Glucose-rich polysaccharide from dried ‘Shixia’ longan activates macrophages through Ca2+ and CR3- mediated MAPKs and PI3K-AKT pathways

Lan, Haibo; Cheng, Yi; Mu, Jingjing; Huang, Yanfen; Chen, Huifang; Zhao, Lei; Wang, Kai; Hu, Zhuoyan

doi:10.1016/j.ijbiomac.2020.11.040

Cited by 27 publications

(13 citation statements)

References 47 publications

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“…It was similar to previous studies of Lan et al. (2021), where they extracted a polysaccharide named LPsx from dried “Shixia” longan and found that NO production was positively correlated with the concentration of LPsx, suggesting that LPsx promoted NO secretion by macrophages. Similarly, Li et al.…”

Section: Resultssupporting

confidence: 91%

“…All of these results showed that all three polysaccharides induced macrophage activation, which in turn promoted NO secretion. It was similar to previous studies of Lan et al (2021), where they extracted a polysaccharide named LPsx from dried "Shixia" longan and found that NO production was positively correlated with the concentration of LPsx, suggesting that LPsx promoted NO secretion by macrophages. Similarly, Li et al (2020) found that MOP-3, a polysaccharide extracted from Moringa oleifera leaves, significantly promoted NO secretion from RAW264.7 cells in a dose-dependent manner within a certain concentration range, and iNOS mRNA expression levels were significantly increased.…”

Section: 83supporting

confidence: 90%

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Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Liu

Mao

Zhang

et al. 2022

Journal of Food Science

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In this study, gradient ethanol precipitation method was applied to obtain the extracellular polysaccharides of Lactobacillus paracasei VL8 (VL8‐EPS). The yields, physicochemical properties, and immunomodulatory effects of VL8‐EPS obtained by precipitation at different ethanol concentrations (30%, 50%, and 70%, v/v) were compared. The results showed that VL8‐EPSs were high molecular weight sulfated heteropolysaccharides, composed mainly of glucose and galactose, and the alteration of ethanol concentration had an effect on their chemical compositions, molecular weight distributions, monosaccharide composition, and surface structure, while the primary structure remained the same. Among the three polysaccharide fractions, VL8‐EPS50 displayed better immunomodulatory activities compared with VL8‐EPS30 and VL8‐EPS70. VL8‐EPS50 was found to exert immunomodulatory effects by enhancing the phagocytic activity of RAW264.7 cells and to promote their secretion of more nitric oxide; it also showed stronger thermal and solution stability. In summary, there was a correlation between the structural characteristics of polysaccharides and their immunomodulatory activity, and VL8‐EPS50 was preferentially used for in vivo immunomodulatory activity. Practical ApplicationThis study opens up the source of raw materials for functional foods, which can provide some theoretical basis for the research and development of extracellular polysaccharides of lactic acid bacteria and promote their application in the future development of food industry.

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Section: Resultssupporting

confidence: 91%

Section: 83supporting

confidence: 90%

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Liu

Mao

Zhang

et al. 2022

Journal of Food Science

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“…81 CR3 is one of the major macrophage receptors which mediate phagocytosis and cytotoxic chemokine release. 82 Previous studies indicated that polysaccharides containing glucose, N-acetyl-D-glucosamine, or mannose are most likely to be recognized by CR3. [82][83][84] Therefore, the mannose units of SMSP2 may contribute to its recognition by macrophages CR3.…”

Section: Papermentioning

confidence: 99%

Immunomodulatory activity ofSenegalia macrostachya(Reichenb. ex DC.) Kyal. & Boatwr seed polysaccharide fraction through the activation of the MAPK signaling pathway in RAW264.7 macrophages

et al. 2022

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“…These twelve correlation peaks in the anomeric region of AVPG-2, including δ 4.62/95.84 ppm, δ 5.40/99.70 ppm, δ 5.39/101.63 ppm, δ 5.22/108.27 ppm, δ 4.41/103.09 ppm, δ 5.23/109.18 ppm, δ 5.20/92.01 ppm, δ 5.07/98.92 ppm, δ 4.49/102.72 ppm, δ 4.71/100.70 ppm, δ 5.26/97.61 ppm and δ 5.06/107.43 ppm (denoted as A , B , C , D , E , F , G , H , I , J , K and L , respectively), revealed that there were twelve sugar residues in AVPG-2. The assignment of chemical shifts of these residues were determined according to 1 H, 13 C, HSQC, 1 H- 1 H COSY and HMBC spectra, as well as data reported in the literature [ 40 , 43 , 44 , 46 , 47 , 48 , 49 ] and the results of methylation analysis, as shown in Table 2 . The linkage sites and sequence among the sugar residues of AVPG-2 were also analyzed according to the HMBC spectra ( Figure 6 E).…”

Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

et al. 2021

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Amomum Villosum Lour. (A. villosum) is a folk medicine that has been used for more than 1300 years. However, study of the polysaccharides of A. villosum is seriously neglected. The objectives of this study are to explore the structural characteristics of polysaccharides from A. villosum (AVPs) and their effects on immune cells. In this study, the acidic polysaccharides (AVPG-1 and AVPG-2) were isolated from AVPs and purified via anion exchange and gel filtration chromatography. The structural characteristics of the polysaccharides were characterized by methylation, HPSEC-MALLS-RID, HPLC, FT-IR, SEM, GC-MS and NMR techniques. AVPG-1 with a molecular weight of 514 kDa had the backbone of → 4)-α-d-Glcp-(1 → 3,4)-β-d-Glcp-(1 → 4)-α-d-Glcp-(1 →. AVPG-2 with a higher molecular weight (14800 kDa) comprised a backbone of → 4)-α-d-Glcp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-d-Glcp-(1 →. RAW 264.7 cells were used to investigate the potential effect of AVPG-1 and AVPG-2 on macrophages, and lipopolysaccharide (LPS) was used as a positive control. The results from bioassays showed that AVPG-2 exhibited stronger immunomodulatory activity than AVPG-1. AVPG-2 significantly induced nitric oxide (NO) production as well as the release of interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α), and upregulated phagocytic capacities of RAW 264.7 cells. Real-time PCR analysis revealed that AVPG-2 was able to turn the polarization of macrophages to the M1 direction. These results suggested that AVPs could be explored as potential immunomodulatory agents of the functional foods or complementary medicine.

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Glucose-rich polysaccharide from dried ‘Shixia’ longan activates macrophages through Ca2+ and CR3- mediated MAPKs and PI3K-AKT pathways

Cited by 27 publications

References 47 publications

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Immunomodulatory activity ofSenegalia macrostachya(Reichenb. ex DC.) Kyal. & Boatwr seed polysaccharide fraction through the activation of the MAPK signaling pathway in RAW264.7 macrophages

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

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