Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium

Kim, J.H.; Funahashi, Hiroaki; Niwa, K.; Okuda, Kiyoshi

doi:10.1016/0093-691x(93)90425-5

Cited by 73 publications

(39 citation statements)

References 33 publications

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“…The ciliated cells of the oviduct are lacking in GLUT1, whose immunoreactivity is restricted to the basolateral membrane of grouped non-ciliated cells (82). Zygotes and early embryos utilize lactate and pyruvate before implantation, while glucose is a major energy source from the blastocyst stage onwards (74,138). In the uterus, both epithelial cells facing the lumen of the uterus and uterine gland cells express MCT1 but not MCT4 along their basolateral membrane.…”

Section: The Female Genital Organmentioning

confidence: 99%

Cellular distributions of monocarboxylate transporters: a review

Iwanaga

Kishimoto

2015

Biomed. Res.

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Lactate and ketone bodies play important roles as alternative energy substrates, especially in conditions with a decreased utility of glucose. Short-chain fatty acids (acetate, propionate, and butyrate), produced by bacterial fermentation, supply most of the energy substrates in ruminants such as the cow and sheep. These monocarboxylates are transfered through the plasma membrane by proton-coupled monocarboxylate transporters (MCTs) and sodium-coupled MCTs (SMCTs). To reveal the metabolism and functional significance of monocarboxylates, the cellular localization of MCTs and SMCTs together with the expressed intensities holds great importance. This paper reviews the immunohistochemical localization of SMCTs and major MCT subtypes throughout the mammalian body. MCTs and SMCTs display a selective membrane-bound localization with porality. In contrast to the limited expression of SMCTs in the intestine and kidney, MCTs display a broader distribution pattern than GLUTs. The brain, kidney, placenta, and male genital tract express multiple subtypes of the MCT family. Determination of the cellular localization of MCTs is most controversial in the brain, possibly due to regional differences and the transcriptional modification of MCT proteins. Information on the localization of MCTs and SMCTs aids in understanding the nutrient absorption and metabolism throughout the mammalian body. In some cases, the body may use monocarboxylates as signal molecules, like hormones.

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Section: The Female Genital Organmentioning

confidence: 99%

Cellular distributions of monocarboxylate transporters: a review

Iwanaga

Kishimoto

2015

Biomed. Res.

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“…mintr and Bavister, 1996 . Thus, the stimulatory effect of glucose is generally described Ž in association with its delayed incorporation in culture Kim et al, 1993a;Lim et al, . 1994a;Ohboshi et al, 1996;Furnus et al, 1997;Donnay et al, 1999 . Factors required to reach the morula and blastocyst stage may be lacking in oviductal factors from some batches of conditioned medium, and limited development to the Ž blastocyst stage of such cultured embryos was observed in preliminary studies .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, glucose at concentrations ranging from 1.5t o 5.56 mM, has been reported to improve blastocyst production both in presence of Ž serum Rorie et al, 1994;Carolan et al, 1995;Koji et al, 1997;Wang et al, 1997; . Ž Donnay et al, 1999 and in the presence of BSA Kim et al, 1993a;Lim et al, 1993Lim et al, , . 1994aOhboshi et al, 1996;Furnus et al, 1997 .…”

Section: Introductionmentioning

confidence: 99%

Effects of glucose and protein sources on bovine embryo development in vitro

Gómez

Díez

2000

Animal Reproduction Science

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Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct Ž . Ž . Ž . Ž . fluid mSOF alone or supplemented with 1 oviductal factors, 2 BSA and 3 FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and Ž . FCS. Glucose 1.5 mM added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA. q

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“…Co-culture with somatic cells affects embryo development-not only secretion of growth factors but also glucose reduction caused by their metabolism [7,27]. Kim et al [17] reported that supplementation TCM199 with 5.56 mM glucose suppressed embryo development, and the same effect of glucose was reported in other culture media [18,22,30,34]. Therefore, it is considered that glucose as a component of TCM199 delays and reduces the development of embryos in vitro.…”

Section: Discussionmentioning

confidence: 88%

Different Factors Affect Developmental Competence and Cryotolerance in in vitro Produced Bovine Embryo.

Imai

Matoba

Dochi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. The present study was conducted to examine the effects of culture systems and culture media on developmental competence and freezability of bovine embryos obtained by in vitro culture of in vitro matured and fertilized (IVM-IVF) oocytes. No significant difference was observed in the proportions of oocytes developed to blastocysts, the speed at which the oocytes reached the blastocyst stage and the number of cells, when the IVM-IVF oocytes were cultured in CR1aa with or without cumulus cells. Nevertheless, more of the IVM-IVF oocytes cultured either with or without cumulus cells in CR1aa were seen to reach the blastocyst stage much sooner than those cultured with cumulus cells in TCM199 (P<0.05). The proportion of embryos developed to the blastocyst stage by day 7 in CR1aa culture was significantly higher than embryos cultured in TCM199. Viability after frozen-thawed blastocysts were obtained in vitro, was seen in a significantly higher percentage of embryos cultured in TCM199 and developed to the hatched blastocysts than in those cultured in CR1aa (P<0.05). These results indicate that CR1aa was superior to TCM199 for the potential developmental of IVM-IVF oocytes to blastocysts during in vitro culture regardless of co-culture with or without cumulus cells. But the freezability of blastocysts developed in CR1aa was inferior to those developed in TCM199. KEY WORDS: culture medium, developmental competence, freezability, in vitro culture, in vitro produced bovine embryo.J. Vet. Med. Sci. 64(10): 887-891, 2002 In vitro culture of bovine embryos derived from IVM-IVF have succeeded in producing calves [10,21]. An adequate in vitro culture system for bovine zygotes is required for large scale embryo production by IVM-IVF and genetic improvement by means of ovum pick-up and IVM-IVF. This culture system is used for the development and efficient utilization of embryonic techniques in cattle, for example for embryonic or somatic cell nuclear transfer and in transgenic animals. Bovine IVM-IVF zygotes were first cultured with cumulus cells [8,10], oviduct epithelial cells [9,21], granulosa cells [13], amnion cells [2] and buffalo rat liver cells [36] in TCM199 supplemented with bovine serum, but there are many differences between in vitro produced bovine embryos and those produced in vivo. It was reported that in vitro produced embryos have darker cytoplasm and lower density [24,25]. CR1aa medium was developed for in vitro culture of bovine IVM-IVF zygotes [15,29,30], but the optimum culture system for IVM-IVF oocytes and freezability of blastocysts cultured in CR1aa still remain to be clarified. The chilling sensitivity should be considered as a factor affecting the quality of in vitro produced bovine blastocysts.The aim of the present study was to examine the effects of culture systems and culture media on the development competence and the quality of bovine embryos obtained by in vitro culture of IVM-IVF oocytes. MATERIALS AND METHODS Collection of oocytes and in vitro maturation:Bovine ovaries were obtained ...

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Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium

Cited by 73 publications

References 33 publications

<b>Cellular distributions of monocarboxylate transporters: a r</b><b>eview </b>

<b>Cellular distributions of monocarboxylate transporters: a r</b><b>eview </b>

Effects of glucose and protein sources on bovine embryo development in vitro

Different Factors Affect Developmental Competence and Cryotolerance in in vitro Produced Bovine Embryo.

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