Glucose released by hydrolytic activity of amylase influences the pigment synthesis in <i>Penicillium</i> sp NIOM‐02

Puttananjaiah, Mohankumari Honganoor; Dhale, Mohan Appasaheb

doi:10.1002/jobm.201100461

Cited by 6 publications

(6 citation statements)

References 28 publications

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“…In Bacillus subtilis KCC103, the α‐amylase synthesis is not subject to catabolite repression, it is observed that the supplementation of biomass sugars, glucose or xylose at 20% (w/w), did not repress the synthesis of α‐amylase , these results are similar to those observed for the production of glucoamylase by A. phoenicis . According to Puttananjaiah and Dhale the carbon catabolite repression is generally considered as a regulatory mechanism to ensure sequential synthesis of secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis

Benassi

Pasin

Facchini

et al. 2013

J. Basic Microbiol.

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This study investigates the production of glucoamylase from Aspergillus phoenicis in Machado Benassi (MB) medium using 1% maltose as carbon source. The maximum amylase activity was observed after four days of cultivation, on static conditions at 30 °C. Glucoamylase production was induced by maltose and inhibited by different glucose concentrations. The optimum of temperature and pH were 60-65 °C, and 4.5 or 5.0 to sodium acetate and Mcllvaine buffers, respectively. It was observed that the enzyme was totally stable at 30-65 °C for 1 h, and the pH range was 3.0-6.0. The enzyme was mainly activated by manganese (176%), and calcium (130%) ions. The products of starch hydrolysis were analyzed by thin layer chromatography and after 3 h, only glucose was detected, characterizing the amylolytic activity as a glucoamylase.

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Section: Discussionmentioning

confidence: 99%

A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis

Benassi

Pasin

Facchini

et al. 2013

J. Basic Microbiol.

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“…1a, 2a). However, Puttananjaiah and Dhale (2012) reported that increased amylase activity promoted glucose release from starch at pH 3.0, which led to reduced pigment synthesis in Penicillium sp. NIOM-02 because of carbon catabolite repression.…”

Section: Discussionmentioning

confidence: 99%

Highly efficient improvement of Monascus pigment production by accelerating starch hydrolysis in Monascus ruber CICC41233

et al. 2018

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To investigate the relationship between starch hydrolysis and pigments (MPs) production, the α-amylase gene () from was heterologously expressed in CICC41233, and we obtained a positive transformant named Amy9. In Amy9, the α-amylase activities were 6.65- and 4.26-fold higher at 72 h and 144 h, respectively, than those in the parent strain with the glucose as solo carbon medium. Surprisingly, in the MPs fermentation medium with rice powder as solo material, Amy9 completely degraded starch at 48 h, while 43.93 and 7.29 mg/mL starch remained at 48 and 144 h, respectively, in the parent strain. Amy9 accelerated starch hydrolysis, which enhanced biomass and also increased total MPs by 132% after 144 h. Compared with CICC41233, the relative gene expression levels, as determined by a quantitative real-time polymerase chain reaction analysis, of encoding ATP-citrate lyase subunit 2, encoding polyketide synthase, and encoding the fatty acid synthase beta subunit increased by 33.14, 145.18, and 32.15%, respectively, after 144 h in Amy9. The up-regulated expression of these key genes in MPs synthesis contributed to the large increase in MPs production. This interesting work provided us with a new idea and a new target for the study of the MPs production.

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“…The fungus Penicillium sp. NIOM-02 showed an increase of amylase activity at cultivation on wheat and corn flour by submerged fermentation (SmF) (246 U mg -1 ) and by SSF (18 U mg -1 ) in comparison with the activity on sugar cane bagasse ( Dhale and Vijay-Raj, 2009 ). Remarkably, the addition of higher quantities of corn flour or corn steep liquor (≥30 g L -1 ) as the substrate repressed the amylase production during SmF.…”

Section: Amylolytic Enzymesmentioning

confidence: 99%

“…Remarkably, the addition of higher quantities of corn flour or corn steep liquor (≥30 g L -1 ) as the substrate repressed the amylase production during SmF. Moreover, an acidic condition did not stimulate the fungus metabolite production, inducing only stress-dependent sporulation, probably, due to the marine origin of the strain, where pH is slightly alkaline ( Dhale and Vijay-Raj, 2009 ).…”

Section: Amylolytic Enzymesmentioning

confidence: 99%

Biotechnology Potential of Marine Fungi Degrading Plant and Algae Polymeric Substrates

et al. 2018

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Filamentous fungi possess the metabolic capacity to degrade environment organic matter, much of which is the plant and algae material enriched with the cell wall carbohydrates and polyphenol complexes that frequently can be assimilated by only marine fungi. As the most renewable energy feedstock on the Earth, the plant or algae polymeric substrates induce an expression of microbial extracellular enzymes that catalyze their cleaving up to the component sugars. However, the question of what the marine fungi contributes to the plant and algae material biotransformation processes has yet to be highlighted sufficiently. In this review, we summarized the potential of marine fungi alternatively to terrestrial fungi to produce the biotechnologically valuable extracellular enzymes in response to the plant and macroalgae polymeric substrates as sources of carbon for their bioconversion used for industries and bioremediation.

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Glucose released by hydrolytic activity of amylase influences the pigment synthesis in Penicillium sp NIOM‐02

Cited by 6 publications

References 28 publications

A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis

A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis

Highly efficient improvement of Monascus pigment production by accelerating starch hydrolysis in Monascus ruber CICC41233

Biotechnology Potential of Marine Fungi Degrading Plant and Algae Polymeric Substrates

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