Glucose protection against [14C] N‐ethylmaleimide labelling of a protein in galactose‐transporting membrane vesicles of Escherichia coli

Kaethner, T. M.; Horne, P

doi:10.1016/0014-5793(80)80605-3

Cited by 3 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Membrane vesicles were then prepared as described by Kaback (1971) from spheroplasts made by the method of Witholt et al (1976). Vesicle preparations were essentially free of intact cells or spheroplasts as determined by phase-contrast light or electron microscopy and contained less than 2% of contaminating cytoplasmic enzymes (Home, 1980). Vesicles were either used fresh or frozen in liquid N2 immediately after preparation and stored at -200C before use.…”

Section: Experimental Procedures Growth Ofcells Andpreparation Ofvesimentioning

confidence: 99%

The association of proton movement with galactose transport into subcellular membrane vesicles of Escherichia coli

Horne¹,

Henderson²

1983

Biochemical Journal

View full text Add to dashboard Cite

1. Subcellular membrane vesicles were prepared from a strain of Escherichia coli constitutive for the GalP galactose-transport system. 2. The addition of substrates of the GalP transport system to vesicle suspensions promoted alkaline pH changes, which provided direct evidence for the coupling of sugar and proton transport. 3. Respiration-energized galactose transport was progressively inhibited at pH values above 6.0, and was abolished by agents that render the membrane permeable to protons. 4. The combined effects of valinomycin, the nigericin-like compound A217 and pH on galactose transport suggested that both delta pH and delta psi components of the protonmotive force contributed to energization of galactose transport. 5. These results substantiate the conclusion that the GalP transport system operates by a chemiosmotic mechanism.

show abstract

Section: Experimental Procedures Growth Ofcells Andpreparation Ofvesimentioning

confidence: 99%