Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

Esposito, Rosario; Ventura, Bartolomeo Della; Nicola, S. De; Altucci, C.; Velotta, R.; Mita, D.G.; Lepore, Maria

doi:10.3390/s110403483

Cited by 30 publications

(18 citation statements)

References 40 publications

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“…A similar result has been demonstrated by Esposito et al [44], in the presence of glucose oxidase instead of ZnO:rgo composites. The formation of gluconic acid can be experimentally verified by analysing the FTIR spectra of the pure glucose, GZ3 and glucosetreated GZ3 (nanowire) to confirm the evolution of gluconic acid and H 2 O 2 during the decomposition of glucose.…”

Section: Glucose Sensingsupporting

confidence: 80%

Morphological tuned preparation of zinc oxide: reduced graphene oxide composites for non-enzymatic fluorescence glucose sensing and enhanced photocatalysis

Sivalingam

Karthikeyan

2016

Appl. Phys. A

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Zinc oxide: reduced graphene oxide (ZnO:rgo) composites with varying ZnO morphologies have been synthesized towards the application of non-enzymatic fluorescence (FL) glucose sensor and photocatalysis for methylene blue (MB) degradation. The phase structure of ZnO has confirmed by X-ray diffraction studies, and the band gap calculations were done by UV absorption spectra. Scanning electron microscope and Raman spectra revealed the morphological change and the vibrational studies of the prepared samples, respectively. The quenching of the FL emission band of ZnO:rgo composite sample confirmed the transfer of electrons from ZnO to rgo which inhibit the exciton recombination process. The non-enzymatic FL glucose sensing was carried out by the addition of dextrose glucose (D-glucose) into the ZnO:rgo composite solution, which shows strong relationship between glucose concentration and the FL intensity. The photocatalytic studies showed that composite with high surface to volume ratio exhibits a maximum degradation of MB over 93 %. Our combined results ensured that the ZnO:rgo composites with varying morphologies could be an effective system for applications such as FL-based glucose sensing and photocatalytic degradation.

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Section: Glucose Sensingsupporting

confidence: 80%

Morphological tuned preparation of zinc oxide: reduced graphene oxide composites for non-enzymatic fluorescence glucose sensing and enhanced photocatalysis

Sivalingam

Karthikeyan

2016

Appl. Phys. A

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“…The use of this support for glucose oxidase (GOD) immobilization received particular attention during the years from many research groups in order to design amperometric [5][6][7][8][9][10] or optical biosensors [11][12][13]. The great interest in GOD based sensors is due to enzyme ability to interact with glucose, whose quantification is among the most important present analytical tasks [14].…”

Section: Introductionmentioning

confidence: 99%

Enzyme distribution and secondary structure of sol–gel immobilized glucose oxidase by micro-attenuated total reflection FT-IR spectroscopy

Delfino

Portaccio

Ventura

et al. 2013

Materials Science and Engineering: C

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“…It has been claimed that sol-gel encapsulation is a good method for GOx fluorescence measurement [74,75]; in our experience, however [76], it does not seem to be fully appropriate. We have observed that, although GOx-FS (GOx linked to FS) seems initially to be immobilized in the sol-gel, the GOx-FS subsequently begins to be leached from the matrix and in less than two weeks the solgel loses more than 50% of the initial GOx-FS.…”

Section: Sensors Based On Gox Fluorescencementioning

confidence: 80%

Reagentless fluorescent biosensors based on proteins for continuous monitoring systems

et al. 2012

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There is a lack of commercially available efficient and autonomous systems capable of continuous monitoring of (bio)chemical data for clinical, environmental, food, or industrial samples. The weakest link in the design of these systems is the (bio)chemical receptor (bCR). The bCR should have transducer ability, the recognition event should be a single reaction, and the bCR should be easily regenerated. Transport proteins and enzymes are well placed as bCR for optical continuous monitoring systems (OCMS). In this paper we review quantitative aspects and the main transducer strategies which have been developed for transport proteins, using periplasmic binding proteins (linking an environmentally sensitive fluorophore or FRET between two fluorophores) and concanavalin A (competitive reversible assays) as representative examples. Efficient immobilization systems and implementation in OCMS are also reviewed. Some kinds of enzymes can fulfil the necessary requirements to be appropriate bCR. Strategies using flavoenzymes chemically modified with fluorophores can be successfully implemented in OCMS and they are, in our opinion, the most appropriate option.

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Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

Cited by 30 publications

References 40 publications

Morphological tuned preparation of zinc oxide: reduced graphene oxide composites for non-enzymatic fluorescence glucose sensing and enhanced photocatalysis

Morphological tuned preparation of zinc oxide: reduced graphene oxide composites for non-enzymatic fluorescence glucose sensing and enhanced photocatalysis

Enzyme distribution and secondary structure of sol–gel immobilized glucose oxidase by micro-attenuated total reflection FT-IR spectroscopy

Reagentless fluorescent biosensors based on proteins for continuous monitoring systems

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