Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein

Tolosa, Leah; Gryczyński, Ignacy; Eichhorn, Lisa R.; Dattelbaum, Jonathan D.; Castellano, Felix N.; Rao, Govind; Lakowicz, Joseph R.

doi:10.1006/abio.1998.2974

Cited by 190 publications

(160 citation statements)

References 40 publications

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“…The first one is inconvenience and the required compliance by patients, while the second is that this is not a continuous monitoring method. Despite intensive efforts [1][2][3][4][5][6][7][8][9][10][11], no method is currently available for the continuous non-invasive monitoring of blood glucose.…”

Section: Introductionmentioning

confidence: 99%

A Glucose Sensing Contact Lens: A Non-Invasive Technique for Continuous Physiological Glucose Monitoring

Badugu

Lakowicz

Geddes

2003

Journal of Fluorescence

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105

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We have developed a range of glucose sensing contact lenses, using a daily, disposable contact lens embedded with newly developed boronic acid containing fluorophores. Our findings show that our approach may be suitable for the continuous monitoring of tear glucose levels in the range 50-1000 µM, which typically track blood glucose levels, which are ≈5-10 fold higher. Our non-invasive approach may well offer an alternative solution to current invasive glucose monitoring techniques for diabetes, such as "finger pricking."

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Section: Introductionmentioning

confidence: 99%

A Glucose Sensing Contact Lens: A Non-Invasive Technique for Continuous Physiological Glucose Monitoring

Badugu

Lakowicz

Geddes

2003

Journal of Fluorescence

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105

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“…The labeled GBP responds to increasing glucose concentrations by a decrease in fluorescence intensity. 34 …”

Section: Biosensormentioning

confidence: 99%

Passive Diffusion of Transdermal Glucose

Kanjananimmanont

Mupparapu

et al. 2014

J Diabetes Sci Technol

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The motivation for this study was to determine if a statistically significant correlation exists between blood glucose (BG) and transdermal glucose (TG) collected by passive diffusion. A positive outcome will indicate that noninvasive passive TG diffusion is a painless alternative to collecting blood through a break on the skin. Sampling involves placing a small volume of buffer solution on the surface of membrane or skin for 5 minutes. The sample is then assayed with fluorescent GBP. In vitro testing was done on regenerated cellulose and a porcine skin model to determine diffusion of standard glucose solutions. In vivo testing was done on a healthy subject and a subject with type 2 diabetes. Glucose diffused readily through the regenerated cellulose membrane with good correlation between surface and internal glucose concentrations (R 2 = .997). But the porcine skin model required a surface prewash to achieve the same good correlation R 2 = .943). Based on this, an optimum prewash step was determined for the in vivo studies. The resulting correlation coefficients between TG and BG after a 15-minute prewash in a healthy subject and type 2 subject were .87 and .93, respectively. Removal of the extraneous glucose in the skin by prewashing was an important step in achieving good correlation between TG and BG. The results suggest that passive collection of TG is a noninvasive alternative to current practice of breaking the skin. Further studies are under way to determine the lag time between TG and BG and for the sampling protocol to be more amenable to point-ofcare application.

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“…To this end, many technologies have been developed over the past 20 years in an attempt to provide a technology which promises both non-invasive and continuous physiological glucose monitoring. These include near infrared spectroscopy [3,4], optical rotation [5,6], colorimetric [7,8] and fluorescence detection [9][10][11][12][13], to name but just a very few. Recently we have seen the launch of the new GlucoWatch, which approved by the FDA in 2001, is the first step towards both the continuous and non-invasive monitoring of physiological glucose.…”

Section: Introductionmentioning

confidence: 99%

Ophthalmic Glucose Monitoring Using Disposable Contact Lenses—A Review

Badugu

Lakowicz

Geddes

2004

Journal of Fluorescence

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We have developed a range of disposable and colorless tear glucose sensing contact lenses, using offthe-shelf lenses embedded with new water soluble, highly fluorescent and glucose sensitive boronic acid containing fluorophores. The new lenses are readily able to track tear glucose levels and therefore blood glucose levels, which are ideally suited for potential use by diabetics. The fluorescence responses from the lenses can be monitored using simple excitation and emission detection devices. The novelty of our approach is two fold. Firstly, the notion of sensing extremely low glucose concentrations in tears, which track blood levels, by our contact lens approach, and secondly, the unique compatibility of our new glucose signaling probes with the internal mildly acidic contact lens environment. The new lenses are therefore ideal for the non-invasive and continuous monitoring of tear glucose, with about 15-min response time, and a measured shelf life in excess of 3 months. In this review article, we show that fluorescence based signaling using plastic disposable lenses, which have already been industrially optimized with regard to vision correction and oxygen/analyte permeability etc, may a notable alternative to invasive and random finger pricking, the most widely used glucose monitoring technology by diabetics.

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Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein

Cited by 190 publications

References 40 publications

A Glucose Sensing Contact Lens: A Non-Invasive Technique for Continuous Physiological Glucose Monitoring

A Glucose Sensing Contact Lens: A Non-Invasive Technique for Continuous Physiological Glucose Monitoring

Passive Diffusion of Transdermal Glucose

Ophthalmic Glucose Monitoring Using Disposable Contact Lenses—A Review

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