Glucose utilization by Streptomyces griseus

Abstract

SUMMARY: Glucose utilization by a strain of Streptomyces griseus was studied. Under highly aerobic conditions, glucose was converted mainly to structural material and CO,, but under restricted aeration, lactic acid was formed. Pyruvic acid was also formed during the stages of most rapid growth. The metabolism of glucose was dependent upon the presence of phosphate, and the optimal hydrogenion concentration for both glucose oxidation and the rate of disappearance of inorganic phosphate was about pH 7. Phosphate esters, tentatively identified as glucose-1-phosphate and glucose-6-phosphate, were obtained in fluoride-inhibited systems. Glucose oxidation was depressed by M-sodium iodoacetate and Msodium arsenite but was stimulated by 10-2M-sodium arsenate ; 1 0 -3~-2 : 4-dinitrophenol and 10-sM-sodium azide had no effect. Streptomycin production was decreased by 3 x lO-*M-sodium arsenate but not by 10-2M-sodium fluoride or ~O-*Msodium iodoacetate. S. griseus metabolized members of the tricarboxylic acid cycle, although citrate and a-ketoglutarate gave much lower values of Qo, a t pH 7.3 than pyruvate, acetate, succinate, fumarate or malate. Ketoacids were produced in presence of arsenite from fumarate, malate, glucose, lactate, acetate, succinate, glutamate and citrate in descending order of yield. Except from fumarate, which yielded some material behaving like a-ketoglutarate, the product was chiefly p p v a t e .

Role of Acid Metabolism in Streptomyces coelicolor Morphological Differentiation and Antibiotic Biosynthesis

“…The acidosis and developmental defect of BZ2, similar to those of most other bald mutants, was dependent on glucose. Previous studies have shown that organic acid excretion reflects imbalance between glycolysis and TCA cycle activities (13) and can be induced by oxygen limitation (12). The citrate synthase mutant consumed glucose at slightly higher rates and was unable to oxidize glycolytic products via the TCA cycle.…”

Amino acid uptake profiling of wild type and recombinant Streptomyces lividans TK24 batch fermentations

“…In the present results, lactic acid accumulates during the entire growth phase. Although Streptomycetes are classified as strictly aerobic, their tendency to flocculate makes that the inside of the clumps grow probably micro-aerobically and produce lactate (Hockenhull et al, 1954;Borodina et al, 2005). Succinate production could also be caused by this oxygen limitation inside the kernels of the flocs but equally well may result from an imbalance of the carbon fluxes in the TCA cycle as shown by the WT experiment supplemented with 15 g/L casamino acids.…”

Genome-scale analysis of Streptomyces coelicolor A3(2) metabolism

“…The presence of lactate dehydrogenase in the sequenced genome of S. coelicolor A3(2) indicates that the organism should be capable of having a fully operational fermentative metabolism with lactate as a key fermentation product. Indeed, excretion of lactate has been observed for Streptomyces griseus grown under microaerophilic conditions (Hockenhull et al 1954). Two reasons were suggested to explain the anaerobic Streptomyces paradox: either the organisms are sensitive to the fermentation products or/and there are some essential reactions that require oxygen.…”