal. Um estudo prévio do metabolismo cerebral durante o período crônico do modelo da pilocarpina mostra que entre as diferentes estruturas cerebrais,o núcleo talâmico lateral posterior (LP) apresentou um significante aumento em seu metabolismo. Evidências sugerem que o LP pode participar como um sistema de controle inibitório na propagação das crises. O objetivo deste estudo foi verificar o papel do LP na expressão e frequência das CERs no modelo de epilepsia induzido pela pilocarpina. De...

show abstract

“…The anatomic substrates activated during seizure activity differ from those activated during interictal intervals 23 . We have recently showed that increased metabolic rate in the lateral posterior thalamic nuclei during the interictal period may be a result of the activation of cerebral circuits controlling SRSs initiation and/or generalisation.…”

Section: Discussionmentioning

confidence: 99%

Glucose utilisation during status epilepticus in an epilepsy model induced by pilocarpine: a qualitative study

Arida

Priel

Calderazzo

et al. 2002

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

-Status epilepticus (SE) is a medical emergency and it is associated to brain damage. 2-deoxy-[14 C] glucose (2-DG) procedure has been used to measure the alterations in the functional activity of the brain induced by various pharmacological and toxicological agents. The aim of this study was to determine which changes occur in the seizure anatomic substrates during the SE induced by pilocarpine (PILO) using [ 14 C]-2 deoxyglucose functional mapping technique. Wistar male adult rats were submitted to SE PILO-induced for 6h and received [ 14 C] 2-deoxyglucose injection via jugular vein 45 min before the 6 th hour of SE. The control animals were submitted to all procedures but received saline and not pilocarpine. Brain sections were prepared and exposed X-ray film about seven days. The optical density of each region was obtained using a solid state digital analyser. The analysis revealed that 14 C-2DG utilisation was pronounced in the SE rats on the areas corresponding to the hippocampal formation (+50.6%), caudate-putamen (+30.6%), frontoparietal cortex (+32.2%), amygdala (+31.7%), entorrinal cortex (+28.2%), thalamic nucleus (+93.5%), pre-tectal area (+50.1%) and substantia nigra (+50.3%) when compared to control. Our results suggest that the different activation levels of the distinct structures may be particularly important for understanding triggering and spreading mechanisms underlying epileptic activity during status epilepticus.KEY WORDS: deoxyglucose, pilocarpine, seizure, status epilepticus.Utilização de glicose durante o estado de mal epiléptico no modelo de epilepsia induzido pela pilocarpina: um estudo qualitativo RESUMO -O estado de mal epiléptico (SE) é uma emergência médica e está associado a lesão cerebral. O procedimento da [ 14 C] desoxiglicose tem sido utilizado para avaliar as alterações da atividade funcional cerebral induzidas por agentes farmacológicos e toxicológicos. O objetivo deste estudo foi verificar as alterações metabólicas do cérebro de ratos durante o SE induzido pela pilocarpina, para tanto, utilizamos a técnica de mapeamento funcional da [ 14 C] desoxiglicose. Ratos machos da raça Wistar foram submetidos ao SE induzido pela pilocarpina durante período de 6 horas; 45 minutos antes de se completar 6 horas de SE, tais animais receberam uma injeção de [ 14 C] desoxiglicose por via venosa (veia jugular). Os animais pertencentes ao grupo controle foram submetidos aos mesmos procedimentos, no entanto, receberam solução salina e não pilocarpina. As fatias cerebrais foram preparadas e expostas em filme de raioX por um período de sete dias. A análise da densidade óptica de cada região foi obtida por analisador digital de estado sólido. Tal análise revelou aumento no consumo de glicose durante o SE nas seguintes regiões: formação hipocampal (+50,6%), núcleo caudadoputamen (+30,6%), córtex frontoparietal (+32,2%), amigdala (+31,7%), córtex entorrinal (+28,2%), complexo talâmico (+93,5%), área pré-tectal (+50,1%) e substância negra (+50,3%), quando comparadas com os animais pertence...

show abstract

Glucose Utilization During Interictal Intervals in an Epilepsy Model Induced by Pilocarpine: A Qualitative Study

Cited by 29 publications

References 17 publications

The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate

The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate

The contribution of the lateral posterior and anteroventral thalamic nuclei on spontaneous recurrent seizures in the pilocarpine model of epilepsy

Glucose utilisation during status epilepticus in an epilepsy model induced by pilocarpine: a qualitative study

Contact Info

Product

Resources

About