Glucose uptake rates of single E. coli cells grown in glucose-limited chemostat cultures

Natarajan, A.T.; Srienc, Friedrich

doi:10.1016/s0167-7012(00)00180-9

Cited by 54 publications

(51 citation statements)

References 12 publications

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“…However, export of NBD by unspecific cellular efflux systems is probable. To prevent degradation and fluorescence disappearance 2,4-dinitrophenol can be added to disrupt the energy balance necessary for ATP synthesis for glucose metabolism (54).…”

Section: Fluorescent Substratesmentioning

confidence: 99%

Viability states of bacteria—Specific mechanisms of selected probes

2010

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Single cell techniques like flow cytometry combined with viability staining can help to obtain information on viability states of bacteria. Many fluorescent dyes are available for this purpose and can be chosen according to the available excitation source, the species used, and the background of scientific questions and relevant specifications. Within this short overview, we focus on two diverse groups of bacteria: the gram2 Escherichia coli and representatives of the gram+ Mycobacterium to demonstrate differences and similarities in dye uptake principles, processing and binding. We call for attention to possible diverse responses of different species to various viability assays. The cell surface structure of bacteria and the chemical properties of fluorescent probes considerably determine the success of a certain staining practice. Particular focus was drawn on analysis of membrane integrity, uptake of substrates and transformation of fluorogenic substrates. '

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Section: Fluorescent Substratesmentioning

confidence: 99%

Viability states of bacteria—Specific mechanisms of selected probes

2010

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“…10,67 Often they are associated with chondroitin sulfate. 67,68 For analytical and bioanalytical qualitative or quantitative determinations of 3 (α + β) 8,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] one may use TLC as a simpler alternative to electrophoresis 34 or HPLC. 16 The procedure involves reacting 5 to 10 mg of powdered pharmaceutical preparation either with 1 or with 4a, in conditions described in the Experimental Part, followed by TLC analysis for qualitative analysis (one or two spots depending on the adsorbant and solvent) or by quantitative densitometry when the detection involves UV-Vis or fluorescence spectroscopy.…”

Section: Analysis Of Glucosamine In Pharmaceutical Preparationsmentioning

confidence: 99%

“…15 More recently, it was possible to provide evidence for the separated anomers by HPLC. 16 The mixture of anomers 3 is commercially available for visualizing the transport and cellular metabolism of glucose and its analogs, 8,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] and for the electrophoretic determination of glucosamine. …”

Section: Introductionmentioning

confidence: 99%

4-(D-Glucosamino)-7-nitrobenzoxadiazole: synthesis, anomers, spectra, TLC behavior, and applications

Bem¹,

Badea²,

Drăghici³

et al. 2008

Arkivoc

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A new synthesis of the known fluorescent compound 3 [4-(D-glucosamino)-7-nitro-2,1,3-benzoxadiazol-4-yl] is reported, starting from D-glucosamine and non-fluorescent 4-aryloxy-7-nitrobenzofurazans, 4a-e, 5. The α and β anomers are easily interconverted but can be separated by TLC (R f β > R f α). The non-fluorescent new congener 8 {2-[N-(2',4',6'-trinitrophenyl)-amino]-2-deoxy-D-glucose} and the related known compound 9 {2-[N-(2',4'-dinitrophenyl)-amino]-2-deoxy-D-glucose} have anomers that may be seen in NMR spectra, but are too rapidly interconverted for TLC separation. The UV-Vis and fluorescence spectra of 3 depend markedly on solvent polarity. The TLC method allows the analytical determination of glucosamine from pharmaceutical preparations by conversion into 3 and detection by its fluorescence.

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“…Recently, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, has become available (Yoshioka et al, 1996b) and has been used to assess glucose transport in vascular smooth muscle cells (Lloyd et al, 1999), Escherichia coli (Natarajan and Srienc, 2000;Yoshioka et al, 1996b), astrocytes (Loaiza et al, 2003), cardiomyocytes (Ball et al, 2002), enterocytes (Román et al, 2001), yeast Candida albicans (Yoshioka et al, 1996a), and pancreatic ␤-cells (Yamada et al, 2000). In the present study, we first validated the usefulness of 2-NBDG as a fluorescent tracer to trace glucose utilization in cultured neurons and astroglia and then applied it in studies in whole animals.…”

mentioning

confidence: 99%

Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo

Itoh

Abe

Takaoka

et al. 2004

J Cereb Blood Flow Metab

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Summary:Glucose is the major energy source the adult brain utilizes under physiologic conditions. Recent findings, however, have suggested that neurons obtain most of their energy from the oxidation of extracellular lactate derived from astroglial metabolism of glucose transported into the brain from the blood. In the present studies we have used 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose, which is often used to trace glucose utilization in neural tissues, to examine glucose metabolism in neurons in vitro and in vivo. Cultured neurons and astroglia were incubated with 2-NBDG for up to 15 minutes, and nonmetabolized 2-NBDG was washed out. We found that fluorescence intensity increased linearly with incubation time in both neurons and astroglia, indicating that both types of brain cells could utilize glucose as their energy source in vitro. To determine if the same were true in vivo, Sprague-Dawley rats were injected intravenously with a pulse bolus of 2-NBDG and decapitated 45 minutes later. Examination of brain sections demonstrated that phosphorylated 2-NBDG accumulated in hippocampal neurons and cerebellar Purkinje cells, indicating that neurons can utilize glucose in vivo as energy source.

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Glucose uptake rates of single E. coli cells grown in glucose-limited chemostat cultures

Cited by 54 publications

References 12 publications

Viability states of bacteria—Specific mechanisms of selected probes

Viability states of bacteria—Specific mechanisms of selected probes

4-(D-Glucosamino)-7-nitrobenzoxadiazole: synthesis, anomers, spectra, TLC behavior, and applications

Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo

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