Glucose oxidase-polypyrrole electrodes synthesized in p-toluenesulfonic acid and sodium p-toluenesulfonate

Özyılmaz, Gül; Özyılmaz, A.T.; Can, Faruk

doi:10.1134/s0003683811020153

Cited by 8 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[4][5][6][7][8][9] Fabrication of the enzyme containing biosensor requires immobilization of the enzyme molecule onto/into the electrode surface. There are several strategies for immobilization techniques to obtain GOD biosensor such as crosslinking with glutaraldehyde, [10][11] applying enzyme on an electrode in a gel film, [12][13][14][15][16][17] entrapment or incorporation in a polymer matrix during electropolymerization, 6,[18][19][20] by covalent attachment [21][22] or adsorbing onto electrode surface. [24][25][26] Chitosan (Chi) is a type of natural cationic polymer, which has shown attractive characteristics such as film-forming ability, permeability, and good adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…31 Electropolymerization is one of the cheap but powerful methods focusing on selective modification of various types of electrodes with desired matrices. 3 Several electropolymers were used to fabricate the glucose biosensor such as PPy 18,28,[32][33] polyaniline (PANI), [34][35] poly(o-anisidine) (POA), [36][37] poly(N-methyl pyrrole) (PNMP), 27 poly(o-phenylenediamine) 38 and polythophen 39 derivative. Recent studies reveal that the nanostructured metal oxides with reduced size have unique advantages in immobilizing enzymes and have high sensitivity due to high surface area, desirable microenvironment, and direct electron transfer between the enzyme active sites and electrode.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Özyılmaz

Bayram

et al. 2019

ACSi

View full text Add to dashboard Cite

GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Özyılmaz

Bayram

et al. 2019

ACSi

View full text Add to dashboard Cite

show abstract

“…To this, conductive polymers which have redox center are widely used (Bal 2012). There are several studies about biosensors which are prepared using conductive polymers such as polypyrrole (Sung et al 2003, Ozyilmaz et al 2011, Chen et al 2005, Ma et al 2003, polyaniline (Borole et al 2004, Zejun et al 2014, Jie et al 2015, polythiophen (Kawai et al 1990), polyparaphenylene (Ivory et al 1979), poly(N-methylpyrrole) (Miyamoto et al 1990, Ozyilmaz et al 2017. Among them, polypyrrole is mostly used conductive polymer due to easy preparation, stability and high conductivity (Ahuja et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Özyılmaz¹,

Özyılmaz²,

Ağçam³

2018

Natural and Engineering Sciences

View full text Add to dashboard Cite

In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on platinum electrode with 0.09 cm 2 surface area which coated with polypyrrole (PPy) by cyclic voltammetry technique. Because measured current values in the presence of glucose would be affected from the electrode preparing and working conditions, experimental parameters should be optimized by response surface methodology (RSM). To this, State Ease Design Expert 8.0.7.1. (Serial Number:0021-6578) programe was used. PPy synthesis conditions of pyrrole (Py) monomer concentration and scan rate were optimized according to current response in presence of glucose. Optimal Py monomer concentration and scan rate for PPy synthesis were determined as 10 mM and 50 mV/s, respectively. Immobilization parameters such as concentrations of chitosan, GOD and glutaraldehyde (GAL) also were optimized by RSM as 1.0 %, 4 mg/ml and 0.0625 %, respectively. The digital photos of electrodes at each stage were obtained. All electrodes well characterized in absence and in the presence of glucose by cyclic voltammetry and impedance techniques and it was observed that electrodes were sensitive to glucose molecule. Finally the effect of working pH and applied potential on the current response was investigated by RSM. The highest current response was obsreved when pH of glucose solution and applied potential were 6.0 and 0.8, respectively.

show abstract

“…Construction of the enzyme containing biosensor is required immobilization of protein molecule onto/into the electrode which plays an important role in the research of glucose biosensor. There are several strategies on immobilization techniques to get GOD biosensor such as crosslinking with glutaraldehyde (Kadam et al(all the et al should be in italic form) 2011, Shkotova et al 2016), applying on an electrode with a gel matrix (Huang et al 2012, Pauliukaite et al 2010, Haighi et al 2012, entrapment or incorporation in polymer matrix during electropolymerization (Ozyilmaz et al 2011, Uang et al2003, Eftekhari 2004, by covalent attachment (Abu-Rabeah et al 2009, Ekiz et al 2011, Shervedani et al 2007 or adsorbed on electrode surface (Guadalupe de Jesus et al 2013, Salimi et al 2011, Jiang et al 2012. Chitosan is a type of natural cationic polymer, which has shown attractive characteristics such as film-forming ability, permeability, and good adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, chitosan can be used as a gel matrix for enzyme immobilization through GAL or another reagent . Several electropolymers were used to fabricate the glucose biosensor such as polypyrrole (PPy) (Ozyilmaz et al 2011, Raicopol et al 2013, Olea et al 2008, polyaniline (PANI) (Ozdemir et al 2010, Yao et al 2015, Tang et al 2015, poly(o-anisidine) (POA) (Savale & Shirsat 2009, Borole et al 2007), poly(o-phenylenediamine) (Rothwell et al 2010), polythophen derivative (Abasiyanik et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

Özyılmaz¹,

Özyılmaz²,

Akyürekoğlu³

2017

Natural and Engineering Sciences

View full text Add to dashboard Cite

In this study, Pt electrode was coated by poly(N-methypyrrol) (PNMP) film, then Glucose Oxidase (GOD) was immobilized onto PNMP layer with thin chitosan (Chi) gel and finally, electrode was reacted with glutaraldehyde (GAL) to form crosslinking between -NH2 groups of Chi and GOD to prevent enzyme leakage from Chi. GOD-based electrode was used to measure current response depending on glucose concentration by chronoamperometric method. Due to preparation of electrode conditions have significant effect on current values which were measured and optimized in presence of glucose, polymer synthesis and GOD immobilization conditions detailed. Therefore, the effect of N-methylpyrrole monomer concentration, scan rate, Chi concentration, GOD concentration and GAL concentration on biosensor response was investigated by classical method. In sight of obtained data, optimal monomer concentration and scan rates for PNMP synthesis were determined as 50 mM and 20 mV/s, respectively. Optimal Chi, GOD and GAL concentrations were found as 1,00%, 4 mg/mL and 0.025 %, respectively. SEM images of Pt, PNMP coated Pt and GOD immobilized Pt electrodes were obtained; Imax and KM values were calculated using Lineweaver-Burk plot. After 20 successive uses of same enzyme electrode in 5 mM glucose solution, it kept still its 91.3 % of initial activity.

show abstract

Glucose oxidase-polypyrrole electrodes synthesized in p-toluenesulfonic acid and sodium p-toluenesulfonate

Cited by 8 publications

References 33 publications

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

Contact Info

Product

Resources

About