Glucose transport machinery reconstituted in cell models

Hansen, Jesper S.; Elbing, Karin; Thompson, James R.; Malmstadt, Noah; Lindkvist‐Petersson, Karin

doi:10.1039/c4cc08838g

Cited by 33 publications

(30 citation statements)

References 48 publications

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“…8,15 The technique has since expanded the possibility to study complex lipid-protein interactions, 16,17 vesicles for applications in drug delivery 18 and use of vesicles as micro-reactors. 19,20 However no studies have reported multicompartment formation in vesicles using this methodology.…”

Section: Introductionmentioning

confidence: 99%

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport

et al. 2020

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“…A very recent study employing channel proteins was conducted by Hansen et al Giant liposomes reconstituted with GLUT1 glucose transporter (GLUT) were assembled and their ability to encapsulate the enzyme tandem pair of GOx and HRP was evaluated. GLUT‐reconstituted giant liposomes were assembled employing a hydrogel‐assisted swelling procedure, and the ability of GLUT to allow for β ‐d ‐glucose transport was evaluated by monitoring the enzymatic activity of the system.…”

Section: Micro/nanosized Single‐compartment Enzymatic Reactorsmentioning

confidence: 99%

“…Different architectures that meet the aforementioned requirements have been considered for the design of artificial organelles, being liposomes and polymeric‐based systems the most advanced carriers both regarding structure but also functionality . Nonetheless, recently, other types of architectures made of inorganic materials such as silica are emerging as new systems for the construction of artificial organelles …”

Section: Introductionmentioning

confidence: 99%

Recent Progress in Micro/Nanoreactors toward the Creation of Artificial Organelles

Godoy-Gallardo

York-Durán

Hosta‐Rigau

2017

Adv Healthcare Materials

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Artificial organelles created from a bottom up approach are a new type of engineered materials, which are not designed to be living but, instead, to mimic some specific functions inside cells. By doing so, artificial organelles are expected to become a powerful tool in biomedicine. They can act as nanoreactors to convert a prodrug into a drug inside the cells or as carriers encapsulating therapeutic enzymes to replace malfunctioning organelles in pathological conditions. For the design of artificial organelles, several requirements need to be fulfilled: a compartmentalized structure that can encapsulate the synthetic machinery to perform an enzymatic function, as well as a means to allow for communication between the interior of the artificial organelle and the external environment, so that substrates and products can diffuse in and out the carrier allowing for continuous enzymatic reactions. The most recent and exciting advances in architectures that fulfill the aforementioned requirements are featured in this review. Artificial organelles are classified depending on their constituting materials, being lipid and polymer-based systems the most prominent ones. Finally, special emphasis will be put on the intracellular response of these newly emerging systems.

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“…For their reconstitution, solubilized membrane proteins can be dissolved in warm, molten agarose gels (Figure 2 B) (Hansen et al, 2013 , 2015 ; Gutierrez and Malmstadt, 2014 ). In this technique, precipitation of the detergents is prevented at a dilution below the cmc.…”

Section: Rehydration Of Protein-containing Agarosementioning

confidence: 99%

“…Upon rehydration of the lipids, protein-containing liposomes form from the surface of the protein-containing agarose. The proteins successfully used in this approach so far were aquaporin-Z, bacteriorhodopsin, and SoPIP2 (Hansen et al, 2013 ), as well as the glucose transporter GLUT1 (Hansen et al, 2015 ) and the human serotonin receptor 5-HT1A (Gutierrez and Malmstadt, 2014 ).…”

Section: Rehydration Of Protein-containing Agarosementioning

confidence: 99%

Compartmentalization and Transport in Synthetic Vesicles

Schmitt

Lippert

Bonakdar

et al. 2016

Front. Bioeng. Biotechnol.

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Nanoscale vesicles have become a popular tool in life sciences. Besides liposomes that are generated from phospholipids of natural origin, polymersomes fabricated of synthetic block copolymers enjoy increasing popularity, as they represent more versatile membrane building blocks that can be selected based on their specific physicochemical properties, such as permeability, stability, or chemical reactivity. In this review, we focus on the application of simple and nested artificial vesicles in synthetic biology. First, we provide an introduction into the utilization of multicompartmented vesosomes as compartmentalized nanoscale bioreactors. In the bottom-up development of protocells from vesicular nanoreactors, the specific exchange of pathway intermediates across compartment boundaries represents a bottleneck for future studies. To date, most compartmented bioreactors rely on unspecific exchange of substrates and products. This is either based on changes in permeability of the coblock polymer shell by physicochemical triggers or by the incorporation of unspecific porin proteins into the vesicle membrane. Since the incorporation of membrane transport proteins into simple and nested artificial vesicles offers the potential for specific exchange of substances between subcompartments, it opens new vistas in the design of protocells. Therefore, we devote the main part of the review to summarize the technical advances in the use of phospholipids and block copolymers for the reconstitution of membrane proteins.

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Glucose transport machinery reconstituted in cell models

Cited by 33 publications

References 48 publications

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport

Recent Progress in Micro/Nanoreactors toward the Creation of Artificial Organelles

Compartmentalization and Transport in Synthetic Vesicles

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