Glucose transport as rate-limiting step in the growth of <i>Escherichia coli</i> on glucose

Herbert, D.; Kornberg, H. L.

doi:10.1042/bj1560477

Cited by 57 publications

(32 citation statements)

References 12 publications

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“…For instance, this has been reported for the phosphoenolpyruwww.annualreviews.org/aronline Annual Reviews ate-dependent sugar phosphotransferase system involved in glucose uptake in E. coli (51) and other organisms (19), the uptake rate of the amino acids alanine, glutamate, and arginine in Streptomyces hydrogenans (3), and sugars in Thiobacillus A2 (136). Such a control system could not only ensure that the rate of transport of s.ubstrates is sufficiently high at higher growth rates, but conceivably might also, b~y derepression of the synthesis of carriers at very low growth rates, scavenge substrate molecules when their concentration is diminishingly low.…”

Section: Regulation Of Substrate-uptake Systemsmentioning

confidence: 99%

Physiological Responses to Nutrient Limitation

Harder¹,

Dijkhuizen²

1983

Annu. Rev. Microbiol.

341

166

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Section: Regulation Of Substrate-uptake Systemsmentioning

confidence: 99%

Physiological Responses to Nutrient Limitation

Harder¹,

Dijkhuizen²

1983

Annu. Rev. Microbiol.

341

166

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“…Culture liquid (50 ml) was withdrawn from the chemostat and immediately incubated at 37 OC in a pre-warmed 200 ml Erlenmeyer flask. No pre-treatment was used because washing has been reported to reduce the capacity of bacteria to incorporate glucose (Herbert & Kornberg, 1976). Aeration was ensured by mixing the culture with a magnetic stirrer at 500 r.p.m.…”

Section: Methodsmentioning

confidence: 99%

Is Escherichia coli growing in glucose-limited chemostat culture able to utilize other sugars without lag?

Lendenmann

Egli²

1995

Microbiology

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It was investigated whether Escherichia coli cultured in a glucose-limited chemostat is able to grow with a series of sugars whose utilization is normally repressed during batch growth with glucose. Cells growing at dilution rates of 02,03 and 0 6 h-' were able to immediately utilize and grow with fructose, mannose, maltose and ribose. Galactose was transported instantaneously but growth started only after a considerable lag. Arabinose was the only sugar tested which was neither transported nor utilized immediately. The results give experimental evidence that in vivo catabolite repression by glucose is absent at the very low glucose concentrations present in chemostat culture. Additionally, the results demonstrate that chemostat-grown cells of E. coli are able to substitute glucose as a carbon and energy source by several other sugars with no lag period.

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“…The residual glucose concentration of the supernate was determined by the method described by Kingsley and Getchell [24]. The rate of glucose consumption was calculated from the equation of Herbert and Kornberg [25]:…”

Section: Measurement Of Glucose Consumption Rates Of the Microbial Spmentioning

confidence: 99%

Competition for glucose between Candida albicans and oral bacteria grown in mixed culture in a chemostat

2000

Journal of Medical Microbiology

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The competition for glucose as a growth-limiting substrate between Candida albicans and a mixed community of oral bacteria was investigated. A chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. A mixed population of oral bacteria was established and after a steady state had been reached the chemostat was inoculated with C. albicans. Seven bacterial species ± Streptococcus sanguis, S. sobrinus, S. mitis, Lactobacillus casei, Veillonella dispar, Eubacterium saburreum and Fusobacterium nucleatum ± were able to establish stable populations under glucose-limiting conditions. The yeast was unable to grow with the bacteria under glucose limitation. Only three bacterial species, S. sobrinus, L. casei and E. saburreum, became established under glucose-excess conditions. C. albicans was also able to become established in the glucose-excess chemostat and could grow and maintain a steady state in a mixed culture with these organisms. L. casei, S. mitis and S. sobrinus had faster glucose consumption rates than C. albicans. All the bacteria, except for F. nucleatum, had maximum speci®c growth rates higher than C. albicans. The results suggest that glucose may act as a growth-limiting substrate for C. albicans in the establishment and growth of the yeast in a mixed community of oral bacteria.

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Glucose transport as rate-limiting step in the growth of Escherichia coli on glucose

Abstract: Over a wide range of growth rates, two strains of Escherichia coli growing aerobically in continuous culture under glucose limitation utilized glucose at rates identical with those at which cells harvested from the chemostats transported [14C]glucose.

Cited by 57 publications

References 12 publications

Physiological Responses to Nutrient Limitation

Physiological Responses to Nutrient Limitation

Is Escherichia coli growing in glucose-limited chemostat culture able to utilize other sugars without lag?

Competition for glucose between Candida albicans and oral bacteria grown in mixed culture in a chemostat

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