Glucose uptake by the cellulolytic ruminal anaerobe Bacteroides succinogenes

Franklund, C V; Glass, Thomas L.

doi:10.1128/jb.169.2.500-506.1987

Cited by 56 publications

(28 citation statements)

References 33 publications

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“…Negligible glucose PTS activity was also reported for B. thetaiotaomicron (Hylemon et al 1977) and B. (Fibrobacter) succino9enes (Martin and Russell 1986;Franklund and Glass 1987).…”

Section: Resultsmentioning

confidence: 91%

Factors affecting glucose uptake by the ruminal bacterium Bacteroides ruminicola

Martin

1992

Appl Microbiol Biotechnol

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Glucose uptake by whole cells of Bacteroides rurninicola B14 is constitutive. Potassium concentrations between 10 and 150 mM stimulated uptake over fourfold, while sodium had little effect on uptake. The involvement of potassium in glucose uptake by B. ruminicola was supported by strong inhibition of uptake by the ionophores valinomycin, lasalocid, and monensin. The electron transport inhibitor antimycin A had little effect on uptake, but menadione and acriflavine inhibited uptake by 30 and 48%, respectively. Potent inhibitors of uptake included oxygen, p-chloromercuribenzoate, HgCI2, and o-phenanthroline. Sodium arsenate decreased uptake by 40%, suggesting that a highenergy phosphate compound and possibly a binding protein may be involved in glucose uptake. The protonophores carbonyl cyanide m-chlorophenylhydrazone and 2,4-dinitrophenol inhibited glucose uptake by 37 and 22%, respectively. Little change in uptake activity was observed at extracellular pH values between 4.0 and 8.0. Excess (10 mM) cellobiose, maltose, and sucrose inhibited glucose uptake less than 15%. High levels (0.15% w/v) of p-coumaric acid and vanillin decreased uptake by 32 and 37%, respectively, while 0.15% ferulic acid decreased uptake by 15%.

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“…Negligible glucose PTS activity was also reported for B. thetaiotaomicron (Hylemon et al 1977) and B. (Fibrobacter) succino9enes (Martin and Russell 1986;Franklund and Glass 1987).…”

Section: Resultsmentioning

confidence: 91%

Factors affecting glucose uptake by the ruminal bacterium Bacteroides ruminicola

Martin

1992

Appl Microbiol Biotechnol

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“…An ABC protein associated with a xylan metabolism operon has been identified based on DNA sequence data in R. flavefaciens (21). By contrast, F. succinogenes utilizes a Na ϩ electrochemical gradient for uptake of glucose (195) and cellobiose (412), and the Na ϩ requirement for growth on cellulose suggests that Na ϩ may be the driving force for uptake of cellodextrins as well (740).…”

Section: Physiology Of Cellulolytic Microorganismsmentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,020

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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“…In addition, Russell also showed that valinomycin collapsed ⌬⌿ in F. succinogenes (22). Furthermore, when used alone CCCP and monensin inhibited respectively glucose and cellobiose uptake by F. succinogenes (18,19).…”

Section: Calculation Of Intracellular Sodium Concentration By Using Imentioning

confidence: 98%

“…More recently, Simor et al (16) compared the effect of Dy(TTHA) 3Ϫ , Tm(DOTP) 5Ϫ , and Dy(PPP i ) 2 7Ϫ on isolated perfused rat hearts and showed that Tm(DOTP) 5Ϫ was the most appropriate under their experimental conditions. F. succinogenes is a major rumen bacterium which requires sodium for growth and for glucose and cellobiose uptake (17)(18)(19), suggesting that sodium may play an important role in the bioenergetics of the bacterium. In order to measure sodium transmembrane gradient in this bacterium and its variation according to extracellular sodium concentrations and pH, we developed a methodology to measure accurately Na ϩ internal concentrations in F. succinogenes S85 by 23 Na NMR.…”

mentioning

confidence: 99%